Methods of Inducing Inflammatory Bowel Disease in Mice

Abstract

Animal models of experimentally induced inflammatory bowel disease (IBD) are useful for understanding more about the mechanistic basis of disease, identifying new targets for therapeutic intervention, and testing novel therapeutic agents. This unit provides detailed protocols for four of the most commonly used mouse models of experimentally induced intestinal inflammation: chemical induction of colitis by dextran sodium sulfate (DSS), hapten-induced colitis via 2,4,6-trinitrobenzene sulfonic acid (TNBS), Helicobacter-induced colitis in mdr1a(-/-) mice, and the CD4(+) CD45RB(hi) SCID transfer colitis model.

Regulatory T-cell depletion in the gut caused by integrin β7 deficiency exacerbates DSS colitis by evoking aberrant innate immunity

“…The disease activity index and histological damage were assessed by trained individuals blinded to the treatment groups, as reported previously. 41 On day 10, mice were killed and colons removed and analyzed. For interim analyses, experiments were done as described above but terminated on day 4.…”

The Anti-atherosclerotic Dipeptide, Trp-His, Reduces Intestinal Inflammation through the Blockade of L-Type Ca2+ Channels

“…Mice were inspected daily for stool consistency, presence of blood in stool, or general appearance. The clinical scoring was calculated as described by Maxwell et al 23 and defined as follows: stool score, 0 = normal, 1 = moist/sticky stool, 2 = soft stool, 3 = diarrhea; stool blood score, 0 = no blood, 1 = evidence of blood in stool or around anus, 2 = severe bleeding; mouse appearance, 0 = normal, 1 = ruffled fur or altered gait, 2 = lethargic or moribund.…”

γ-Glutamyl cysteine and γ-glutamyl valine inhibit TNF-α signaling in intestinal epithelial cells and reduce inflammation in a mouse model of colitis via allosteric activation of the calcium-sensing receptor

“…Mice were monitored daily for stool consistency, presence of blood in stool or bleeding and general appearance, and a clinical activity score (ranging from 0 to 7) was calculated as described by Maxwell et al [24].…”

“…Paraffin-embedded sections of distal colon were stained with hematoxylin and eosin (H&E) (Animal Health Laboratory, University of Guelph, Guelph, Ontario), and histological scoring to assess colonic tissue injury and inflammation was performed as described by Maxwell et al [24].…”