Methods of studying soil microbial diversity

Kirk, Jennifer L.; Beaudette, Lee A.; Hart, Miranda M.; Moutoglis, Peter; Klironomos, John N.; Lee, Hung; Trevors, J. T.

doi:10.1016/j.mimet.2004.04.006

Cited by 716 publications

(434 citation statements)

References 125 publications

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“…Wang et al 2010). Soil microbial communities are responsible for various biogeochemical cycles and play pivotal roles in the cycling of organic compounds (Kirk et al 2004). Larger populations of microbial communities implied the more active biogeochemical cycles beneath Leucaena leucocephala (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Effects of vegetation restoration types on soil quality in Yuanmou dry-hot valley, China

Peng

Chen

Fang

et al. 2013

Soil Science and Plant Nutrition

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Soil erosion has resulted in serious land degradation in Yuanmou dry-hot valley, Yunnan province, China. Vegetation restoration had been widely developed in this hot and arid area. In this work we investigate the quality of rhizosphere soils under four restoration species in the towns of Zuolin (gully bed) and Yuanma (slope area). [pH, soil organic matter, nitrogen (N), phosphorus (P) and potassium (K) contents], and microbial (microbial biomass and microbe quantity) properties and soil enzyme (catalase, protease and urease) activities were determined. Compared to the soils collected from other vegetation types, the soil under Leucaena leucocephala had the highest content of soil organic matter, total N, available N, total P, available P, available K, microbial biomass carbon (C), and the highest microbe quantity and enzyme activities. Five parameters (organic matter, pH, microbial biomass N, total K and protease activity), which had the greatest weight in the principal components analysis, were selected to calculate the soil quality index (SQI). The final SQI values implied that restoring Leucaena leucocephala greatly improved the soil quality, while restoration with Eucalyptus camaldulensis decreased the soil quality of the slope area. Plant recovery with Dodonaea viscosa and Heteropogon contortus on the gully bed affected the population of soil microbial communities but didn't improve the SQI values. It can therefore be seen that types of plants used for recovery in gully-developed regions in dry-hot valleys must be chosen cautiously: Leucaena leucocephala is a good choice for soil revegetation of the slope area, but the revegetation patterns on the gully bed still need further study.

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Section: Discussionmentioning

confidence: 99%

Effects of vegetation restoration types on soil quality in Yuanmou dry-hot valley, China

Peng

Chen

Fang

et al. 2013

Soil Science and Plant Nutrition

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“…Extraction and amplification of a DNA fragment from soil samples by PCR, employing universal or specific primers, followed by different fingerprinting methods like DGGE/TGGE, T-RFLP, ARDRA, RISA (Kirk et al, 2004) or by cloning and sequencing, can be used to analyze the microbial community in time and space.…”

Section: Microbiological and Biochemical Indicatorsmentioning

confidence: 99%

Soil health: looking for suitable indicators. What should be considered to assess the effects of use and management on soil health?

Cardoso¹,

Vasconcellos²,

Bini³

et al. 2013

Sci. agric. (Piracicaba, Braz.)

418

220

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Soil Health refers to the ecological equilibrium and the functionality of a soil and its capacity to maintain a well balanced ecosystem with high biodiversity above and below surface, and productivity. To understand and use soil health as a tool for sustainability, physical, chemical, and biological properties must be employed to verify which respond to the soil use and management within a desired timescale. Attributes with a rapid response to natural or anthropogenic actions are considered good indicators of soil health. Among the physical indicators, soil texture, aggregation, moisture, porosity, and bulk density have been used, while among chemical indicators total C and N, mineral nutrients, organic matter, cation exchange capacity, among others are well established. However, most of them generally have a slow response, when compared to the biological ones, such as microbial biomass C and N, biodiversity, soil enzymes, soil respiration, etc., in addition to macro and mesofauna. Thus, a systemic approach based on different kinds of indicators (physical, chemical and biological) in assessing soil health would be safer than using only one kind of attribute. Many human activities have caused desertification, loss of biodiversity, disruption of aggregates, loss of organic matter and nutrients, among others. Today, it is imperious to maintain soil health and productivity with increasing emphasis on reforestation and recuperation of degraded areas through the use of organic amendments, reintroduction of plants, soil fauna and microorganisms. This review focused on an integrative view on indicators of soil health to be used as tools for prediction of sustainability in production systems.

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“…Phospholipid fatty acid profiles provide information on the viable microorganism and insight into the structure of the microbial community. However, individual fatty acid cannot be used to represent specific species (a single microorganism can have numerous fatty acids and the same fatty acids can occur in more than one species) (Kirk et al, 2004). PLFA is a microbial community profiling tool that produces profiles of limited complexity, thus PLFA is often used for conjunction with other molecular profiling methods to assess microbial diversity in soil and water (Jin and Kelly, 2007).…”

Section: Ratios Of Characteristic Fatty Acidmentioning

confidence: 99%

Comparative study of microbial community structure in different filter media of constructed wetland

Zhou

Tao

et al. 2010

Journal of Environmental Sciences

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Methods of studying soil microbial diversity

Cited by 716 publications

References 125 publications

Effects of vegetation restoration types on soil quality in Yuanmou dry-hot valley, China

Effects of vegetation restoration types on soil quality in Yuanmou dry-hot valley, China

Soil health: looking for suitable indicators. What should be considered to assess the effects of use and management on soil health?

Comparative study of microbial community structure in different filter media of constructed wetland

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