Methods for cell proliferation analysis by fluorescent image cytometry

Abstract

Methods were developed for multimodal microscopic image analysis in order to identify and analyze one cell type under various microscopic conditions. Our purpose was to quantify both total DNA content using propidium iodide (PI) stain and S-phase fraction using the bromodeoxyuridine (BrdUrd) incorporation technique in cell population subsets. The model chosen was plasma cells in bone marrow triply labelled with fluorescein isothiocyanate (FITC) for intracytoplasmic immunoglobulins, with amino-methylcoumarin-acetate

Development of a Novel PPARγ Ligand Screening System Using Pinpoint Fluorescence-Probed Protein

“…Moreover, even in the case of small fluorescent molecules, it is difficult to introduce them into the desired positions. 15,16) In this context, the four-base codon fluorescence-probing method meets both requirements: low molecular weight (700 Da) and ease of pinpoint probing. Thus, the method is valuable for detecting molecular interactions.…”

Numerical Acquisition of Multiple Immunofluorescence Labelling

“…This is important for 3D spatial distribution and precise colocalization studies, but not in other cases such as total fluorescence quantification, and routine FISH cytogenetics. For instance, integrated fluorescence intensities of cytocentrifuged cells were measured using an intensified CCD camera [7]. Efficient FISH cytogenetic acquisitions were performed using an integrated colour CCD camera, a triple-band filter set and a software that makes it possible to equilibrate the three CCD channels with respect to each other on line (Fig.…”

Microplates with Integrated Oxygen Sensors for Kinetic Cell Respiration Measurement and Cytotoxicity Testing in Primary and Secondary Cell Lines

“…Numerous assays are available for this purposes. [2][3][4] These assays are important tools for in vitro testing in pharmaceutical research, and their scientific as well as their commercial potential cannot be overemphasized. Cell viability is usually assessed by membrane integrity (e.g., trypan blue exclusion) and cell proliferation by labeled nucleotide incorporation (e.g., [ 3 H]thymidine), ATP, or metabolic markers such as MTT, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), etc.…”