Methods for the Study of Ticks, Mosquitoes, and their Transmitted Pathogens: Toward a Greater Understanding of Vector Biology and Arthropod-Microbe Interactions

Cull, Benjamin; Wang, Xinru

doi:10.3791/64986

Cited by 1 publication

(1 citation statement)

References 10 publications

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“…While infecting ticks via a viremic host is most similar to how they become infected in nature, these differences in viremia can confound potential differences in tick susceptibility to different virus strains and isolates because ticks acquire varying amounts of infectious virus during feeding. Therefore, ex vivo midgut and salivary gland cultures, backless tick models, and artificial membrane feeding could be utilized to better understand how genetic differences between POWV strains impact various aspect of infection and replication within tick tissues and cells [ 45 , 46 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Strain-Dependent Assessment of Powassan Virus Transmission to Ixodes scapularis Ticks

McMinn,

Gallichotte,

Courtney

et al. 2024

Viruses

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Powassan virus (POWV) is an emerging tick-borne encephalitic virus in Lyme disease-endemic sites in North America. Due to range expansion and local intensification of blacklegged tick vector (Ixodes scapularis) populations in the northeastern and upper midwestern U.S., human encephalitis cases are increasingly being reported. A better understanding of the transmission cycle between POWV and ticks is required in order to better predict and understand their public health burden. Recent phylogeographic analyses of POWV have identified geographical structuring, with well-defined northeastern and midwestern clades of the lineage II subtype. The extent that geographic and genetically defined sublineages differ in their ability to infect and be transmitted by blacklegged ticks is unclear. Accordingly, we determined whether there are strain-dependent differences in the transmission of POWV to ticks at multiple life stages. Five recent, low-passage POWV isolates were used to measure aspects of vector competence, using viremic and artificial infection methods. Infection rates in experimental ticks remained consistent between all five isolates tested, resulting in a 12–20% infection rate and some differences in viral load. We confirm that these differences are likely not due to differences in host viremia. Our results demonstrate that blacklegged ticks are susceptible to, and capable of transmitting, all tested strains and suggest that the tick–virus association is stable across diverse viral genotypes.

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Section: Discussionmentioning

confidence: 99%

Strain-Dependent Assessment of Powassan Virus Transmission to Ixodes scapularis Ticks

McMinn,

Gallichotte,

Courtney

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

Methods for the Study of Ticks, Mosquitoes, and their Transmitted Pathogens: Toward a Greater Understanding of Vector Biology and Arthropod-Microbe Interactions

Abstract: Huang, D. et al. Mosquito-associated virus isolation from field-collected mosquitoes.

Cited by 1 publication

References 10 publications

Strain-Dependent Assessment of Powassan Virus Transmission to Ixodes scapularis Ticks

Strain-Dependent Assessment of Powassan Virus Transmission to Ixodes scapularis Ticks

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