Methods for Improving <i>In Vitro</i> and <i>In Vivo</i> Boar Sperm Fertility

Funahashi, Hiroaki

doi:10.1111/rda.12568

Cited by 12 publications

(7 citation statements)

References 120 publications

(219 reference statements)

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“…Such a steady low efficiency has, together with experimental evidence of the effects of seminal plasma on the female, called for the application of SP during or after human embryo transfer [ 205 , 206 , 207 ]. Growing evidence has established SP playing major roles in embryo development and birth rates [ 102 ], and although the matter remains unsolved for humans, the experimental evidence from various animal models has quite nicely defined these effects on the female [ 12 , 93 , 120 ] ( Figure 5 ).…”

Section: What Proof Do We Really Have That Seminal Plasma Really Affects Fertility?mentioning

confidence: 99%

“…Under the procedures of IVF and/or sperm cryopreservation, the SP has even been considered detrimental and for decades customarily removed [ 8 , 9 ]. However, SP seems to warrant functions beyond fertilization, and is therefore related—in many species—with fertility [ 5 , 10 , 11 , 12 , 13 ]. Noteworthy, human SP has not been explored for eventual accompanying deterioration as what spermatozoa had been [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Seminal Plasma: Relevant for Fertility?

Rodriguez‐Martinez

Martı́nez

Calvete

et al. 2021

IJMS

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Seminal plasma (SP), the non-cellular component of semen, is a heterogeneous composite fluid built by secretions of the testis, the epididymis and the accessory sexual glands. Its composition, despite species-specific anatomical peculiarities, consistently contains inorganic ions, specific hormones, proteins and peptides, including cytokines and enzymes, cholesterol, DNA and RNA—the latter often protected within epididymis- or prostate-derived extracellular vesicles. It is beyond question that the SP participates in diverse aspects of sperm function pre-fertilization events. The SP also interacts with the various compartments of the tubular genital tract, triggering changes in gene function that prepares for an eventual successful pregnancy; thus, it ultimately modulates fertility. Despite these concepts, it is imperative to remember that SP-free spermatozoa (epididymal or washed ejaculated) are still fertile, so this review shall focus on the differences between the in vivo roles of the SP following semen deposition in the female and those regarding additions of SP on spermatozoa handled for artificial reproduction, including cryopreservation, from artificial insemination to in vitro fertilization. This review attempts, including our own results on model animal species, to critically summarize the current knowledge of the reproductive roles played by SP components, particularly in our own species, which is increasingly affected by infertility. The ultimate goal is to reconcile the delicate balance between the SP molecular concentration and their concerted effects after temporal exposure in vivo. We aim to appraise the functions of the SP components, their relevance as diagnostic biomarkers and their value as eventual additives to refine reproductive strategies, including biotechnologies, in livestock models and humans.

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Section: What Proof Do We Really Have That Seminal Plasma Really Affects Fertility?mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seminal Plasma: Relevant for Fertility?

Rodriguez‐Martinez

Martı́nez

Calvete

et al. 2021

IJMS

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“…Different species, as well as male individuals within the same species ( 34 ), have differences in substances and concentrations of these that can induce capacitation. Heparin, serum albumin, epinephrine, penicillamine, hypotaurine, coffein, bicarbonate, and calcium are some of the substances that can induce capacitation ( 35 , 36 ). It is quite obvious that there is a large step from maturing and fertilising oocytes in vitro to actually being able to produce live offspring after both procedures since this seems to take over 20 years in most species ( Table 1 ).…”

