Methods, interpretations, and different views of aspirations

Olson, Paul R.

doi:10.1007/bf01811021

Cited by 2 publications

(1 citation statement)

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“…Carbon monoxide (CO)-bound Mb (MbCO) has been frequently used as a model system for elucidating the ligand binding properties in detail, since MbCO is more stable than oxygenated Mb (MbO 2 ) [3,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35]. The CO stretching (ν CO ) frequency of MbCO can be observed by infrared (IR) spectroscopy, and its frequency is a sensitive probe of the distal pocket environment [36,37].…”

Section: Introductionmentioning

confidence: 99%

Carbon monoxide binding properties of domain-swapped dimeric myoglobin

et al. 2015

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Myoglobin (Mb) is a monomeric oxygen storage hemoprotein, and has been shown to form a domain-swapped dimer. In this study, monomeric and dimeric carbon monoxide (CO)-bound Mb (MbCO) exhibited similar absorption spectra. The CO stretching frequencies of MbCO were observed at 1,932 and 1,944 cm(-1) for both monomeric and dimeric MbCO. The resonance Raman (RR) bands for the stretching between the heme iron and axial ligands were observed at the same frequencies for the monomer and dimer of deoxygenated Mb (deoxyMb) and MbCO, respectively (ν Fe-His, 220 cm(-1); ν Fe-C, 507 cm(-1)), showing that the Fe-His bond strength of deoxyMb and the Fe-CO bond strength of MbCO did not change by the dimerization. Time-resolved RR measurements showed that the dynamics of the structural changes at the heme active site after CO photo-dissociation of MbCO was similar between monomeric and dimeric Mb [monomer, (5.2 ± 1.8) × 10(6) s(-1); dimer, (6.2 ± 1.1) × 10(6) s(-1) at room temperature]. These results show that the heme coordination structure, the protein environment around the bound CO, and the protein relaxation character are similar between monomeric and dimeric MbCO. Although the active site structure was similar between the monomer and dimer, the CO binding rate constant of dimeric Mb [(1.01 ± 0.03) × 10(6) M(-1) s(-1) at 20 °C] was about twice larger than that of the monomer [(0.52 ± 0.02) × 10(6) M(-1) s(-1) at 20 °C], presumably due to the expansion of the channel between the Xe3 cavity and the solvent by the dimerization.

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Section: Introductionmentioning

confidence: 99%

Carbon monoxide binding properties of domain-swapped dimeric myoglobin

et al. 2015

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Crossing the Great Divide – Using Multi‐method Analysis to Understand Opposition to Windfarms

Haggett

Toke

2006

Public Administration

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This paper demonstrates the possibilities for the complementary use of regression analysis and discourse analysis to further understand issues in public administration. To do so, an empirical study of opposition to wind energy planning applications is used. The application of logistical regression to analyse the factors which may influence windfarm planning applications is discussed, factors including the attitudes of local people. Discourse analytical techniques are then used to consider how anti-windfarm campaigners manage accusations of 'Not In My Back Yard' (NIMBYism). This is done partly by linking their cause with wider environmental objectives. Although discourses and logistical regression models have very different ontologies, the paper demonstrates that there is no inevitable conflict between the epistemologies used in these two different methods, despite differences in the type of data being analysed. INTRODUCTION: THE USE OF MULTI-METHODSThis paper demonstrates the possibilities for the complementary use of two methodologies -logistical regression analysis and discourse analysis -to tackle the same issue. The empirical example chosen to demonstrate this is opposition to windfarm planning applications in England and Wales. Logistical regression analysis is used to understand both the factors which influence the outcome of applications and the emergence of opposition. A particular variant of discourse analysis is used to understand how anti-windfarm campaigners justify their stance. These two techniques, one quantitative and one qualitative, are very different. However, we believe an epistemology can be deployed which allows their complementary use. As part of this process we therefore discuss what truth claims may be appropriate for such usage.In deploying such an epistemology, we place this research within existing debates on both methods and theory in public administration. In terms of method, there are ongoing discussions about the suitability of certain methods and of combining different methods. Lan and Anders (2000), for example, discuss the extent to which public administration research is inherently multi-disciplinary. They note that:

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Methods, interpretations, and different views of aspirations

Cited by 2 publications

References 5 publications

Carbon monoxide binding properties of domain-swapped dimeric myoglobin

Carbon monoxide binding properties of domain-swapped dimeric myoglobin

Crossing the Great Divide – Using Multi‐method Analysis to Understand Opposition to Windfarms

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