Methods for multiscale structural and functional analysis of the mammalian cochlea

Vogl, Christian; Neef, Jakob; Wichmann, Carolin

doi:10.1016/j.mcn.2022.103720

Cited by 4 publications

(3 citation statements)

References 173 publications

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“…The anatomical location of the auditory organ of the inner ear is deeply buried in the temporal side of the head, which makes it impossible to pursue the conventional treatment through surgical treatment, drug delivery, and tissue engineering technology 43,44 . Recently, kinds of organoid models are promising tools to advance the study of regenerative medicine, which validates the rapid development of new treatments of drug or gene‐based therapies 20 .…”

Section: Discussionmentioning

confidence: 99%

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Zhang,

Liu,

Shen

et al. 2024

J Biomed Mater Res

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Sensorineural hearing loss (SNHL) is mainly caused by injury or loss of hair cells (HCs) and associated spiral ganglion neurons (SGNs) in the inner ear. At present, there is still no effective treatment for SNHL in clinic. Recently, advances in organoid bring a promising prospect for research and treatment of SNHL. Meanwhile, three‐dimensional (3D) printing provides a tremendous opportunity to construct versatile organoids for tissue engineering and regenerative medicine. In this study, gelatin (Gel), sodium alginate (SA), and polyvinyl alcohol (PVA) were used to fabricate biomimetic scaffold through 3D printing. The organ of Corti derived from neonatal mice inner ear was seeded on the PVA/Gel/SA scaffold to construct organ of Corti organoid. Then, the organ of Corti organoid was used to study the potential protective effects of berberine sulfate on neomycin‐juried auditory HCs and SGNs. The results showed that the PVA/Gel/SA biomimetic 3D scaffolds had good cytocompatibilities and mechanical properties. The constructed organoid could maintain organ of Corti activity well in vitro. In addition, the injury intervention results showed that berberine sulfate could significantly inhibit neomycin‐induced HC and SGN damage. This study suggests that the fabricated organoid is highly biomimetic to the organ of Corti, which may provide an effective model for drug development, cell and gene therapy for SNHL.

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Section: Discussionmentioning

confidence: 99%

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Zhang,

Liu,

Shen

et al. 2024

J Biomed Mater Res

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“…We expect hair cell activity at positions with a resonant frequency close to that of the signal to decrease while having a minimal effect for positions further away. Directly testing this prediction would require measuring the response in a live cochlea, a difficult but perhaps feasible task with emerging optical coherence tomography techniques [34,35]. It might also be possible to indirectly test this prediction with simpler psychoacoustic methods.…”

Section: Discussionmentioning

confidence: 99%

Hair cells in the cochlea must tune resonant modes to the edge of instability without destabilizing collective modes

Momi,

Abbott,

Rubinfien

et al. 2024

Preprint

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Sound produces surface waves along the cochlea's basilar membrane. To achieve the ear's astonishing frequency resolution and sensitivity to faint sounds, dissipation in the cochlea must be canceled via active processes in hair cells, effectively bringing the cochlea to the edge of instability. But how can the cochlea be globally tuned to the edge of instability with only local feedback? To address this question, we use a discretized version of a standard model of basilar membrane dynamics, but with an explicit contribution from active processes in hair cells. Surprisingly, we find the basilar membrane supports two qualitatively distinct sets of modes: a continuum of localized modes and a small number of collective extended modes. Localized modes sharply peak at their resonant position and are largely uncoupled. As a result, they can be amplified almost independently from each other by local hair cells via feedback reminiscent of self-organized criticality. However, this amplification can destabilize the collective extended modes; avoiding such instabilities places limits on possible molecular mechanisms for active feedback in hair cells. Our work illuminates how and under what conditions individual hair cells can collectively create a critical cochlea.

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“…Temporal bone 3D microanatomy is undergoing a renewed interest thanks to the availability of novel X-ray-based and fluorescence-based nondestructive methods of imaging (11). Although both imaging approaches produce similar results, fluorescence-based methods based on tissue clearing (12) allow labeling of cell populations or vascular and matrix components, which is essential for understanding physiological and pathological processes as well as immune cell distribution and interactions (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Temporal bone marrow of the rat and its connections to the inner ear

Perin,

Cossellu,

Vivado

et al. 2024

Front. Neurol.

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Calvarial bone marrow has been found to be central in the brain immune response, being connected to the dura through channels which allow leukocyte trafficking. Temporal bone marrow is thought to play important roles in relation to the inner ear, but is still largely uncharacterized, given this bone complex anatomy. We characterized the geometry and connectivity of rat temporal bone marrow using lightsheet imaging of cleared samples and microCT. Bone marrow was identified in cleared tissue by cellular content (and in particular by the presence of megakaryocytes); since air-filled cavities are absent in rodents, marrow clusters could be recognized in microCT scans by their geometry. In cleared petrosal bone, autofluorescence allowed delineation of the otic capsule layers. Within the endochondral layer, bone marrow was observed in association to the cochlear base and vestibule, and to the cochlear apex. Cochlear apex endochondral marrow (CAEM) was a separated cluster from the remaining endochondral marrow, which was therefore defined as “vestibular endochondral marrow” (VEM). A much larger marrow island (petrosal non-endochondral marrow, PNEM) extended outside the otic capsule surrounding semicircular canal arms. PNEM was mainly connected to the dura, through bone channels similar to those of calvarial bone, and only a few channels were directed toward the canal periosteum. On the contrary, endochondral bone marrow was well connected to the labyrinth through vascular loops (directed to the spiral ligament for CAEM and to the bony labyrinth periosteum for VEM), and to dural sinuses. In addition, CAEM was also connected to the tensor tympani fossa of the middle ear and VEM to the endolymphatic sac. Endochondral marrow was made up of small lobules connected to each other and to other structures by channels lined by elongated macrophages, whereas PNEM displayed larger lobules connected by channels with a sparse macrophage population. Our data suggest that the rat inner ear is surrounded by bone marrow at the junctions with middle ear and brain, most likely with “customs” role, restricting pathogen spread; a second marrow network with different structural features is found within the endochondral bone layer of the otic capsule and may play different functional roles.

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Methods for multiscale structural and functional analysis of the mammalian cochlea

Cited by 4 publications

References 173 publications

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Hair cells in the cochlea must tune resonant modes to the edge of instability without destabilizing collective modes

Temporal bone marrow of the rat and its connections to the inner ear

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