2017
DOI: 10.1371/journal.pone.0179251
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Methods to maximise recovery of environmental DNA from water samples

Abstract: The environmental DNA (eDNA) method is a detection technique that is rapidly gaining credibility as a sensitive tool useful in the surveillance and monitoring of invasive and threatened species. Because eDNA analysis often deals with small quantities of short and degraded DNA fragments, methods that maximize eDNA recovery are required to increase detectability. In this study, we performed experiments at different stages of the eDNA analysis to show which combinations of methods give the best recovery rate for … Show more

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Cited by 237 publications
(242 citation statements)
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“…New studies should take advantage of the increasing number of methodological papers being published on eDNA collection techniques, extraction techniques, and the statistical analysis of eDNA data (e.g., Lacoursière-Roussel, Rosabal, et al, 2016;Hinlo et al, 2017;Chambert, Takahara, Pilliod, Goldberg, & Doi, 2018) to optimize and standardize field, laboratory, and analytical practices. New studies should take advantage of the increasing number of methodological papers being published on eDNA collection techniques, extraction techniques, and the statistical analysis of eDNA data (e.g., Lacoursière-Roussel, Rosabal, et al, 2016;Hinlo et al, 2017;Chambert, Takahara, Pilliod, Goldberg, & Doi, 2018) to optimize and standardize field, laboratory, and analytical practices.…”
Section: Future Directionsmentioning
confidence: 99%
“…New studies should take advantage of the increasing number of methodological papers being published on eDNA collection techniques, extraction techniques, and the statistical analysis of eDNA data (e.g., Lacoursière-Roussel, Rosabal, et al, 2016;Hinlo et al, 2017;Chambert, Takahara, Pilliod, Goldberg, & Doi, 2018) to optimize and standardize field, laboratory, and analytical practices. New studies should take advantage of the increasing number of methodological papers being published on eDNA collection techniques, extraction techniques, and the statistical analysis of eDNA data (e.g., Lacoursière-Roussel, Rosabal, et al, 2016;Hinlo et al, 2017;Chambert, Takahara, Pilliod, Goldberg, & Doi, 2018) to optimize and standardize field, laboratory, and analytical practices.…”
Section: Future Directionsmentioning
confidence: 99%
“…Several recent studies have determined that filtration of larger water volumes (≥1 L) results in greater fish eDNA capture and detection than precipitation of eDNA from small water volumes (Piggott ; Hinlo et al. ; Spens et al. ).…”
Section: Volume Of Water Sampled In the Fieldmentioning
confidence: 99%
“…In our study, the probability of detection by qPCR was c. 4-6 times higher with a large water volume (750 ml) compared to a smaller volume (15 ml). Yet, using the same laboratory and field protocols as for the 750 ml samples, would have likely the resulted in even lower eDNA concentrations (Hinlo et al, 2017;Rees et al, 2014). In addition, the 15 ml samples were stored and processed differently from the larger volume which could have been affected the results.…”
Section: Discussionmentioning
confidence: 99%
“…Filtration of 250 ml water volumes has been the recommended minimum volume of water to produce successful detection in a range of aquatic species(Dougherty et al, 2017;Goldberg et al, 2016;Hinlo, Gleeson, Lintermans, & Furlan, 2017;Olds et al, 2016). Water samples of 250 ml were collected in triplicate from 10 evenly distributed sampling points per pond.…”
mentioning
confidence: 99%
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