Methods in Mechanical Testing of Arterial Tissue: A Review

Macrae, Ryley A.; Miller, Karol; Doyle, Barry

doi:10.1111/str.12183

Cited by 58 publications

(43 citation statements)

References 177 publications

(271 reference statements)

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“…It should be noted that a uniaxial ring test only provides preliminary insight into mechanical property changes caused by decellularization. A uniaxial test does not reflect the loading applied to blood vessels in vivo [54], and in any case cannot be used to identify mechanical properties of an elastic, anisotropic material [55]. To identify constitutive properties of these constructs and provide a framework for comparison of mechanical properties, biaxial planar testing of flat specimens or inflation-extension of cylindrical specimens is required [56].…”

Section: Hybrid Sds/scco 2 Decellularizationmentioning

confidence: 99%

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Casali

Handleton

Shazly

et al. 2018

The Journal of Supercritical Fluids

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A B S T R A C TDecellularized tissues are commonly utilized as tissue engineering scaffolds. Decellularization by extended exposure to aqueous detergents can damage the microstructure or deposit cytotoxic residue. Supercritical carbon dioxide (scCO 2 ) has been proposed for decellularization, but reportedly causes dehydration and scaffold embrittlement.Presented herein is a novel decellularization method that preserves matrix hydration state and mechanical properties. Over 97% of the water in porcine aorta is maintained by presaturating scCO 2 with water; however, complete decellularization was not attained by any process utilizing only scCO 2 . Instead, a novel hybrid method is presented that combines a brief (48 h) exposure of tissue to aqueous detergent, followed by washing with scCO 2 (1 h). The hybrid method fully decellularized the tissue, as confirmed by histology and DNA quantification (<0.04 μg DNA/mg tissue). This hybrid treatment was faster than the standard method (2 days compared to 4-7 days), while preserving tissue structure and mechanical properties.

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Section: Hybrid Sds/scco 2 Decellularizationmentioning

confidence: 99%

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Casali

Handleton

Shazly

et al. 2018

The Journal of Supercritical Fluids

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“…A healthy artery wall is composed of several layers: tunica intima, tunica media and tunica adventitia [20]. Tunica intima is the innermost layer, which is in direct contact with the blood.…”

Section: Constitutive Fiber-reinforced Materials Model For Artery Wallmentioning

confidence: 99%

Numerical evaluation of systematic errors of a non-invasive intracranial pressure measurement

Misiulis¹,

Skarbalius²,

Džiugys³

2018

energetika

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Intracranial pressure (ICP) monitoring procedure can be applied to aid in secondary brain damage prevention. A high invasiveness of commonly used ICP measuring methods poses a risk of complications, and therefore new non-invasive methods are currently being developed. A promising non-invasive ICP measurement method is based on the existence of pressure balance state, which is driven by the unique morphological property of ophthalmic artery (OA). The value of ICP can be obtained by evaluating blood flow or artery characteristics in different OA segments, intracranial OA segment (IOA) and extracranial OA segment (EOA). In order to increase measurement accuracy, the systematic errors must be evaluated, which requires an implementation of a numerical model encompassing various physical phenomena. In this paper, a developed numerical model is presented, which was used to solve a transient fluid–structure interaction (FSI) problem of the pulsatile blood flow in a straight, physically meaningful anisotropic, hyperelastic OA, with ICP and external pressure (Pe) loads. It was found that the systematic error based on mean cross-sectional area difference between IOA and EOA segments was {–1.48, –1.37, –1.17} mmHg with ICP = {10, 20, 30} mmHg, respectively. The systematic error based on mean blood flow velocity difference between IOA and EOA segments was {–1.84, –1.76, –1.625} mmHg with ICP = {10, 20, 30} mmHg, respectively. The presented numerical model examined the worst-case scenario in terms of boundary conditions, which were immovable, while lengths of OA segments were physiologically relevant statistical means; however, the obtained systematic errors still met the clinical standards of ANSI/AAMI, where it is stated that the error should not exceed the ± 2 mmHg in the range of 0–20 mmHg of ICP. Boundary conditions and compliance affects the systematic error in both ways (reduce or increase it); this may explain the low systematic errors obtained in experimental studies by other authors.

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“…The mechanical properties of the explanted graft were compared to adjacent carotid artery tissue and unseeded PEUU scaffold material. The force-extension curves were converted to stress-strain curves using the following expressions (Macrae et al, 2016):…”

Section: Mechanical Characterizationmentioning

confidence: 99%

Development of a Semi-Automated, Bulk Seeding Device for Large Animal Model Implantation of Tissue Engineered Vascular Grafts

Cunnane

Lorentz

Soletti

et al. 2020

Front. Bioeng. Biotechnol.

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Methods in Mechanical Testing of Arterial Tissue: A Review

Cited by 58 publications

References 177 publications

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Numerical evaluation of systematic errors of a non-invasive intracranial pressure measurement

Development of a Semi-Automated, Bulk Seeding Device for Large Animal Model Implantation of Tissue Engineered Vascular Grafts

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