Methods for the detection of thermotolerant campylobacters in foods: results of an inter‐laboratory study

Scotter, Susan L.; Humphrey, T. J.; Henley, A.

doi:10.1111/j.1365-2672.1993.tb03009.x

Cited by 28 publications

(8 citation statements)

References 8 publications

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“…Although significant, there was evidence that the two methods deviated, with some samples having differences of up to 690 MPN/L. However, a single study by Scotter et al ( 1993 ), demonstrated that even with the use of three independent culture-based methods (two of which were international standards) on the same sample, inter-laboratory Campylobacter results correlated, at most, 42%. Thus, it can be assumed that the results of the sensitivity, specificity and diagnostic accuracy, achieved for AS/NZS during the current study, is indicative of the normal variation observed in culture-based studies.…”

Section: Discussionmentioning

confidence: 99%

Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method

et al. 2015

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Campylobacter is the leading agent of diarrheal disease worldwide. This study evaluates a novel culture-PCR hybrid (MPN-PCR) assay for the rapid enumeration of Campylobacter spp. from estuarine and wastewater systems. To first evaluate the current, culture-based, Australian standard, an inter-laboratory study was conducted on 69 subsampled water samples. The proposed Most-Probable Number (MPN)-PCR method was then evaluated, by analysing 147 estuarine samples collected over a 2 year period. Data for 14 different biological, hydrological and climatic parameters were also collated to identify pathogen-environment relationships and assess the potential for method specific bias. The results demonstrated that the intra-laboratory performance of the MPN-PCR was superior to that of AS/NZS (σ = 0.7912, P < 0.001; κ = 0.701, P < 0.001) with an overall diagnostic accuracy of ~94%. Furthermore, the analysis of both MPN-PCR and AS/NZS identified the potential for the introduction of method specific bias during assessment of the effects of environmental parameters on Campylobacter spp. numbers.

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Section: Discussionmentioning

confidence: 99%

Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method

et al. 2015

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“…However, the ability to recover injured cells was not assessed, although the author acknowledged that this warranted further investigation. Furthermore, this medium was incubated directly at 42 °C for 24 h, unlike the media used in the present study which were all pre‐incubated at 37 °C for 4 h, a technique shown to improve recovery (Humphrey 1986b, 1989; Scotter et al . 1993).…”

Section: Discussionmentioning

confidence: 99%

Comparison of three enrichment media for the isolation of Campylobacter spp. from foods

Baylis

MacPhee

Martin³

et al. 2000

J Appl Microbiol

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124

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Aim: This study compared the performance of three Campylobacter enrichment broths: Bolton broth (BB), Campylobacter Enrichment broth (CEB) and Preston broth (PB). Methods and Results: Pure cultures of target and competitor organisms, and naturallycontaminated food samples, were used to establish the performance of these media. In pure culture the PB supported the growth of the greatest number of strains of Campylobacter spp. but failed to inhibit some competitor organisms. The CEB showed the opposite result, inhibiting all 15 competitor organisms used but failing to support the growth of ®ve Campylobacter strains. By comparison, BB showed the best compromise between inhibition of competitors and growth of Campylobacter. Conclusions: Plates inoculated with BB and CEB food enrichments resulted in more Campylobacter growth than those inoculated with PB, which supported signi®cantly less typical growth (P 0Á001). The most common competitor organism isolated from PB was Escherichia coli, and Pseudomonas spp. were frequently isolated from BB and CEB. Both BB and CEB were better than PB for the isolation of Campylobacter from naturallycontaminated foods, although BB yielded more con®rmed Campylobacter growth than CEB. Signi®cance and Impact of the Study: This study highlighted differences in performance of media used to isolate Campylobacter spp. from foods.

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“…In a multilaboratory collaborative study, Scotter et al (1993) found that preenrichment of poultry skin samples with a gradual addition of antibiotics Downloaded by [Purdue University] at 14:28 13 April 2015 suppressed competing organisms resulting in an improved recovery of Campylobacter, as did a nonselective blood agar isolation medium used in combination with a membrane filtration technique. Murphy et al (2005) found that the extent to which C. jejuni survived acid stress at pH 4.5 was dependent on the identity of the broth used to culture and stress the cells.…”

Section: Isolation Of Campylobacters From Foodsmentioning

confidence: 98%

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Levin

2007

Food Biotechnology

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Campylobacter jejuni is considered to be the leading cause of enteric illness in the United States and other industrialized nations, causing mild to severe symptoms including serious infections of the extremities and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry, where the intestine is colonized resulting in healthy animals as carriers. Wildlife have long been considered an infectious reservoir for campylobacters because of their close association with and contamination of surface waters. This review deals with the areas of: phenotypic characteristics of C. jejuni and related human pathogenic species of Campylobacter, their ecological distribution, virulence factors, isolation of C. jejuni from foods, serotyping of Campylobacter isolates, bacteriophage typing, molecular methods of detecting and typing campylobacters, the viable but nonculturable state of campylobacters, the coccoid form of C. jejuni and immunomagnetic capture of C. jejuni.

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Methods for the detection of thermotolerant campylobacters in foods: results of an inter‐laboratory study

Cited by 28 publications

References 8 publications

Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method

Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method

Comparison of three enrichment media for the isolation of Campylobacter spp. from foods

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

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