Methods for enhancing the accuracy and reproducibility of Congo red and thioflavin T assays

Candida albicans Als Adhesins Have Conserved Amyloid-Forming Sequences

“…2B). These protein aggregates also enhanced the absorbance of Congo red, a characteristic used to define amyloids (6,22). Difference spectra illustrate this effect ( Fig.…”

“…The gelled peptide bound Congo red, enhancing the absorbance about twofold (characteristic of amyloids) and also redshifting the absorbance peak to 541 nm (Fig. 3A) (6). A similar sample of the peptide that had not been stirred did not significantly affect the Congo red spectrum.…”

“…Ten microliters of nonaggregated or aggregated peptide/protein sample was added, mixed well, and incubated at room temperature for 30 min before reanalysis (6,22).…”

Dark Field Microscopic Sensitive Detection of Amyloid Fibrils Using Gold Nanoparticles Modified with Antibody

“…5 Another advantage of this method is that fibrils can be distinguished from the monomer. Although Aβ samples can be sensitively detected with ELISA with a detection limit in the pM range, it cannot distinguish Aβ aggregates from Aβ monomer.…”

“…4 The amyloid fibrils were mainly detected with the help of amyloid specific probes, such as thioflavin T (ThT) and Congo red. 4,5 However, it has some drawbacks. First, sub-μM amounts of amyloid fibrils are necessary for the detection.…”