Methods for increasing the resolution of two-dimensional protein electrophoresis

Hochstrasser, Denis F.; Harrington, Michael G.; Hochstrasser, A.-C.; Miller, Mark J.; Merril, Carl R.

doi:10.1016/0003-2697(88)90209-6

Cited by 650 publications

(358 citation statements)

References 13 publications

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“…Isoelectric focusing was performed at a maximum voltage of 3,500 V, until a volt‫ء‬hour count of 35,000 was reached. Equilibration and transfer to the second dimension were done as described previously (26). SDS-PAGE for the second dimension was performed on 11% polyacrylamide gels.…”

Section: Two-dimesional Analysis Of Phosphorylated Bcl10mentioning

confidence: 99%

Bcl10 Controls TCR- and FcγR-Induced Actin Polymerization

Azagra

Gaide

Ho³

et al. 2007

The Journal of Immunology

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Bcl10 plays an essential role in the adaptive immune response, because Bcl10-deficient lymphocytes show impaired Ag receptor-induced NF-κB activation and cytokine production. Bcl10 is a phosphoprotein, but the physiological relevance of this posttranslational modification remains poorly defined. In this study, we report that Bcl10 is rapidly phosphorylated upon activation of human T cells by PMA/ionomycin- or anti-CD3 treatment, and identify Ser138 as a key residue necessary for Bcl10 phosphorylation. We also show that a phosphorylation-deficient Ser138/Ala mutant specifically inhibits TCR-induced actin polymerization yet does not affect NF-κB activation. Moreover, silencing of Bcl10, but not of caspase recruitment domain-containing MAGUK protein-1 (Carma1) induces a clear defect in TCR-induced F-actin formation, cell spreading, and conjugate formation. Remarkably, Bcl10 silencing also impairs FcγR-induced actin polymerization and phagocytosis in human monocytes. These results point to a key role of Bcl10 in F-actin-dependent immune responses of T cells and monocytes/macrophages.

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Section: Two-dimesional Analysis Of Phosphorylated Bcl10mentioning

confidence: 99%

Bcl10 Controls TCR- and FcγR-Induced Actin Polymerization

Azagra

Gaide

Ho³

et al. 2007

The Journal of Immunology

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“…Protein concentrations in the supernatant were determined using the Bradford method (1976). Two-dimensional gel electrophoresis was performed according to the O'Farrell method (Hochstrasser et al, 1988) with some modifications. A total of 80 g of protein was precipitated with 8 volumes of cold acetone.…”

Section: Two-dimensional Gel Electrophoresis and Protein Gel Blot Anamentioning

confidence: 99%

ADF Proteins Are Involved in the Control of Flowering and Regulate F-Actin Organization, Cell Expansion, and Organ Growth in Arabidopsis

Dong¹,

Xia²,

Yan³

et al. 2001

The Plant Cell

111

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“…Gels were stained with ammoniacal silver nitrate for analytical gels (Hochstrasser et al 1988) or Comassie staining for preparative gels. The gels were scanned by densitometer ImageScanner II (GE Healthcare Life Science, NJ, USA) and the ImageMaster 2D Platinum 6.0 software (GE Healthcare Life Science, NJ, USA) was used to perform the qualitative and quantitative analysis of the 2-DE maps.…”

Section: -De Image Acquisition and Analysismentioning

confidence: 99%

Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach

et al. 2009

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Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.

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Methods for increasing the resolution of two-dimensional protein electrophoresis

Cited by 650 publications

References 13 publications

Bcl10 Controls TCR- and FcγR-Induced Actin Polymerization

Bcl10 Controls TCR- and FcγR-Induced Actin Polymerization

ADF Proteins Are Involved in the Control of Flowering and Regulate F-Actin Organization, Cell Expansion, and Organ Growth in Arabidopsis

Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach

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