Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of &lt;em&gt;Drosophila guttifera&lt;/em&gt;

Fukutomi, Yuichi; Matsumoto, Keiji; Funayama, Noriko; Koshikawa, Shigeyuki

doi:10.3791/56935

Cited by 15 publications

(14 citation statements)

References 22 publications

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“…To produce “black wing” flies, flies' entire wings were painted with a black ink marker (Mckie, Zebra Co., Ltd.). All individuals were reared with standard cornmeal/sugar/yeast/agar food (Fukutomi et al., 2018) under a photoperiod of 12:12 (light:dark) at 25°C.…”

Section: Methodsmentioning

confidence: 99%

No evidence for contribution of sexually monomorphic wing pigmentation pattern to mate choice in Drosophila guttifera

Niida

Koshikawa

2021

Ethology

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In many animal groups, sexually dimorphic ornaments are thought to evolve by intraspecific competition or mate choice. Some researchers pointed out that sexually monomorphic ornaments could also evolve by mate choice by both sexes or either sex. Many species of fruit fly have sexually monomorphic wing pigmentation. However, involvement of their sexually monomorphic ornaments in mate choice has not been tested. We aimed to examine whether the sexually monomorphic polka‐dotted pattern on wings of Drosophila guttifera contributes to mate choice. Because D. guttifera does not mate in the dark condition at all and no courtship sound has been observed, some visual information is likely to be used in mating behaviour. We compared the number of matings between individuals with and without wings and found that presence of wings influenced mating behaviour in both sexes. We then compared the number of matings between individuals bearing replaced wings, one group bearing conspecific D. guttifera wings and another group bearing heterospecific D. melanogaster wings with no pigmentation pattern. An effect of conspecific/heterospecific wings was only detected in mate choice by females. Comparison between wild‐type and black‐painted wings revealed no evidence of a contribution of wing pigmentation pattern to mate choice in either sex.

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Section: Methodsmentioning

confidence: 99%

No evidence for contribution of sexually monomorphic wing pigmentation pattern to mate choice in Drosophila guttifera

Niida

Koshikawa

2021

Ethology

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“…These backcrosses aimed to unify the genetic backgrounds to improve the mapping efficiency in the transcriptome analyses. Flies were reared with standard cornmeal/sugar/yeast/agar food at 25 °C [52].…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis reveals wingless regulates neural development and signaling genes in the region of wing pigmentation of a polka‐dotted fruit fly

et al. 2020

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How evolutionary novelties have arisen is one of the central questions in evolutionary biology. Preexisting gene regulatory networks or signaling pathways have been shown to be co‐opted for building novel traits in several organisms. However, the structure of entire gene regulatory networks and evolutionary events of gene co‐option for emergence of a novel trait are poorly understood. In this study, to explore the genetic and molecular bases of the novel wing pigmentation pattern of a polka‐dotted fruit fly (Drosophila guttifera), we performed de novo genome sequencing and transcriptome analyses. As a result, we comprehensively identified the genes associated with the pigmentation pattern. Furthermore, we revealed that 151 of these associated genes were positively or negatively regulated by wingless, a master regulator of wing pigmentation. Genes for neural development, Wnt signaling, Dpp signaling, and effectors (such as enzymes) for melanin pigmentation were included among these 151 genes. None of the known regulatory genes that regulate pigmentation pattern formation in other fruit fly species were included. Our results suggest that the novel pigmentation pattern of a polka‐dotted fruit fly might have emerged through multistep co‐options of multiple gene regulatory networks, signaling pathways, and effector genes, rather than recruitment of one large gene circuit.

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“…To produce black wing flies, their entire wings were painted with a black paint marker (Mckie, Zebra Co., Ltd., Japan). All individuals were reared with standard cornmeal/sugar/yeast/agar food under a photoperiod of 12:12 (light:dark) at 25˚C (Fukutomi, Matsumoto, Funayama, & Koshikawa, 2018).…”

Section: Methodsmentioning

confidence: 99%

Sexually monomorphic wing pigmentation pattern does not contribute to mate choice inDrosophila guttifera

Niida

Koshikawa

2020

Preprint

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In many animal groups, sexually dimorphic ornaments are thought to be evolved by intraspecific competition or mate choice. Some researchers pointed out that sexually monomorphic ornaments could also be evolved by mate choice by both sexes or either sex. Many species of fruit fly have sexually monomorphic wing pigmentation. However, involvement of their sexually monomorphic ornaments in mate choice has not been tested.We aimed to examine whether the sexually monomorphic polka-dotted pattern on wings of Drosophila guttifera contributes to mate choice. Because D. guttifera does not mate in the dark condition at all and courtship sound was not observed, some visual information is likely to be used in mating behaviour. We compared the number of mates between individuals with and without wings, and found that presence of wings influenced mate choice in both sexes. We then compared the number of mates between individuals bearing replaced wings, one group for conspecific D. guttifera wings and another group for heterospecific D. melanogaster wings with no pigmentation pattern. The effect of conspecific/heterospecific wings was only detected in mate choice by females. By comparison between wild-type and black-painted wings, we found no evidence of contribution of wing pigmentation pattern to mate choice in either sex.

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Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of <em>Drosophila guttifera</em>

Cited by 15 publications

References 22 publications

No evidence for contribution of sexually monomorphic wing pigmentation pattern to mate choice in Drosophila guttifera

No evidence for contribution of sexually monomorphic wing pigmentation pattern to mate choice in Drosophila guttifera

Transcriptome analysis reveals wingless regulates neural development and signaling genes in the region of wing pigmentation of a polka‐dotted fruit fly

Sexually monomorphic wing pigmentation pattern does not contribute to mate choice inDrosophila guttifera

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