2017
DOI: 10.1016/j.molimm.2017.07.001
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Methotrexate, combined with cyclophosphamide attenuates murine collagen induced arthritis by modulating the expression level of Breg and DCs

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Cited by 11 publications
(3 citation statements)
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“…CP is an effective chemotherapeutic drug that is widely applied as an immunosuppressant in the treatment of various human cancers, including non-Hodgkin’s lymphoma, breast cancer and rheumatoid arthritis. 18 However, previous studies have shown that CP treatment induces serious toxicity, reducing body weight, splenocyte proliferation and leucomonocyte and NK cell activities. 19 In this study, we used CP treatment to establish an immunosuppressed mouse model, and found that CP treatment: reduced spleen and thymus indices; decreased LDH and ACP levels; inhibited Con A- and LPS-induced splenocyte proliferation; decreased NK cell toxicity; decreased IL-2, IL-6, IL-12, TNF-α and IFN-γ levels; increased the IL-10 level; decreased p-IκBα and NF-κB expression; and increased DREAM and IκBα expression.…”
Section: Discussionmentioning
confidence: 99%
“…CP is an effective chemotherapeutic drug that is widely applied as an immunosuppressant in the treatment of various human cancers, including non-Hodgkin’s lymphoma, breast cancer and rheumatoid arthritis. 18 However, previous studies have shown that CP treatment induces serious toxicity, reducing body weight, splenocyte proliferation and leucomonocyte and NK cell activities. 19 In this study, we used CP treatment to establish an immunosuppressed mouse model, and found that CP treatment: reduced spleen and thymus indices; decreased LDH and ACP levels; inhibited Con A- and LPS-induced splenocyte proliferation; decreased NK cell toxicity; decreased IL-2, IL-6, IL-12, TNF-α and IFN-γ levels; increased the IL-10 level; decreased p-IκBα and NF-κB expression; and increased DREAM and IκBα expression.…”
Section: Discussionmentioning
confidence: 99%
“…Normally, the time of the first immunization is referred to as D0 and the time of the second immunization of DBA/1 mice is D21. In most studies, mice are administered injections as preventive treatments on D0-10 and as therapeutic treatments on D21-25 (65)(66)(67)(68)(69). Based on the pathogenesis of the CIA model as described above, the specific development of RA pathology began after the first immunization.…”
Section: Choice Of Intervention Time and Administration Methodsmentioning
confidence: 99%
“…The resulting anti-inflammatory effect is mediated by an accumulation of adenosine that inhibits T cell activation and, in turn, B cells. In connection to autoimmunity, MTX and CYC combined as a treatment of arthritis in mice lead to changes in cellular numbers in lymph nodes and the spleen, including a decrease in Breg and DC numbers [56]. Lastly, azathioprine also constitutes a traditional non-targeted immunosuppressant that is widely used in SLE [44], acting after conversion to 6-mercaptopurine (6-MP), an immunosuppressant prodrug.…”
Section: Anti-inflammatory and Chemotherapy Treatments And Their Impa...mentioning
confidence: 99%