Methods for the Quantitation of Bromosulfophthalein and Its Glutathione Conjugate in Biological Fluids

Snel, Cor; Zhao, Ying; Mulder, G. J.; Pang, K. Sandy

doi:10.1006/abio.1993.1286

Cited by 18 publications

(10 citation statements)

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“…19,20 The formation of a GSH-DTNB conjugate was monitored by the change in absorbance at 412 nm. The determination was carried out according to the manufacturer's protocol (Jiancheng Bioengineering Ltd).…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Role of oxidative stress in elevated blood pressure induced by high free fatty acids

Wang

Hou

et al. 2009

Hypertens Res

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The aim of the study is to investigate the possible mechanism of oxidative stress in the high free fatty acids (FFAs)-induced hypertension. Male Sprague-Dawley rat models were established and classified into three groups, namely the control group (NC group), the FFA group, and the N-acetylcysteine (NAC) group. Blood pressure (BP) was recorded. An organ chamber experiment was performed to determine endothelium-dependent/-independent vasodilation (EDV/EIV). Reactive oxygen species (ROS), nitrotyrosine, reduced glutathione hormone (GSH) and NO 2 À /NO 3 À levels were measured in plasma. Endothelial nitric oxide synthase (eNOS) mRNA expression in endothelial cells was evaluated by real-time PCR. The following results were observed: (1) In the FFA group, BP increased after 4 h infusion of Intralipid+heparin. In the NAC group, systolic and diastolic BP remained the same. (2) In the FFA group, the aortic rings tended to show impaired EDV in response to acetylcholine (ACh). There was no difference of EDV response in the NAC and NC groups. (3) In the FFA group, NO 2 À /NO 3 À levels were significantly reduced, and eNOS mRNA expression and activity were significantly decreased compared with the NC group. NAC administration increased eNOS mRNA expression and activity. (4) ROS and nitrotyrosine concentrations in the FFA group were higher than in the NC group, and GSH concentrations in the FFA group were lower than in the NC group. Elevated FFAs can induce elevated BP, potentially through FFA-induced impairment of EDV resulting from decreased eNOS mRNA expression and activity. Oxidative stress may also play an important role in potential mechanisms of this high FFA-induced elevated BP.

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Section: Biochemical Measurementsmentioning

confidence: 99%

Role of oxidative stress in elevated blood pressure induced by high free fatty acids

Wang

Hou

et al. 2009

Hypertens Res

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“…BSP is metabolized by glutathione-S-transferase to bromosulfophthalein monoglutathione conjugate (BSP-mGSH) "(Wilco Snel et al, 1993). BSP is removed from blood and transported into liver cells where it is conjugated with glutathione and excreted into bile ( Van't Klooster et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…BSP is removed from blood and transported into liver cells where it is conjugated with glutathione and excreted into bile ( Van't Klooster et al, 1988). Glutathione appears to be the only conjugate of BSP (Combes, 1959;Wilco Snel et al, 1993). Once inside the bile canaliculi, BSP-mGSH may be hydrolyzed to BSP-monocysteinylglycine and other conjugated metabolites.…”

Section: Introductionmentioning

confidence: 99%

“…In male rats the 100 principle conjugate is BSP-mGSH (76%), which is followed by free BSP (14%), and BSP-mCys (4%) (Sano et al, 1992). The analysis of BSP and its conjugates from perfused liver and bile has been done via high-performance liquid chromatography (HPLC) (Wilco Snel et al, 1993). Many of these HPLC assays utilize the absorbance of BSP at 578 rn in alkaline pH ( Van't Klooster et al, 1988, Sano et al, 1992.…”

Section: Introductionmentioning

confidence: 99%

“…albumin are available (Baker and Bradley 1966;Pfaff et al 1975, Weisiger et al, 1984. The IPRL can be easily used to study factors important in BSP kinetics.…”

Section: Introductionmentioning

confidence: 99%

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1997 Toxic Hazards Research Annual Report

Dodd¹

1998

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Glutathione conjugation of bromosulfophthalein in relation to hepatic glutathione content in the rat in vivo and in the perfused rat liver

1995

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The relation between the rate of glutathione (GSH) conjugation and hepatic GSH content was studied in the rat in uiuo and the in situ single-pass-perfused rat liver preparation with bromosulfophthalein (BSP) as the model substrate. The biliary excretion of the BSP-GSH conjugate and the hepatic GSH content were monitored simultaneously during intravenous infusions with BSP in the rat in uiuo, and during liver perfusions with BSPcontaining perfusion medium. Rats were pretreated with single or multiple doses of buthionine sulfoximine, an inhibitor of the de nouo synthesis of GSH. Surprisingly, the excretion of the BSP-GSH conjugate was sustained at a high rate, despite a virtually complete depletion of hepatic GSH, both in the rat in uiuo as well as in the perfused rat liver. The results indicate that GSH was still available for conjugation with BSP after apparent depletion of the hepatic GSH pool, presumably because of a residual de nouo synthesis of GSH in the liver. Despite the multiple pretreatment with buthionine sulfoximine, the de nouo GSH synthesis was sufficient to sustain a high rate of GSH conjugation of BSP. The cosubstrate-K, for GSH conjugation of BSP in the liver was estimated to be very small (approximately 0.3 pmoVg): the excretion rate of the BSP-GSH conjugate was only impaired at minimal hepatic GSH levels. (HEPATOLOGY 1995;21:1387-1394 Glutathione (GSH) conjugation is an important biotransformation reaction of xenobiotics and endogenous compounds containing electrophilic groups. The reaction is catalyzed by the glutathione-S-transferases (Enzyme Committee 2.5.1.18) and requires the endogenous tripeptide GSH. We have studied the dependence of GSH conjugation on hepatic GSH in the rat in vivo Abbreviations: GSH, glutathione; BSP, bromosulfophthalein; BSO, D,Lbuthionine-[S,RI-sulfoximine; DEM, diethyl maleate; TLC, thin layer chromatography; HPLC. high-pressure liquid chromatography; v/v/v, volume to volume to volume; w/v, weight to volume.

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Methods for the Quantitation of Bromosulfophthalein and Its Glutathione Conjugate in Biological Fluids

Cited by 18 publications

References 0 publications

Role of oxidative stress in elevated blood pressure induced by high free fatty acids

Role of oxidative stress in elevated blood pressure induced by high free fatty acids

1997 Toxic Hazards Research Annual Report

Glutathione conjugation of bromosulfophthalein in relation to hepatic glutathione content in the rat in vivo and in the perfused rat liver

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