Methods for assessing biochemical oxygen demand (BOD): A review

Jouanneau, Sulivan; Recoules, Loic; Durand, M.; Boukabache, Ali; Picot, Vincent; Primault, Yves; Lakel, Amar; Sengelin, M.; Barillon, B.; Thouand, Gérald

doi:10.1016/j.watres.2013.10.066

Cited by 346 publications

(168 citation statements)

References 109 publications

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“…Dose response measurements revealed two compounds with sub-micromolar potencies: OUL47 (resorufin, 7-hydroxy-3H-phenoxazin-3-one, NSC12097) (IC 50 = 482 nM) and OUL35 (4-[(4-carbamoylcyclohexyl)oxy]cyclohexane-1-carboxamide, NSC39047) (IC 50 = 329 nM) ( Figures 1A and 1B). OUL47 is widely used as an indicator of cell viability in assays where resazurin is reduced to the highly fluorescent OUL47 by living cells (Jouanneau et al, 2014).…”

Section: Discovery Of a Potent And Selective Artd10 Inhibitormentioning

confidence: 99%

Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage

Venkannagari

Verheugd

Koivunen

et al. 2016

Cell Chemical Biology

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SUMMARYMembers of the human diphtheria toxin-likeADP-ribosyltransferase (ARTD or PARP) family play important roles in regulating biological activities by mediating either a mono-ADP-ribosylation MARylation) of a substrate or a poly-ADP-ribosylation (PARylation). ARTD10/PARP10 belongs to the MARylating ARTDs (mARTDs) subfamily, and plays important roles in biological processes that range from cellular signaling, DNA repair, and cell proliferation to immune response. Despite their biological and disease relevance, no selective inhibitors for mARTDs are available. Here we describe a small-molecule ARTD10 inhibitor, OUL35, a selective and potent inhibitor for this enzyme. We characterize its selectivity profile, model its binding, and demonstrate activity in HeLa cells where OUL35 rescued cells from ARTD10 induced cell death. Using OUL35 as a cell biology tool we show that ARTD10 inhibition sensitizes the cells to the hydroxyurea-induced genotoxic stress. Our study supports the proposed role of ARTD10 in DNA-damage repair and provides a tool compound for selective inhibition of ARTD10-mediated MARylation.3

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Section: Discovery Of a Potent And Selective Artd10 Inhibitormentioning

confidence: 99%

Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage

Venkannagari

Verheugd

Koivunen

et al. 2016

Cell Chemical Biology

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“…The DO is measured at the initial time, before to hermetically close the flask, and after the incubation period in darkness. Initially the DO was measured by titrimetric analysis which was replaced later with electrochemical probes [3]. The EN and ISO standards are now considering the DO measurement using optical probes.…”

Section: Standard Analytical Methodsmentioning

confidence: 99%

“…Some national standards also described a manometric method for BOD measurement as the Norwegian NS 4758 and the American 5210-D standards. In this case, the hermetically closed flask shows a gaseous headspace and oxygen uptake is appraised by the air pressure decrease in presence of sodium hydroxide pellets [3]. These pellets insure the removal of gaseous carbon dioxide resulting from bacterial respiration.…”

Section: Bod(tt) = Ubod [1-e -K(t) T ]mentioning

confidence: 99%

“…However, for an efficient management of biological treatment processes which requires highly responsive analytical tools, a 5 days incubation period is too long. Moreover, the current BOD measurement methods are really labour-intensive as well as time-and space-consuming [3][4].…”

Section: Regulatory Framework and Shortcomingsmentioning

confidence: 99%

“…However, the analytical methods currently available for the BOD measurement drastically suffer from a lack of operational suitability. These methods are lengthy and tedious while the operation of modern STP requires a high responsiveness through fast and easy-to-use analytical tools [3][4]. In the last decades, the biological processes for wastewater treatment have substantially changed to match the stricter and stricter effluent quality standards decreed by the European authorities.…”

Section: Introductionmentioning

confidence: 99%

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New outlook in BOD measurement and bioprocess management: the Enverdi® kit

Müller¹,

Bellaton²,

Yoris³

et al. 2017

18th International Congress of Metrology

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Abstract. Knowing the quantity and biodegradability of wastewater organic matter (OM) is really essential for the optimization of modern biological treatment processes, particularly when considering the highly challenging context for sewage treatment plant operators. However, the current standard methods for wastewater characterisation, as for 5-days biochemical oxygen demand (BOD5) measurement, OM fractionation analysis and bacterial inhibitor detection, show very poor suitability for routine operational use. In this paper, the efficiency of the commercial Enverdi® method (fluorimetric respirometry) for such analytical applications is investigated, especially for the BOD5-equivalent measurement in municipal wastewater samples. The result of numerous experiments showed that the BOD5 values obtained from the alternative Enverdi® method and the main standard methods are statistically equivalent. The analytical performances of the Enverdi® method was found similar to the standard BOD5 method specifications and perfectly matched the French regulatory requirements in force. The regulatory acceptance of this method is presently under consideration. Such an outlook would be really valuable for end-users given the main advantages of this analytical solution which is time-and space-saving, user-friendly and financially competitive. In addition to BOD5 measurement, the fluorimetric respirometry displayed very promising abilities for the OM fractionation and the detection of bacterial inhibitors in wastewater.

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