Methods for the treatment of collagenous tissues for bioprostheses

Khor, Eugene

doi:10.1016/s0142-9612(96)00106-8

Cited by 387 publications

(297 citation statements)

References 72 publications

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“…Gelatin has been selected because it is a partially denatured product of collagen (basement membrane of ECM of adult pancreatic islets) and contains Arg-Gly-Asp (R-G-D) sequence that help in binding of the integrins such as α5β1 and α5β3 of adult pancreatic islets and ensure its intact structure. 18 Gelatin has also known to be less antigenic when compared to collagen 19 and induces vasculature under in vivo conditions, the weak mechanical strength limits its use in pancreatic tissue engineering applications. The mechanical strength of hydrogels can be improved by preparing it as interpenetrating polymer network (IPN), which is defined as a physical mixture of at least two polymers that have been synthesized or cross linked in the presence of each other with no significant degree of covalent bonds between them.…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Muthyala

Bhonde²,

Nair³

2010

Islets

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Section: Discussionmentioning

confidence: 99%

Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Muthyala

Bhonde²,

Nair³

2010

Islets

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“…Collagen carriers, prepared by complete demineralization of bovine trabecular or cortical bone particles, have been shown to meet these requirements in animals (123,124). In clinical practice collagen has been used for more than 25 years in the replacement of ligaments, tendons and other soft tissues and as wound dressings, where it has proven its clinical safety (125,126). However, because of the possible risks of pathogen transmission of allogenic or xenogenic materials, the search for synthetic carriers continues.…”

Section: Role Of Bmp Carriers In Bone Inductionmentioning

confidence: 99%

Bone morphogenetic proteins in human bone regeneration

Groeneveld

Burgér

2000

European Journal of Endocrinology

358

217

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Recently, the first clinical reports on bone regeneration by two recombinant human bone morphogenetic proteins (rhBMPs), BMP-2 and BMP-7 (also named osteogenic protein-1, OP-1) have been published (1-4) . Although both BMPs were able to support bone regeneration, a significant variation in individual response was observed with both proteins. Animal studies and laboratory experiments reveal a number of conditions that influence the osteoinductivity of BMP, such as BMP concentration, carrier properties and influence of local and systemic growth factors and hormones. In this paper, these studies and the clinical reports are reviewed, and the conditions that modulate the BMP-dependent osteoinduction are discussed. The information may provide clues as to how the performance of recombinant human BMP as bone-graft substitute in humans can be improved. European Journal of Endocrinology 142 9-21 BMP and demineralized bone matrixThe history of bone morphogenetic proteins (BMPs) began with the observation that demineralized bone matrix (DBM) is able to induce ectopic bone formation in subcutaneous and intramuscular pockets in rodents (5, 6). This bone induction process has been studied extensively (7-13). Histological and biochemical analyses showed that cartilage appears 5-10 days after implantation of active DBM (8). This cartilage mineralizes by day 7-14 and is subsequently replaced by bone (7-9). After 21 days, haematopoietic bone marrow formation can be observed (12). These cellular events observed after DBM implantation mimic embryonic bone development and normal fracture repair (11). As DBM-related bone formation was observed to occur at ectopic sites, it was assumed that pluripotent mesenchymal cells are attracted to the site of implantation. Isolation of the bone-inducing substance revealed that certain proteins were responsible, which were termed bone morphogenetic proteins (BMPs) or osteogenetic proteins (OPs).The use of DBM in treating bone defects has proven beneficial for bone regeneration both in animals and in humans (14-17). DBM has become widely accepted as a bone-graft substitute in clinical practice (18-22), but its bone inductive capacity has been questioned (23, 24). In several studies, including our own, histology revealed that new bone was generated by osteoconduction rather than osteoinduction (25-27) -that is, bone regeneration occurred by growth of existing host bone on the DBM granules, which acted as a scaffold, rather than by de novo differentiation of bone, independent of pre-existing bone. Lack of inductive properties of DBM may be related to the procedures of production of commercially available DBM, as preservation of osteoinductive activity can be affected by the processing (28-30) or sterilization procedures (31). It is also possible that DBM of human origin, which is preferred for use in clinical practice, is less osteoinductive than DBM derived from animals, which is commonly used in animal studies. Several studies show that DBM from long-lived species such as baboon and human...

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“…Por esta razón, se hace necesario preservar su estructura originaria y su integridad mecánica así como eliminar, o al menos neutralizar, sus propiedades antigénicas. Los métodos para el tratamiento de tejidos biológicos se basan principalmente en la creación de enlaces entre las moléculas de colágeno, en concreto entre los grupos funcionales de los aminoácidos [36].…”

Section: Figura 4 Direcciones Anatómicas En El Pericardio [35]unclassified

“…Los métodos físicos son la aplicación de calor, el secado y la radiación con rayos ultravioleta. Entre los tratamientos químicos se encuentran el tratamiento con glutaraldehído, formaldehido [36], diimidoésteres, acilazida [37], componentes epoxy y cianamida. Comparando los distintos métodos de entrecruzamiento, se ha observado que todos tienen sus ventajas e inconvenientes.…”

Section: Figura 4 Direcciones Anatómicas En El Pericardio [35]unclassified

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Caracterización mecánica de materiales de base colágeno para aplicaciones cardiovasculares

Arnedo¹,

Almudena²

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Methods for the treatment of collagenous tissues for bioprostheses

Cited by 387 publications

References 72 publications

Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Bone morphogenetic proteins in human bone regeneration

Caracterización mecánica de materiales de base colágeno para aplicaciones cardiovasculares

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