Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
The process of obtaining initial pear plants is considered. The technological process includes the selection of plants of a certain variety according to pomological, physiological qualities and productivity; diagnostics for the presence of viruses, phytoplasmas and other harmful organisms by ELISA, PCR and indicators testing; in the absence of healthy plants – release from pathogens by methods of thermotherapy, chemotherapy, in vitro culture, magnetotherapy with re-testing. After preliminary testing in greenhouse conditions, candidate plants for initial plants are obtained, which are then subjected to testing using a set of diagnostic methods. Plants free from the main harmful viruses and phytoplasma are transferred to the category of “initial plants”, and if all the tested plants are infected, they are subjected to recovery. Dry air thermotherapy in combination with grafting of apexes on virus-free rootstocks provides the possibility of obtaining healthy plants during one growing season. For chemotherapy, along with reference drugs (ribavirin), the use of phenolic compounds (salicylic, gallic acids) is promising, which can increase the efficiency of plant recovery from viruses by an average of 28–30%, reduce the cost of the recovery process and improve workplace safety. The use of magnetic pulse processing increases the environmental safety of the technology in the absence of the phytotoxic effect. After diagnosis by a complex of methods in the absence of viruses, pear plants receive the category “initial plant” and are further propagated by budding or grafting.
The process of obtaining initial pear plants is considered. The technological process includes the selection of plants of a certain variety according to pomological, physiological qualities and productivity; diagnostics for the presence of viruses, phytoplasmas and other harmful organisms by ELISA, PCR and indicators testing; in the absence of healthy plants – release from pathogens by methods of thermotherapy, chemotherapy, in vitro culture, magnetotherapy with re-testing. After preliminary testing in greenhouse conditions, candidate plants for initial plants are obtained, which are then subjected to testing using a set of diagnostic methods. Plants free from the main harmful viruses and phytoplasma are transferred to the category of “initial plants”, and if all the tested plants are infected, they are subjected to recovery. Dry air thermotherapy in combination with grafting of apexes on virus-free rootstocks provides the possibility of obtaining healthy plants during one growing season. For chemotherapy, along with reference drugs (ribavirin), the use of phenolic compounds (salicylic, gallic acids) is promising, which can increase the efficiency of plant recovery from viruses by an average of 28–30%, reduce the cost of the recovery process and improve workplace safety. The use of magnetic pulse processing increases the environmental safety of the technology in the absence of the phytotoxic effect. After diagnosis by a complex of methods in the absence of viruses, pear plants receive the category “initial plant” and are further propagated by budding or grafting.
The dry-air thermotherapy for fruit and berry crop recovery from harmful viruses has become a widely used technology. The method allows virus-free plants to be obtained within one year. The present work examines the heat resistance of pear plants during the recovery from major harmful viruses using dry-air thermotherapy. The method was employed on the pear plants of Belorusskaya Pozdnyaya, Bryanskaya Belitsa, Velesa, Letnyaya Zabava, Zolotoy Vityaz, Aurora, Gera, Chizhovskaya varieties, R-11-9, R-10-3, R-2-4 hybrids and Zagoryevskii rootstock in 2020-2021 at a temperature of 38 °C for 90 days. The recovery was carried out against harmful latent viruses of apple-tree stem pitting (ASGV), apple brown spot (ASPV), chlorosis (ACLSV), apple mosaic virus (ApMV). For thermotherapy, a temperature chamber designed at the FSBO ARHCBAN, having a temperature gradient for root and elevated systems, drip watering, illumination by LED fixtures, was used. The transpiration rate, leaf water content, water loss, water deficiency and recovery of water content were determined. The plant growth parameters of different pear varieties depended on the duration of thermotherapy and varietal features. The Velesa, Zolotoy Vityaz varieties and Zagoryevskii rootstock generated the longest shoots. As compared with winter greenhouse conditions, the transpiration rate decreased 2.1 times, the total water content in leaves increased 1.5 times, the water loss upon heat shock decreased 1.6 times, and the recovery of water content increased 1.8 times. The Bryanskaya Krasavitsa varieties and R-11-9 and R-10-3 hybrids exhibited the highest heat resistance during cultivation in a heat chamber; the Letnyaya Zabava and Zolotoy Vityaz varieties exhibited medium heat resistance; the Belorusskaya Pozdnyaya variety, R-2-4 hybrid and Zagoryevskii rootstock were characterised by low heat resistance. A high positive correlation (r = 0,92) between total water content in leaves and the recovery of water content following heat shock was established. The yield of ASGV, ASPV, ACLSV, ApMV virus-free pear plants in the year following dry-air heat treatment amounted to 83 %.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.