Methods in melissopalynology: colour determination of pollen pellets for colour vision deficient individuals

Hornby, Sarah; Benn, Jonty; Vinkenoog, Rinke; Goldberg, Shannon; Pound, Matthew J.

doi:10.1080/01916122.2022.2062476

Cited by 6 publications

(8 citation statements)

References 37 publications

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“…Non-invasive and non-destructive sampling matrices, such as the APIStrips [10] or silicone wristbands [11], can be inserted into the hives to collect environmental pollutants. Hornby et al [12] developed a standardised way of identifying colours in pollen samples using a smartphone, which also enables participation of colour-blind people.…”

Section: Pos(acsc2022)015mentioning

confidence: 99%

Honey bees and beekeepers as environmental researchers: Results, limits, opportunities

Brodschneider¹,

Gratzer²

2023

Proceedings of Austrian Citizen Science Conference 2022 — PoS(ACSC2022)

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Beekeepers are more and more involved in studies on honey bees and the environment. With their special equipment and knowledge, they can participate in scientific studies by providing data or samples. Several such investigations have resulted in scientific publications and innovations have been made to offer beekeepers a low-threshold participation. These innovations include noninvasive sampling matrices and methods to analyse samples taken by beekeepers. The involvement of beekeepers also includes some limits, most of which are typical for environmental studies or other studies involving citizens, like sample logistics or data protection issues.

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Section: Pos(acsc2022)015mentioning

confidence: 99%

Honey bees and beekeepers as environmental researchers: Results, limits, opportunities

Brodschneider¹,

Gratzer²

2023

Proceedings of Austrian Citizen Science Conference 2022 — PoS(ACSC2022)

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“…A diverse selection of floral sources is required for a colony to get all their nutritional needs (Roulston and Cane 2000). Whilst the general rule still persists that honey bees forage on one plant per foraging trip, multiple studies have shown that around 40% of pollen pellets contain two forage sources (Betts 1935;Brodschneider et al 2018;Hornby et al 2022). Within all pellets, are typically small quantities of other pollen.…”

Section: Introductionmentioning

confidence: 99%

Determining if honey bees (Apis mellifera) collect pollen from anemophilous plants in the UK

et al. 2022

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Whether insect pollinators use wind-pollinated plants have implications for insect monitoring and conservation strategies in a wide range of environments. Habitats, such as coniferous plantations and arable crops of the Poaceae family are not typically considered priority for the monitoring of insect pollinators or habitat enhancement. Further many pollinator monitoring techniques focus on flowers and do not count insect interactions with wind-pollinated plants. Using two honey bee colonies from distinct environments (urban and rural) in north east England, we investigate the use of wind-pollinated plants over the summer of 2021. We combine honey bee pollen pellet analysis with airborne pollen sampling to investigate whether honey bees use three common wind-pollinated plant groups (Pinus sp., Plantago sp. and Poaceae) that have previously been considered sources of forage. Our results show that honey bees do forage on Plantago and Poaceae pollen, in line with previous studies. However, we show statistically that Pinus pollen is contamination from the atmosphere and not actively collected. It is important to consider airborne contamination before making interpretations based on small amounts of pollen in samples of bee products. The use of members of the Poaceae has implications for insect pollinator monitoring in urban environments, which has not always been considered in past studies.

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“…The pollen present in this bee product is a natural marker that can indicate which flowers are parts of their diet (Vieira et al 2020). The honey bee (Apis mellifera) uses plant pollen as an important nutritional source for amino acids, fats, minerals, proteins, starch, sterol and vitamins; where the nutritional quality of pollen pellets is dependent on the botanical origin of the pollen (Hornby et al 2022). The botanical origin covers the dominant plant source from which the honey is produced, while the geographical origin refers to the region from which the product is collected (da Luz et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Pollen Characterization of Polyfloral Honeys from Laghouat Region (Algeria)

2022

IJAB

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Honey is naturally produced by honeybees from the nectar of plants. It is widely consumed as a health food product all over the world. Melissopalynology plays an important role in ascertaining the botanical and geographical origins of honey by studying the pollen contained in the honey. The pollen content of 19 honey samples of Apis mellifera L. from the Laghouat region of Algeria was analyzed. 100 pollen types belong to 49 families were identified, of which the most diverse were Asteraceae (12 pollen types), Fabaceae (9), Apiaceae, Boraginaceae and Lamiaceae (5 pollen types each). Asteraceae and Fabaceae were present in all the samples, while, Apiaceae Nitrariaceae, Euphorbiaceae, Brassicaceae, and Oleaceae were identified in more than 84% of analysed honey samples. The number of pollen types identified per sample ranged between 13 and 27 (mean of 19.1). All honey samples were classified as a polyfloral honey. The best represented secondary pollen types were Peganum harmala, Centaurea sp., Trifolium sp., Eucalyptus sp., Euphorbia sp., Olea europaea, Echium sp., Onopordum arenarium, Lotus type and Cistus sp. For the quantitative analysis, the pollen content of the studied honey samples ranged from medium (class II, 57.9% of the samples) to high (class III, 42.1 % of the samples), where the pollen density ranged from 23 400 to 351 250 in 10 g of honey, with an average of 108 002 grains /10 g.

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Methods in melissopalynology: colour determination of pollen pellets for colour vision deficient individuals

Cited by 6 publications

References 37 publications

Honey bees and beekeepers as environmental researchers: Results, limits, opportunities

Honey bees and beekeepers as environmental researchers: Results, limits, opportunities

Determining if honey bees (Apis mellifera) collect pollen from anemophilous plants in the UK

Pollen Characterization of Polyfloral Honeys from Laghouat Region (Algeria)

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