Methods for the Detection and Quantification of Quorum-Sensing Signals in Food Spoilage

Prathyusha, A. M. V. N.; Annavarapu, Harish; Bramhachari, Pallaval Veera

doi:10.1007/978-981-32-9409-7_16

Cited by 1 publication

(1 citation statement)

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“…These metabolites mediate synchronized expression or repression of genes that perform a variety of functions such as biofilm formation, antifungal production, and antibiotic production, among others (Padder et al 2018 ). Furthermore, quorum sensing has been shown to play a role in preventing food spoilage (Prathyusha et al 2019 ). Although quorum sensing has been mostly studied in bacteria, it is also well-studied in certain fungal genera such as Aspergillus , Candida and Saccharomyces (Padder et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Fungi associated with the potato taste defect in coffee beans from Rwanda

et al. 2022

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Background Potato taste defect (PTD) of coffee is characterized by a raw potato like smell that leads to a lower quality taste in the brewed coffee, and harms the commercial value of some East African coffees. Although several causes for PTD have been proposed, none of them have been confirmed. Recently, high throughput sequencing techniques and bioinformatic analysis have shown great potential for identifying putative causal agents of plant diseases. Toward the goal of determining the cause of PTD, we examined raw coffee beans from Rwanda exhibiting varying PTD scores using an Illumina-based sequence analysis of the fungal rRNA ITS region. Results Six fungal amplicon sequence variants (ASVs) with high relative abundances correlated with coffee taste scores. Four of these ASVs exhibited negative correlations – Aspergillus versicolor, Penicillium cinnamopurpureum, Talaromyces radicus, and Thermomyces lanuginosus – indicating that they might be causing PTD. Two of these fungi exhibited positive correlations – Kazachstania humilis and Clavispora lusitaniae – indicating that they might be inhibiting organisms that cause PTD. Conclusions This study addressed PTD causality from a new angle by examining fungi with high throughput sequencing. To our knowledge, this is the first study characterizing fungi associated with PTD, providing candidates for both causality and biocontrol.

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Section: Discussionmentioning

confidence: 99%