Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and a polymer epoxy resin surface at two hydrodynamic conditions and compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells when compared to glass (-1.27×105 cells.cm−2). Likewise, the number of adhered cells was significantly lower (-1.16×105 cells.cm−2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll α content, and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p <0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p <0.001). These findings demonstrated the high potential of initial adhesion assays to estimate marine biofilm development.