Section: History Of Ivp In Domestic Mammalsmentioning

confidence: 99%

In vitro fertilisation in domestic mammals—a brief overview

Sjunnesson

2019

Upsala Journal of Medical Sciences

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Many factors influence the final oocyte maturation, fertilisation, and early embryo development, and there are both similarities and differences between species. When comparing the advancement of assisted reproductive technologies (ARTs), the development in the bovine species is not far behind the medical front, with around one million in vitro-produced bovine embryos each year. This rate of progress is not seen in the other domestic species. This review aims to give an overview of the development and specific difficulties of in vitro embryo production in various domestic animal species, with the main focus on cows, pigs, and cats. In production animals, the aim of ARTs is commonly to increase the genetic progress, not to treat reproductive failure. The ARTs are also used for preservation of genetic diversity for the future. However, specifically for oocyte maturation, fertilisation, and early embryonic development, domestic mammals such as the cow and pig can be used as models for humans. This is particularly attractive from an animal welfare point of view since bovine and porcine oocytes are available in large numbers from discarded slaughterhouse material, thereby decreasing the need for research animals. Both for researchers on the animal and human medical fronts, we aim for the development of in vitro production systems that will produce embryos and offspring that are no different from those conceived and developed in vivo. Species-comparative research and development can provide us with crucial knowledge to achieve this aim and hopefully help us avoid unnecessary problems in the future. ARTICLE HISTORY

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“…Furthermore, techniques to produce embryos through in vitro maturation and fertilization (IVM-IVF) of the COCs derived from medium follicles (MF; with 3-6 mm in diameter) of pre-pubertal gilt have been well developed in the pig (Day & Funahashi, 1996;, whereas a high incidence of polyspermy is still remaining as a persistent obstacle (Funahashi, 2003(Funahashi, , 2015. However, the number of MF-derived COCs are very limited in an ovary, since there are mostly small follicles (SF), with 1-2 mm diameter (Morbeck, Esbenshade, Flowers, & Britt, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…Although the oocytes in cumulus–oocyte complexes (COCs) from pre‐pubertal gilts are known to have lower meiotic and cytoplasmic competences than those from cycling sows (Bagg, Nottle, Armstrong, & Grupen, ; Lechniak et al., ; Pawlak, Renska, Pers‐Kamczyc, Warzych, & Lechniak, ; Pawlak et al., ), most in vitro studies have used COCs derived from pre‐pubertal gilts, because these are the most common class of females slaughtered in many countries. Furthermore, techniques to produce embryos through in vitro maturation and fertilization (IVM‐IVF) of the COCs derived from medium follicles (MF; with 3–6 mm in diameter) of pre‐pubertal gilt have been well developed in the pig (Day & Funahashi, ; Funahashi & Day, ), whereas a high incidence of polyspermy is still remaining as a persistent obstacle (Funahashi, , ). However, the number of MF‐derived COCs are very limited in an ovary, since there are mostly small follicles (SF), with 1–2 mm diameter (Morbeck, Esbenshade, Flowers, & Britt, ).…”

Section: Introductionmentioning

confidence: 99%

Supplementation with cumulus cell masses improves the in vitro meiotic competence of porcine cumulus–oocytes complexes derived from small follicles

Matsunaga

Funahashi

2017

Reprod Domestic Animals

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The present study was conducted to examine the supplemented effect of cumulus cell masses (CCMs) derived from middle follicle (MF; 3-6 mm diameter) on the morphology and the meiotic or developmental competence of oocytes from small follicles (SF; 1-2 mm diameter). The number of cumulus cells surrounding oocytes just after collection was also lower in cumulus-oocyte complexes (COCs) from SF than MF. The ooplasmic diameter of oocytes was significantly smaller in SF-derived oocytes than MF-derived ones before and after in vitro maturation (IVM), whereas the diameter significantly increased during the culture. Co-culture of SF-derived COCs with MF-derived CCMs during IVM significantly improved the meiotic competence of the oocytes to the metaphase-II stage. Furthermore, the ooplasmic diameter of SF-derived COCs during IVM was increased to the similar size of MF-derived those in the presence of MF-derived CCMs. The abilities of oocytes to be penetrated, to form male pronuclear formation and to cleave or develop to the blastocyst stage were not affected by the co-culture with CCMs. Electrophoretic analysis of CCM secretions clearly showed the presence of more protein(s) approximately 27.6 kDa in the conditioned medium when supplemented with MF-derived CCMs. In conclusion, we demonstrate that supplementation with MF-derived CCMs improves the ooplasmic diameter and meiotic competence of SF-derived oocytes.

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Methods for Improving In Vitro and In Vivo Boar Sperm Fertility

Cited by 12 publications

References 120 publications

Seminal Plasma: Relevant for Fertility?

Seminal Plasma: Relevant for Fertility?

In vitro fertilisation in domestic mammals—a brief overview

Supplementation with cumulus cell masses improves the in vitro meiotic competence of porcine cumulus–oocytes complexes derived from small follicles

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