Methotrexate-Albumin and Aminopterin-Albumin Effectively Prevent Experimental Acute Graft-versus-Host Disease

Abstract

The albumin conjugates MTX-HSA and AMPT-HSA effectively prevent experimental aGVHD.

The Role of Animal Models in the Study of Hematopoietic Stem Cell Transplantation and GvHD: A Historical Overview

“…A few years later, the discovery of calcineurin inhibitors (e.g., cyclosporine A and tacrolimus) greatly improved the prophylaxis protocols. When used early after transplantation, these drugs, alone or in combination with MTX successfully reduced GvHD occurrence in animal models (64, 74, 75, 120–122), and were soon after introduced to the clinic, where the combined use of MTX and cyclosporine showed an advantage over cyclosporine alone (123, 124). Although protocols vary between clinical centers, a combination of calcineurin inhibitors, MTX, and antithymocyte globuline (125) is still the gold standard (126).…”

Bone marrow-derived pancreatic stellate cells in rats

“…4 × 10 7 cells per ml were injected into each irradiated BN/LEW rat via the tail vein. All animals received water containing sulfamethoxazol (0.5 mg/ml), trimethoprim (0.01 mg/ml), tetracycline (0.36 mg/ml) and neomycin (0.58 mg/ml) for 2 weeks [30].…”

Schistosomula, pre-adults and adults ofSchistosoma mansoniingest fluorescence-labelled albuminin vitroandin vivo: implication for a drug-targeting model

“…Albumin carrying the cytostatic drug methotrexate has successfully prevented the onset of rheumatoid arthritis (Wunder et al 2003). It was used for prophylaxis of graft-versus-host disease in animals (Wolff et al 2006) and for cancer treatment in clinical Phase I and II trials (Hartung et al 1999; Vis et al 2002; Bolling et al 2006). Systemic side-effects of methotrexate were greatly reduced when linked to albumin as a carrier.…”