Methods for producing a reproducible crush in the sciatic and tibial nerve of the rat and rapid and precise testing of return of sensory function

Koning, P. de; Brakkee, Jan H.; Gispen, Willem Hendrik

doi:10.1016/0022-510x(86)90109-7

Cited by 170 publications

(103 citation statements)

References 13 publications

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“…All fibers degenerate following the crush lesion and a nearly equal number of fibers regenerate (Legrain, 1977;Mira, 1977;De Koning, 1986). A test procedure employing local application of small electric currents was used to monitor recovery of sensory function following a crush lesion of the caudal nerves of the rat.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the method allows measuring and registering the process of regeneration from day 4 onwards, which certainly is an advantage as compared to other studies on peripheral regeneration (Bijlsma, 1983;De Koning, 1986). Also, most importantly, the repair process can be observed throughout a long period of time (if desired up to approximately 35 days).…”

Section: Discussionmentioning

confidence: 99%

“…In earlier studies using the sciatic crush method a positive effect of ORG.2766 on the recovery of sensory function following a crush lesion was found (Bijlsma, 1983: De Koning, 1986Verhaagen, 1986).…”

Section: Introductionmentioning

confidence: 94%

“…An incision was made in the middle of the ventral tendon sheet, exposing the major caudal nerve. The nerve was raised and crushed for 30 s, as described by De Koning et al (1986). A Kocher crush forceps with a waffle shaped inner side of the mouth was used.…”

Section: Surgerymentioning

confidence: 99%

“…There are, however, only few techniques available that allow monitoring of both these qualities longitudinally (D'Amour, 1941;Guttman, 1942;Legrain, 1977). Recently a useful method in this respect was described by De Koning et al (1986). Regeneration of the sciatic and tibial nerve after crush damage was measured by testing recovery of sensory function on the footsole of the rat at 4 different points.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A new approach for the evaluation of recovery after peripheral nerve damage

Hoop

Brakkee

Kapelle

et al. 1988

Journal of Neuroscience Methods

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The major caudal nerves of the rat provide an excellent model for longitudinal evaluation of nerve repair following a crush lesion. The surgical procedure and the method for testing sensory recovery are described in detail. Using this technique a clear, positive effect of ORG.2766 (an ACTH (4-9) analog) on the regeneration of sensory nerves could be shown. Results support the suggestion that ORG.2766 enhances the initial sprouting response, rather than exerting an effect on the growth rate of newly developed sprouts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Surgerymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A new approach for the evaluation of recovery after peripheral nerve damage

Hoop

Brakkee

Kapelle

et al. 1988

Journal of Neuroscience Methods

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Functional recovery from sciatic nerve crush lesion in the rat correlates with individual differences in responses to chronic intermittent stress

et al. 1997

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The aim of the present study was to monitor the influence of chronic stress on functional recovery from a sciatic nerve crush lesion in the rat. Male Wistar rats underwent standard unilateral sciatic nerve crush. Subsequently, chronic stress was induced during the recovery phase using a daily 30 min shock box session where rats received three electric footshocks each session (0.5 sec, 1 mA). Reduced body weight gain, adrenal gland hypertrophy, and thymus involution indicated that the stress rats were chronically stressed. Evaluation of sensorimotor function revealed significant differences in recovery between control and stress groups. Correlational analysis of individual stress rats indicated that recovery of the walking pattern was negatively correlated with adrenal gland and medulla enlargement, thymus involution, and plasma levels of adrenocorticotrophic hormone (ACTH) and corticosterone 45 min following the final stress session. In control rats, the index of sciatic nerve function (SF index, expressed as the difference between the injured paw and the intact contralateral paw as a percentage) was significantly correlated with adrenal medulla weight only. The present study reveals that chronic intermittent footshock stress impedes sensorimotor recovery following a sciatic nerve crush lesion and that the consequences of chronic intermittent stress are individually determined. We suggest that the quality of functional locomotor recovery after nerve crush lesion is related to the adaptive capacity or coping style of the individual rat.

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Electrophysiological evaluation of spinal reflexes during epidural anesthesia in an experimental model

et al. 1996

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We assessed the effects of epidural anesthesia with bupivacaine in the rat by serial recordings of spinal reflexes. The H wave from plantar muscles after electrical stimulation of the sciatic nerve evaluates a large nerve fiber spinal reflex arch. The extensor reflex response recorded from quadriceps muscle after stimulation of the contralateral tibial nerve assesses a reflex arch with small fiber afferents. After epidural injection of 0.2 mL of bupivacaine (0.25%, 0.5%, and 1.0% solutions) at the L5‐L6 vertebral space, nociceptive, H, and extensor reflex responses were abolished within 1–3 min. Duration of complete blockade lasted 20–80 min, increasing with the anesthetic concentration, and complete recovery occurred after an additional period of 30–40 min. The responses recovered to amplitudes similar to preanesthesia controls, indicating that there was no damage to the nervous system. This study shows that electrophysiological recording and quantitation of nerve reflex responses is a useful and accurate method to evaluate the efficacy of local anesthetic agents. © 1996 John Wiley & Sons, Inc.

show abstract

Methods for producing a reproducible crush in the sciatic and tibial nerve of the rat and rapid and precise testing of return of sensory function

Cited by 170 publications

References 13 publications

A new approach for the evaluation of recovery after peripheral nerve damage

A new approach for the evaluation of recovery after peripheral nerve damage

Functional recovery from sciatic nerve crush lesion in the rat correlates with individual differences in responses to chronic intermittent stress

Electrophysiological evaluation of spinal reflexes during epidural anesthesia in an experimental model

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