Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development

Daszkowska‐Golec, Agata; Skubacz, Anna; Kurowska, Marzena; Slota, Michal; Swiergolik, Dorota; Szarejko, Iwona

doi:10.1007/978-1-4939-8944-7_9

Cited by 3 publications

(5 citation statements)

References 24 publications

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“…The answer seems to be related to the ABA dose since the response to ABA is highly dependent on the dose applied in terms of inhibition or stimulation of, e.g., growth [59]. The 200 µM applied in our study is rather a high concentration that allowed us to observe inhibition of growth by 60% in young barley seedling [30]. However, the application of such a high ABA concentration gave an interesting overview of changes in the photosynthetic apparatus.…”

Section: Discussionmentioning

confidence: 68%

“…Although subtle differences among cultivars studied were indicated, the tendency of alterations evoked by ABA was similar. We applied two ABA concentrations that provoked mild (10 µM) and strong (200 µM) reactions, respectively, during seedlings development in terms of growth inhibition as per our earlier studies [30]. The first applied ABA concentration (10 µM) did not reveal significant changes in OJIP curves, whereas the application of higher ABA concentration (200 µM) triggered a decrease of maximal fluorescence value (F M = P) in all of the genotypes tested.…”

Section: Exogenously Applied Abscisic Acid (Aba) Mostly Affected the mentioning

confidence: 99%

“…The experiment was performed according to the previously described work protocol [30]. Prior to the experiment, the seeds were surface-sterilized with 1% sodium hypochlorite for 20 minutes and then rinsed in water three times for 5 minutes.…”

Section: Aba Treatmentmentioning

confidence: 99%

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Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Daszkowska‐Golec

Collin

Sitko

et al. 2019

IJMS

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Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis.

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Section: Discussionmentioning

confidence: 68%

Section: Exogenously Applied Abscisic Acid (Aba) Mostly Affected the mentioning

confidence: 99%

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Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Daszkowska‐Golec

Collin

Sitko

et al. 2019

IJMS

Self Cite

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“…A half-strength Hoagland’s nutrient solution was used as the medium for the treatment with MeJA and pH was adjusted to 5.8. The composition of this solution was described elsewhere [ 122 ]. The seeds were surface sterilised by soaking in the 1% sodium hypochlorite for 15 min followed by rinsing them in sterile water three times for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

Kurowska

Daszkowska‐Golec

Gajecka

et al. 2020

IJMS

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Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2, HvTIP2;2, HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action.

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“…Homozygous T2 mutant lines, deletion mutants itpk1-2, itpk1-10, itpk1-22, and insertion mutants itpk1-33 and itpk1-27, were selected for in vivo testing of their response to salinity stress during germination according to the published protocol [79] with a few adjustments. Grains were sown on 0, 100 or 200 mM NaCl at 30 per plate in three replicates per treatment for each mutant line and WT.…”

Section: Germination Assaymentioning

confidence: 99%

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

Vlčko

Ohnoutková

2020

Plants

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Inositol trisphosphate 5/6 kinases (ITPK) constitute a small group of enzymes participating in the sequential phosphorylation of inositol phosphate to inositol hexakisphosphate (IP6), which is a major storage form of phosphate in cereal grains. The development of lines with reduced IP6 content could enhance phosphate and mineral bioavailability. Moreover, plant ITPKs participate in abiotic stress signaling. To elucidate the role of HvITPK1 in IP6 synthesis and stress signaling, a barley itpk1 mutant was created using programmable nuclease Cas9. Homozygous single bp insertion and deletion mutant lines were obtained. The mutants contained altered levels of phosphate in the mature grains, ranging from 65% to 174% of the wild type (WT) content. Homozygous mutant lines were tested for their response to salinity during germination. Interestingly, insertion mutant lines revealed a higher tolerance to salinity stress than deletion mutants. Mature embryos of an insertion mutant itpk1-2 and deletion mutant itpk1-33 were cultivated in vitro on MS medium supplemented with NaCl at 50, 100, and 200 mM. While both mutants grew less well than WT on no or low salt concentrations, the itpk1-2 mutant was affected less than the WT and itpk33 when grown on the highest NaCl concentration. The expression of all ITPKs was induced in roots in response to salt stress. In shoots, the differential effect of high salt on IPTK expression in the two iptk1 mutants was consistent with their different sensitivities to salt stress. The results extend the evidence for the involvement of ITPK genes in phosphate storage and abiotic stress signaling.

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Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development

Cited by 3 publications

References 24 publications

Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

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