Methods for Direct and Indirect Measurement of Mycoplasma Growth

Rodwell, A. W.; Whitcomb, R. F.

doi:10.1016/b978-0-12-583801-6.50036-6

Cited by 85 publications

(46 citation statements)

References 5 publications

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“…The quantification of mycoplasmas was adapted from Rodwell and Whitcomb [23]. Briefly, the M. arginini strain isolated as described above was subcultured in 100 mL of SP4 broth at 37°C in aerobiosis in culture flasks.…”

Section: Media and Reagentsmentioning

confidence: 99%

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Ribeiro‐Dias

Russo

Barbuto

et al. 1999

Journal of Leukocyte Biology

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Bacterial products stimulate macrophage tumoricidal activity through release of tumor necrosis factor (TNF) and nitric oxide (NO). We show here that thioglycollate-elicited macrophages acquire cytotoxic activity when cocultured with Mycoplasma arginini-infected YAC-1 tumor cells and release TNF and NO. Fixed mycoplasmainfected cells, supernatants from infected-cell cultures, or purified heat-killed mycoplasma obtained from cell-free cultures were all able to induce TNF and NO production. Thus, the mycoplasma per se and not a product of infected cells induce the release of these molecules. Addition of prostaglandin E 2 (PGE 2 ) to the cocultures, which reduced TNF release, or antibodies to TNF, did not affect macrophage cytotoxicity nor NO release. Inhibition of NO production by L-NAME or aminoguanidine reduced the cytotoxicity, and treatment with a NO donor was toxic to YAC-1 cells. These results indicate that M. arginini activates thioglycollateelicited murine macrophages for NO and TNF release increasing their cytotoxic activity toward YAC-1 cells and that this activity is dependent on NO but not TNF release. J. Leukoc. Biol. 65: 808-814; 1999.

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Section: Media and Reagentsmentioning

confidence: 99%

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Ribeiro‐Dias

Russo

Barbuto

et al. 1999

Journal of Leukocyte Biology

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“…An unfiltered sample was also examined as a control. Filtered and unfiltered samples were titrated by preparing serial 10-fold dilutions and then determining a viability count (CFUs) for each dilution (18). Sterol dependence.…”

Section: Methodsmentioning

confidence: 99%

Mycoplasma auris sp. nov., Mycoplasma cottewii sp. nov., and Mycoplasma yeatsii sp. nov., New Sterol-Requiring Mollicutes from the External Ear Canals of Goats

DaMassa

Tully

Rose

et al. 1994

International Journal of Systematic Bacteriology

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“…R strain is a virulent M. gallisepticum strain, which has been well characterized (Rodriguez & Kleven, 1980). The titre of each inoculum (expressed in colour changing units per millilitre) was determined as previously described (Rodwell & Whitcomb, 1983). The origins, characteristics and inoculation titres of the isolates used in this study are presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the egg transmission and pathogenicity of Mycoplasma gallisepticum isolates genotyped as ts-11

Armour

Ferguson‐Noel

2015

Avian Pathology

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Live Mycoplasma gallisepticum vaccines are used for the control of respiratory disease, egg production losses and egg transmission associated with M. gallisepticum infection in long-lived poultry. The first field case of apparent increased virulence and vertical transmission of ts-11, a live M. gallisepticum vaccine, has been reported. In that study a M. gallisepticum isolate from the broiler progeny of ts-11-vaccinated breeders was genotyped as ts-11 by sequence analysis of four different genetic targets and Random Amplified Polymorphic DNA and found to be significantly more virulent than ts-11 vaccine. The objective of the current study was to evaluate the rate of egg transmission and pathogenicity of ts-11 vaccine and isolates recovered from ts-11-vaccinated breeders (K6222B) and their broiler progeny (K6216D) which had been genotyped as ts-11. Groups of 28-week-old specific pathogenfree chickens at 87% average weekly egg production were inoculated with sterile broth media (negative controls), ts-11 vaccine, K6222B, K6216D or R strain (positive controls) by eye-drop and aerosol. K6216D transmitted via the egg at an average rate of 4.0% in the third and fourth weeks post-infection, while egg transmission of K6222B and ts-11 vaccine was not detected. M. gallisepticum was isolated from the air sacs, ovaries and oviducts of hens infected with K6216D and K6222B, but not from those infected with ts-11 vaccine. K6216D and K6222B both induced respiratory signs and significantly more tracheal colonization and more severe tracheal and air sac lesions than ts-11 vaccine (P ≤ 0.05). There were no substantial differences in the egg production of ts-11, K6216D and K6222B infected groups. These results provide the first conclusive evidence of transovarian transmission of an isolate genotyped as ts-11 and indicate that isolates genotyed as ts-11 vary in their virulence and ability to transmit via the egg.

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Methods for Direct and Indirect Measurement of Mycoplasma Growth

Cited by 85 publications

References 5 publications

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Mycoplasma auris sp. nov., Mycoplasma cottewii sp. nov., and Mycoplasma yeatsii sp. nov., New Sterol-Requiring Mollicutes from the External Ear Canals of Goats

Evaluation of the egg transmission and pathogenicity of Mycoplasma gallisepticum isolates genotyped as ts-11

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