Methods to analyze extracellular vesicles at single particle level

Kwon, Yongmin; Park, Jaesung

doi:10.1186/s40486-022-00156-5

Cited by 27 publications

(19 citation statements)

References 96 publications

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“…Similar to NTA, DLS is also a well-established technique based on the Brownian motion of suspended particles that is commonly used for the analysis of miEVs size distribution and surface charge . Using this method, researchers found that infection of bovines with leukemia virus has no remarkable effect on isolated miEVs size distribution .…”

Section: Characterization Of Mievsmentioning

confidence: 99%

Milk-Derived Extracellular Vesicles: Biomedical Applications, Current Challenges, and Future Perspectives

Salehi,

Negahdari,

Mehryab

et al. 2024

J. Agric. Food Chem.

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Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that are released by almost all animal and plant cells and act as intercellular communicators by transferring their cargos between the source and target cells. As a safe and scalable alternative to conditioned medium-derived EVs, milk-derived EVs (miEVs) have recently gained a great deal of popularity. Numerous studies have shown that miEVs have intrinsic therapeutic actions that can treat diseases and enhance human health. Additionally, they can be used as natural drug carriers and novel classes of biomarkers. However, due to the complexity of the milk, the successful translation of miEVs from benchtop to bedside still faces several unfilled gaps, especially a lack of standardized protocols for the isolation of high-purity miEVs. In this work, by comprehensively reviewing the bovine miEVs studies, we provide an overview of current knowledge and research on miEVs while highlighting their challenges and enormous promise as a novel class of theranostics. It is hoped that this study will pave the way for clinical applications of miEVs by addressing their challenges and opportunities.

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Section: Characterization Of Mievsmentioning

confidence: 99%

Milk-Derived Extracellular Vesicles: Biomedical Applications, Current Challenges, and Future Perspectives

Salehi,

Negahdari,

Mehryab

et al. 2024

J. Agric. Food Chem.

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“…To more sensitively, precisely, and quantitatively detect, monitor, and investigate cancer EVs, EVs need to be analyzed at the single vesicle level. Different single vesicle technologies have been reported in recent years [ 204 ]. We expect nanomaterials will play a rapidly increasing role in the development of new SVTs for EV protein and nucleic acid detection and profiling, for basic vesicle research and clinical applications.…”

Section: Summary and Perspectivementioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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“…27 Recent technological advances have also demonstrated EV-based diagnosis for cancer, 28–30 Alzheimer's disease 31 and Parkinson's disease 32 with superior detection sensitivity to conventional testing methods. Due to their nanoscale sizes (∼50 nm–1 μm), 33 EV isolation remains a major bottleneck and researchers are actively developing novel tools and assays to study EV biology and improve their clinical adoption.…”

Section: Introductionmentioning

confidence: 99%