Methods for Characterization/Manipulation of Human Corneal Stem Cells and their Applications in Regenerative Medicine

Corradini, Francesca; Venturi, Beatrice; Pellegrini, Graziella; Luca, Michele De

doi:10.1007/978-1-61779-980-8_26

Cited by 15 publications

(11 citation statements)

References 13 publications

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“…Cells were isolated from the fresh specimens as previously described (Corradini et al. ). Briefly, 55 specimens were treated with 0.25% trypsin (GibcoTM/Thermo Fisher Scientific) in 0.03% EDTA solution (GibcoTM/Thermo Fisher Scientific, Paisley, Scotland, UK) at 37°C for 3–4 cycles of 30 min each.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of dendritic cell subtypes in preserved and cultured cadaveric human corneolimbal tissue on amniotic membrane

Lužnik

Kopitar

Lapajne

et al. 2018

Acta Ophthalmologica

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Section: Methodsmentioning

confidence: 99%

Identification and characterization of dendritic cell subtypes in preserved and cultured cadaveric human corneolimbal tissue on amniotic membrane

Lužnik

Kopitar

Lapajne

et al. 2018

Acta Ophthalmologica

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“…Stem cells have also shown potentials in reversing the effects of age, offering a possible ‘cure’ for aging altogether [21]. Stem cells have also been reportedly used to reverse blindness [22] and are currently being investigated for use in the treatment of graft-versus-host disease, Crohn’s and lupus, based on their ability to modulate the immune system [23,24] and in the screening of potential anti-tumor drugs with promising data arising from animal-testing [3]. …”

Section: Introductionmentioning

confidence: 99%

Stem cells in Dentistry: knowledge and attitude of Nigerian Dentists

2013

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BackgroundSeveral controversies exist about the methods of harvesting and eventual utilization of stem cells in Medicine and Dentistry. The objective of the study was to investigate the awareness, attitude and knowledge of the use of stem cells in Dentistry among Nigerian Dentists.MethodsThis descriptive, cross-sectional study was conducted among dentists selected from both private and public health sectors, in some of the major cities in Nigeria.ResultsThe majority of the participants were ≤35 years in age, male, Pentecostal Christians, possessed a postgraduate qualification, had practiced for ≤5 years and were specialists or specializing. In this study, 153(81.0%) of the participants reported awareness about the use of stem cells in dentistry which was significantly associated with qualification and type of practice. Most of the respondents 114 (60.3%) had a poor knowledge of the use of stem cells in Dentistry. This was significantly associated with type of practice and awareness about stem cell use in dentistry but binary logistic regression showed awareness as the only determinant of knowledge. About three-quarters 142 (75.1%) of the participants exhibited positive attitude towards stem cell use. This had a positive non-significant association with knowledge and reported awareness.ConclusionData from this study revealed a high level of awareness, positive attitude to and poor knowledge of the use of stem cells in Dentistry among a cross section of Nigerian Dentists.

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“…Despite dramatic differences in culture techniques, autologous cultured LSC transplantation appears to be associated with similar success rates in various published studies . Of note, with the dissociated LSC culture technique, the graft quality is assessed before transplantation and the success rate is associated with presence of holoclones expressing DeltaNp63alpha in culture . This technology permits further graft to be performed from the original limbal biopsy as LSC are subcultured and stored as frozen dissociated cells.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Results of Cultured Limbal Stem Cell Versus Limbal Tissue Transplantation in Stage III Limbal Deficiency

Borderie

Ghoubay

Georgeon

et al. 2019

Stem Cells Translational Medicine

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We aimed to evaluate efficiency and safety of transplantation of limbal stem cells (LSC) cultured on human amniotic membrane with no feeders and to compare cultured LSC with limbal tissue transplantation. Thirty eyes with stage III LSC deficiency were treated with autologous (autoLSC) or allogeneic (alloLSC) cultured LSC transplantation (prospective phase II clinical trial; average follow-up time, 72 months) or autologous (autoLT) or allogeneic (alloLT) limbal tissue transplantation (retrospective control group; average follow-up time, 132 months) between 1993 and 2014. The 5-year graft survival defined by absence of recurrence of the clinical signs of limbal deficiency was 71% for autoLSC, 0% for alloLSC, 75% for autoLT, and 33% for alloLT. Visual acuity improved by 9.2 lines for autoLSC and 3.3 lines for autoLT. It decreased by 0.7 lines for alloLSC and 1.9 lines for alloLT. Adverse events were recorded in 1/7 autoLSC, 7/7 alloLSC, 6/8 autoLT, and 8/8 alloLT patients. Corneal epithelial defect was the only adverse event recorded after autoLSC, whereas severe sight-threatening adverse events were recorded in the remaining three groups. Compared with failed grafts, successful grafts featured greater decrease in fluorescein staining, greater superficial vascularization-free corneal area, lower variability of the corneal epithelial thickness, and higher corneal epithelial basal cell density. Autologous cultured LSC transplantation was associated with high long-term survival and dramatic improvement in vision and was very safe. Autologous limbal tissue transplantation resulted in similar efficiency but was less safe. Cadaver allogeneic grafts resulted in low long-term success rate and high prevalence of serious adverse events. STEM CELLS TRANSLATIONAL MEDICINE 2019;8:1230-1241 LESSONS LEARNED• Transplantation of autologous cultured limbal stem cells was associated with high long-term survival, dramatic improvement in vision, and high safety. • Transplantation of autologous limbal tissue resulted in similar efficiency but lower safety.• Cadaver allogeneic grafts resulted in low long-term success rate and high prevalence of serious adverse events. SIGNIFICANCE STATEMENTAutologous cultured limbal stem cell transplantation was associated with high long-term survival, vision improvement, and safety, whereas limbal grafts featured lower safety and allogeneic grafts featured low success rate and serious adverse events.

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Methods for Characterization/Manipulation of Human Corneal Stem Cells and their Applications in Regenerative Medicine

Cited by 15 publications

References 13 publications

Identification and characterization of dendritic cell subtypes in preserved and cultured cadaveric human corneolimbal tissue on amniotic membrane

Identification and characterization of dendritic cell subtypes in preserved and cultured cadaveric human corneolimbal tissue on amniotic membrane

Stem cells in Dentistry: knowledge and attitude of Nigerian Dentists

Long-Term Results of Cultured Limbal Stem Cell Versus Limbal Tissue Transplantation in Stage III Limbal Deficiency

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