2021
DOI: 10.1016/j.jtv.2020.10.004
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Methotrexate reduces keratinocyte proliferation, migration and induces apoptosis in HaCaT keratinocytes in vitro and reduces wound closure in Skh1 mice in vivo

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Cited by 7 publications
(3 citation statements)
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“…However, it is also used in low doses to effectively treat inflammatory diseases such as rheumatoid arthritis and Crohn’s disease ( 62 , 63 ). The methotrexate doses in animal experiments ranges from 2mg/kg to 200mg/kg ( 64 , 65 ). For the dose in our experiments, due to lack of hamster pharmacokinetics we used the mice pharmacokinetics by Lobo ED’s lab ( 66 ), and to avoid methotrexate-induced toxicity ( 67 ) to consider a lower test dose of 2mg/kg/day for short-term treatment.…”
Section: Methodsmentioning
confidence: 99%
“…However, it is also used in low doses to effectively treat inflammatory diseases such as rheumatoid arthritis and Crohn’s disease ( 62 , 63 ). The methotrexate doses in animal experiments ranges from 2mg/kg to 200mg/kg ( 64 , 65 ). For the dose in our experiments, due to lack of hamster pharmacokinetics we used the mice pharmacokinetics by Lobo ED’s lab ( 66 ), and to avoid methotrexate-induced toxicity ( 67 ) to consider a lower test dose of 2mg/kg/day for short-term treatment.…”
Section: Methodsmentioning
confidence: 99%
“…In a hairless mouse model treated with MTX, there was a delayed proliferation phase that markedly affected the wound-healing process. 6 Moreover, mice treated with high-dose or repeated-dose MTX demonstrated scarred skin, reduced collagen production, and disappearance of skin elements, such as sebaceous glands and hair follicles. Although molecular and preclinical evidence supports MTX's role in impaired wound healing, clinical studies suggest that low-dose MTX is safe and does not affect the incidence of postoperative wound complications.…”
Section: Antineoplastic Agentsmentioning
confidence: 99%
“…Thus, the elicitation of an inflammatory cascade of events is paramount to the body's response to tissue injury. Importantly, immunosuppressive drug regimens used to treat SLE, such as long-term systemic corticosteroids, methotrexate, mycophenolate mofetil, azathioprine, and drugs interfering with the Interleukin-2 mediated immune response (e.g., voclosporin), as well as the presence of SLE per se, counteract and impair the wound healing process [8][9][10][11][12][13]. This potentially complicates the management of burn patients with comorbid SLE and necessitates special consideration on how to approach burn management in this unique population.…”
Section: Introductionmentioning
confidence: 99%