Methods of Specific Diagnosis of Tuberculosis: A Modern View on the Problem

Prylutskyi, O S; Прилуцкий, А С; Rogovaya, YuD D; Роговая, Ю Д

doi:10.35693/2500-1388-2017-0-2-44-51

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Интерпретация результатов в разных государствах различна. В большинстве стран мира проба Манту считается положительной при наличии инфильтрата ≥10 мм у детей, не имеющих факторов риска, и ≥5 мм у детей, относящихся к группам риска [31][32][33][34]. В США у детей с высокой степенью риска заболеваемости туберкулезом (ВИЧ-инфицированные или дети, находящиеся в тесном контакте с больными туберкулезом) положительной считается проба Манту размером ≥5 мм.…”

Section: иммунологические тесты используемые в диагностике туберкулез...unclassified

“…Следует обратить внимание на то, что при выраженных иммунодефицитах (ВИЧ-инфекция, лечение иммунодепрессантами и др.) могут наблюдаться ложноотрицательные результаты реакции Манту [31]. В связи с вышеуказанными факторами диагностическая эффективность пробы Манту достигает всего 57,2% [36].…”

Section: иммунологические тесты используемые в диагностике туберкулез...unclassified

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Immunodiagnosis of Tuberculosis: Advantages and Disadvantages

Будрицкий¹,

Серёгина²,

Правада³

et al. 2023

Клиническая Инфектология И Паразитология

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Одним из приоритетных направлений в борьбе с туберкулезом является своевременная и достоверная диагностика туберкулеза легких. Для диагностики туберкулеза легких на современном этапе широко используются иммунологические методы. Оценка результатов пробы Манту и кожной пробы с препаратом Диаскинтест (АТР – аллерген туберкулезный рекомбинантный) носит субъективный характер, а также различается в разных странах и поэтому не всегда может быть достоверным критерием установления диагноза туберкулеза. Иммунодиагностика туберкулеза стала более информативной на современном этапе благодаря разработке и внедрению иммунологических тестов нового поколения IGRA (Interferon Gamma Release Assay). IGRA-тесты автоматизированы, и поэтому их результаты более объективны. Эти тесты позволяют выявить активные и латентные формы туберкулеза и характеризуются высокой специфичностью и чувствительностью. Все методы иммунологической диагностики, используемые на современном этапе, имеют как преимущества, так и недостатки. Поэтому они могут использоваться в зависимости от цели и возможностей лечебного учреждения и обязательно должны интерпретироваться в комплексе с другими диагностическими методами. One of the priorities in the fight against tuberculosis is the timely and reliable diagnosis of pulmonary tuberculosis. For the diagnosis of pulmonary tuberculosis at the present stage, immunological methods are widely used. Evaluation of the results of the Mantoux test and skin test with the drug Diaskintest (ATP) is subjective, and also varies in different countries and therefore may not always be a reliable criterion for establishing a diagnosis of tuberculosis. The immunodiagnosis of tuberculosis has become more informative at the present stage due to the development and implementation of a new generation of immunological tests IGRA (Interferon Gamma Release Assay). IGRA-tests are automated and therefore their results are more objective. These tests can detect active and latent forms of tuberculosis and are characterized by high specificity and sensitivity. All methods of immunological diagnostics used at the present stage have both advantages and disadvantages. Therefore, they can be used depending on the purpose and capabilities of the medical institution and must be interpreted in conjunction with other diagnostic methods.

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Section: иммунологические тесты используемые в диагностике туберкулез...unclassified

Immunodiagnosis of Tuberculosis: Advantages and Disadvantages

Будрицкий¹,

Серёгина²,

Правада³

et al. 2023

Клиническая Инфектология И Паразитология

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“…It is an important health care problem with significant economic and social consequences [1][2][3]. In modern phthisiology, a major role is assigned to the etiological diagnosis of TB, which means identifying the causative TB agent and determining its characteristics (drug susceptibility spectrum, species) [3][4][5][6]. These data are crucial for diagnosing, timely initiation of chemotherapy, choosing the correct treatment plan, monitoring an ongoing therapy, as well as implementing preventive measures in foci of TB infection [4,6].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2021

Saratov Med J

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Objective -to compare the efficacy of various molecular genetic methods for diagnosing tuberculosis and determining drug susceptibility to rifampicin (RIF). Materials and Methods. We conducted a retrospective study of the sputum analysis results on 1,992 patients with pulmonary tuberculosis treated at Saratov Oblast Clinical Tuberculosis (TB) Dispensary from 2014 through 2018. The following methods were used: real-time polymerase chain reaction (PCR), biological microarrays, automated Xpert® MTB/RIF technology. Statistical processing of the research results was carried out using the Bayes formula based on contingency tables (four-field table) and the χ2 test. When evaluating the significance of differences between relative values, we employed the critical significance level of 0.05. Results. In terms of etiological diagnosis of TB, higher diagnostic sensitivities of the real-time PCR and biological microarray methods (73.9% and 70.3%, correspondingly) were established, as compared with the Xpert® MTB/RIF method (34.2%) (p<0.001). The sensitivity of all methods depended on the massiveness of bacterial excretion and clinical form of TB. Conclusions: The Xpert MTB/RIF method exhibited lower diagnostic sensitivity in verifying the diagnosis of TB, whereas its operational characteristics in terms of determining RIF-resistance were sufficiently high (sensitivity at 89.7%, specificity at 89.1%, and efficacy at 89.4%), which was comparable with the characteristics of biological microarray method (93.9%, 71.8%, 82.9%; p=0.127, p<0.001, p=0.139, respectively).

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Comparative efficiency of various molecular genetic methods in diagnosing tuberculosis

Салина

Morozova

2021

Saratov Med J

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Objective – to compare the efficacy of various molecular genetic methods for diagnosing tuberculosis and determining drug susceptibility to rifampicin (RIF). Materials and Methods. We conducted a retrospective study of the sputum analysis results on 1,992 patients with pulmonary tuberculosis treated at Saratov Oblast Clinical Tuberculosis (TB) Dispensary from 2014 through 2018. The following methods were used: real-time polymerase chain reaction (PCR), biological microarrays, automated Xpert® MTB/RIF technology. Statistical processing of the research results was carried out using the Bayes formula based on contingency tables (four-field table) and the χ2 test. When evaluating the significance of differences between relative values, we employed the critical significance level of 0.05. Results. In terms of etiological diagnosis of TB, higher diagnostic sensitivities of the real-time PCR and biological microarray methods (73.9% and 70.3%, correspondingly) were established, as compared with the Xpert® MTB/RIF method (34.2%) (p<0.001). The sensitivity of all methods depended on the massiveness of bacterial excretion and clinical form of TB. Conclusions: The Xpert MTB/RIF method exhibited lower diagnostic sensitivity in verifying the diagnosis of TB, whereas its operational characteristics in terms of determining RIF-resistance were sufficiently high (sensitivity at 89.7%, specificity at 89.1%, and efficacy at 89.4%), which was comparable with the characteristics of biological microarray method (93.9%, 71.8%, 82.9%; p=0.127, p<0.001, p=0.139, respectively).

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Methods of Specific Diagnosis of Tuberculosis: A Modern View on the Problem

Cited by 4 publications

References 32 publications

Immunodiagnosis of Tuberculosis: Advantages and Disadvantages

Immunodiagnosis of Tuberculosis: Advantages and Disadvantages

Untitled

Comparative efficiency of various molecular genetic methods in diagnosing tuberculosis

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