Methods for the Determination of Thiamine

Mickelsen, Olaf; Yamamoto, R.

doi:10.1002/9780470110225.ch8

Cited by 30 publications

(3 citation statements)

References 208 publications

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“…The content of total phenols in root exudates was determined with the Folin-Ciocalteu method (Atanassova et al 2011), while the content of total flavonoids was determined following the Miliauskas et al (2004) protocol. Total flavonols were quantified according to the Yermakov method (Mickelsen and Yamamoto 1958), and chelating compounds were determined using a modified protocol of the Chrome Azurol S (CAS) method (Shenker et al 1995). Organic acids were determined by high-performance liquid chromatography (HPLC), as reported in Hullot et al (2021).…”

Section: Collection and Analysis Of Root Exudatesmentioning

confidence: 99%

Fire-induced effects on the bioavailability of potentially toxic elements in a polluted agricultural soil: implications for Cr uptake by durum wheat plants

Rascio

Gattullo

Porfido

et al. 2022

Environ Sci Pollut Res

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Fire events can modify the distribution and speciation of potentially toxic elements (PTEs) in soil, especially if they are associated to organic matter (OM). In fact, OM can undergo substantial structural modifications at high temperatures, up to the complete mineralization. The present study aims to investigate the changes of PTEs’ bioavailability to durum wheat (Triticum durum Desf.) plants after simulating fire events (up to 300 °C and 500 °C) in an agricultural soil polluted by Cr, Zn, Cu, and Pb. The PTEs’ uptake and allocation in plant tissues were assessed using the RHIZOtest system. After the fire simulations, no evident risk of accumulation and translocation in plants was observed for Zn, Pb, and Cu. Conversely, a high accumulation in roots and a significant translocation to shoots were observed for Cr, which reached concentrations of 829 mg kg−1 in roots and 52 mg kg−1 in shoots at 500 °C. Additional experimental evidence suggested that Cr was taken up by plants grown on heated soils as Cr(VI). Once acquired by roots, only a small part of Cr (up to 6%) was translocated to shoots where it was likely present as mobile forms, as evidenced by micro X-ray fluorescence (µ-XRF) analyses. Overall, the results obtained provide evidence that the high temperatures occurring during fire events can increase the mobility and bioavailability of certain PTEs transforming apparently safe environments into potentially dangerous sources of pollution. These processes can ultimately affect the human health through the food chain transfer of PTEs or their migration into surface water and groundwater.

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Section: Collection and Analysis Of Root Exudatesmentioning

confidence: 99%

Fire-induced effects on the bioavailability of potentially toxic elements in a polluted agricultural soil: implications for Cr uptake by durum wheat plants

Rascio

Gattullo

Porfido

et al. 2022

Environ Sci Pollut Res

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“…The content of total flavonols was determined following the Yermakov method (Mickelsen & Yamamoto 1958). Rutin hydrate (Sigma Aldrich, purity [?]…”

Section: Determination Of Total Flavonolsmentioning

confidence: 99%

Single and Combined Abiotic Stresses Impact the Root Exudation Patterns in Different Maize Root Types

Tiziani

Miras-Moreno

Malacrinò

et al. 2021

Preprint

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Root exudates play an essential role in plant-soil-abiotic stress interactions. However, we still know little about the influence of stress combinations on the root exudation profile. Using targeted and untargeted metabolomics, here we test the effect of drought, heat stress, and their combination on the maize root exudates, also considering the differences that might exist between root types (seminal and primary) and root zones (apical and sub-apical). In addition, we built an analytical framework that relate the root exudation profile with the characterization of the rhizosphere bacterial community, enabling us to dissect the interactions between specific root exudates and microbial taxa. Our results suggest that the composition of root exudates has a different outcome according to the single or combined stress and to the root zone but not between root types. Further, we found that stress-specific exudates influence the relative abundance of specific microbial taxa, some of which are known to be beneficial microorganisms. Therefore, the stress-specific root exudate composition selecting specific microbial taxa, here observed, represent a contribute on the effects of climate changes on crops increasing thus the potential impact on the current trend of crafting agricultural practices within a wider point of view of plants-microbe-environment interactions.

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“…There are different methods of measurement of thiamin content in foods and biological samples (urine, blood and other tissues), such as high‐performance liquid chromatography (HPLC) followed by fluorescence or ultraviolet detection, fluorimetry and microbiological assay (Icke and Nicol, ; Lynch and Young, ; Talwar et al., ; Mickelsen and Yamamoto, ). Techniques based on fluorimetric detection involve the oxidation of thiamin into thiochrome (Fayol, ).…”

Section: Definition/categorymentioning

confidence: 99%

Dietary reference values for thiamin

Turck¹,

Bresson²,

Burlingame³

et al. 2016

EFS2

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Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derived dietary reference values (DRVs) for thiamin (vitamin B1). The Panel considers that data from depletion-repletion studies in adults on the amount of dietary thiamin intake associated with the erythrocyte transketolase activity coefficient (aETK) < 1.15, generally considered to reflect an adequate thiamin status, or with the restoration of normal (baseline) erythrocyte transketolase activity, without a sharp increase in urinary thiamin excretion, can be used to estimate thiamin requirement. In the absence of new scientific evidence, the Panel endorses the average requirement (AR) of 0.072 mg/MJ (0.3 mg/1,000 kcal) for all adults proposed by the Scientific Committee for Food (SCF) in 1993 on the basis of one depletion-repletion study, in which both aETK and urinary thiamin excretion were measured. Results from other depletion-repletion studies are in agreement with this value. The Panel agrees on the coefficient of variation of 20% used by the SCF to cover uncertainties related to distribution of thiamin requirements in the general population, and endorses the population reference intake (PRI) of 0.1 mg/MJ (0.4 mg/1,000 kcal) set by the SCF for all adults. The same AR and PRI as for adults, expressed in mg/MJ, are proposed for infants aged 7-11 months, children aged 1 to < 18 years, and during pregnancy and lactation, under the assumption that the relationship between thiamin requirement and energy requirement is the same in all population groups. Acknowledgements: The Panel wishes to thank EFSA member of staff Fanny H eraud for the support provided to this scientific opinion.

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Methods for the Determination of Thiamine

Cited by 30 publications

References 208 publications

Fire-induced effects on the bioavailability of potentially toxic elements in a polluted agricultural soil: implications for Cr uptake by durum wheat plants

Fire-induced effects on the bioavailability of potentially toxic elements in a polluted agricultural soil: implications for Cr uptake by durum wheat plants

Single and Combined Abiotic Stresses Impact the Root Exudation Patterns in Different Maize Root Types

Dietary reference values for thiamin

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