Methods for the quantitation of nitroglycerin and its metabolites in human plasma

Wu, Chien Chin; Sokoloski, Theodore D.; Burkman, Allan M.; Blanford, Marvin F.; Wu, Lei

doi:10.1016/s0378-4347(00)80450-8

Cited by 17 publications

References 15 publications

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Femtogram level quantitative determination of nitroglycerin and metabolites in human plasma by GC‐MS negative ion chemical ionization, single ion monitoring

Settlage¹,

Gielsdorf²,

Jaeger³

1983

J. High Resol. Chromatogr.

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SummaryRecent developments in the use of negative ion detection in GC-MS have indicated the potential for highlysensitiveanalyses for certain compounds. This paper describes the application of the techniques to the assay of Nitroglycerin and its metabolites yielding sensitivity to two femtograms. Becauseof thelow levels of nitroglycerin in biological media such sensitivity is highly desirable if not essential for meaningful measurements. In addition to sensitivity the method is shown to yield extremely good selectivity contributing further to the credibility of quantitative results.

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Femtogram level quantitative determination of nitroglycerin and metabolites in human plasma by GC‐MS negative ion chemical ionization, single ion monitoring

Settlage¹,

Gielsdorf²,

Jaeger³

1983

J. High Resol. Chromatogr.

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Simultaneous determination of glyceryl trinitrate and its metabolites in plasma using capillary gas chromatography with on‐column injection

Langseth-Manrique¹,

Bredesen²,

Greibrokk³

1986

J. High Resol. Chromatogr.

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A specific, sensitive, and precise assay for simultaneous quantitation of glyceryl trinitrate (GTN) and its metabolites 1,2and 1,3-glyceryl dinitrate (GDN) has been developed. The method involves one single extraction step with benzene, followed byevaporation at 55'C.One plof theextractwasinjected onto a capillary fused silica column with trifluoropropyl as the stationary phase.Thecolumnseparatedal1 thenitrateswithin 17 minutes using temperature programming from 90 to 155OC. The lower limits of detection were 0.05,0.5, and 0.1 nmol/l for GTN, 1,2-GDN and 1,3-GDN respectively. The calibration curves were linear in the concentration ranges 0.05-1 0.0 nmol/l (GTN), 0.5-10.0 nmol/l (1,2-GDN) and 0.1-10.0 nmol/l (1,3-GDN). Plasma concentration data from two patients receiving intravenous GTN infusion are presented.

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Determination of Picogram Nitroglycerin Plasma Concentrations Using Capillary Gas Chromatography with On-Column Injection

Noonan

Kanfer

Riegelman

et al. 1984

Journal of Pharmaceutical Sciences

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Methods for the quantitation of nitroglycerin and its metabolites in human plasma

Cited by 17 publications

References 15 publications

Femtogram level quantitative determination of nitroglycerin and metabolites in human plasma by GC‐MS negative ion chemical ionization, single ion monitoring

Femtogram level quantitative determination of nitroglycerin and metabolites in human plasma by GC‐MS negative ion chemical ionization, single ion monitoring

Simultaneous determination of glyceryl trinitrate and its metabolites in plasma using capillary gas chromatography with on‐column injection

Determination of Picogram Nitroglycerin Plasma Concentrations Using Capillary Gas Chromatography with On-Column Injection

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