Methodology to streamline flow cytometric-based detection of early stage Plasmodium parasitemia in mice

Liu, Mohan; Liao, Matthew J.; Fisher, Christopher J.; Miguel, Rodolfo D. Vicetti; Cherpes, Thomas L.

doi:10.1016/j.mimet.2022.106439

Journal of Microbiological Methods

2022

DOI: 10.1016/j.mimet.2022.106439

|View full text |Cite

Methodology to streamline flow cytometric-based detection of early stage Plasmodium parasitemia in mice

Mohan Liu

Matthew J. Liao

Christopher J. Fisher

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Probing novel epitopes on the Plasmodium falciparum circumsporozoite protein for vaccine development

Krenger,

Roques,

Vogt

et al. 2024

npj Vaccines

View full text Add to dashboard Cite

RTS,S and R21 are the only vaccines recommended by the WHO to protect children from Plasmodium falciparum (Pf) clinical malaria. Both vaccines target the Pf sporozoite surface protein circumsporozoite protein (CSP). Recent studies showed that human antibodies neutralize Pf sporozoites most efficiently when simultaneously binding to the PfCSP NANP repeat and the NPDP junction domain. However, neither RTS,S nor R21 targets this junction domain. To test the potential of the NPDP junction domain and other sites of PfCSP as innovative vaccine targets, we developed multiple vaccine candidates based on cucumber mosaic virus-like particles (CuMVTT-VLPs). These candidates vary in several aspects: the number of targeted NANP repeats, the presence or absence of the junction domain, the cleavage site, and up to three NVDP repeats within the target sequence. Immunogenicity and efficacy studies were conducted in BALB/c mice, utilizing chimeric Plasmodium berghei (Pb) sporozoites, in which the endogenous CSP has been replaced by PfCSP (Pb/PfCSP). We observed a positive association between the number of targeted NANP repeats and the induction of specific IgM/IgG antibodies. Elevated humoral responses led to enhanced protection against parasitemia after Pb/PfCSP sporozoite challenge. Especially high-avidity/affinity antibody formation and vaccine protection were NANP repeat-dependent. Intriguingly, vaccine efficacy was not enhanced by targeting sites on PfCSP other than the NANP repeats. Our data emphasize the dominant role of the NANP repeat region for induction of protective antibodies. Furthermore, we present here novel malaria vaccine candidates with an excellent immunogenic profile that confer sterile protection in mice, even in absence of adjuvants.

show abstract

Probing novel epitopes on the Plasmodium falciparum circumsporozoite protein for vaccine development

Krenger,

Roques,

Vogt

et al. 2024

npj Vaccines

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Methodology to streamline flow cytometric-based detection of early stage Plasmodium parasitemia in mice

Cited by 1 publication

References 26 publications

Probing novel epitopes on the Plasmodium falciparum circumsporozoite protein for vaccine development

Probing novel epitopes on the Plasmodium falciparum circumsporozoite protein for vaccine development

Contact Info

Product

Resources

About