Methods for the Quantification of Ethylene Produced by Plants

Bassi, Pawan K.; Spencer, Margaret Beale

doi:10.1007/978-3-642-83346-5_17

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…Vitality and biochemical parameters were measured as well: the membrane stability index in roots (MSIR) and/or shoots (MSIS) (Sairam et al 1997), root vitality test by the triphenyl-tetrazolium chloride (TTC) method (Clemensson-Lindell and Persson 1995) and the root ethylene production (ET) by GC (Bassi and Spencer 1989;Cristescu et al 2012).…”

Section: Measured Parametersmentioning

confidence: 99%

Selection of plant physiological parameters to detect stress effects in pot experiments using principal component analysis

et al. 2019

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Appropriate selection and well-timed measurement of plant developmental, morphological and physiological parameters are essential to maximize efficacy and minimize time consumption of experiments. To select for the most sensitive indicators of drought or salt stress, three independent pot experiments with diverse setups were analysed with 20-20 measured parameters. Parameters of plant growth, phenology and symbiotic interactions, visual stress symptoms, photosynthetic activity, nutrient composition and vitality were studied and the result matrices were evaluated with principal component analysis (PCA). Stress effects manifested in PC1 of two experiments and in PC2 of the third one. Traits assumed to be adequate for stress indication were characterized by high PC1 or PC2 loading values. Beside parameters of biomass production, growth and visible stress symptoms, less evident traits e.g. root electrical capacitance, membrane stability index in roots and leaves, relative water content of leaves and SPAD units were identified.

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Section: Measured Parametersmentioning

confidence: 99%

Selection of plant physiological parameters to detect stress effects in pot experiments using principal component analysis

et al. 2019

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“…The highly sensitive laser-driven photoacoustic detector in an intracavity set-up has created the possibility to measure these rapid changes in ethylene release. Such measurements would have been impossible with collection traps and traditional gas chromatographic TIME Is) analyses of ethylene because of insufficient sensitivity (see Bassi and Spencer, 1989). De-submergence of R. palustris results in a rapid release of ethylene; more than 90% of the entrapped ethylene is released within 1 min.…”

Section: Discussionmentioning

confidence: 99%

Rapid ethylene release and stimulated ethylene production after de-submergence of plants: An analysis with a continuous flow system in line with a laser-driven intracavity photoacoustic ethylene detector

Voesenek

Thier

Blom

et al. 1992

Progress in Plant Growth Regulation

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“…The major drawback of this approach based on thermal conductivity detection (TCD) was a relatively high detection limit of 10-100 μL/L. The introduction of flame ionisation detection (FID) and the photoionisation detector (PID) in 1980s significantly improved the detection limit of ethylene to tens of nL/L levels (Bassi and Spencer 1985;Bassi and Spencer 1989). At the beginning, the ethylene sampling procedure and its subsequent injection into the GC column have been done manually with a gas-tight syringe, which was filled with gas from the headspace of a closed cuvette, in which the plant was enclosed for a few hours (Abeles et al 1992).…”

Section: Ethylenementioning

confidence: 99%

Quo vadis plant hormone analysis?

Tarkowská

Novák

Floková

et al. 2014

Planta

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Plant hormones act as chemical messengers in the regulation of myriads of physiological processes that occur in plants. To date, nine groups of plant hormones have been identified and more will probably be discovered. Furthermore, members of each group may participate in the regulation of physiological responses in planta both alone and in concert with members of either the same group or other groups. The ideal way to study biochemical processes involving these signalling molecules is 'hormone profiling', i.e. quantification of not only the hormones themselves, but also their biosynthetic precursors and metabolites in plant tissues. However, this is highly challenging since trace amounts of all of these substances are present in highly complex plant matrices. Here, we review advances, current trends and future perspectives in the analysis of all currently known plant hormones and the associated problems of extracting them from plant tissues and separating them from the numerous potentially interfering compounds.

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Methods for the Quantification of Ethylene Produced by Plants

Cited by 12 publications

References 14 publications

Selection of plant physiological parameters to detect stress effects in pot experiments using principal component analysis

Selection of plant physiological parameters to detect stress effects in pot experiments using principal component analysis

Rapid ethylene release and stimulated ethylene production after de-submergence of plants: An analysis with a continuous flow system in line with a laser-driven intracavity photoacoustic ethylene detector

Quo vadis plant hormone analysis?

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