Methoxyflavones from <i>Marcetia taxifolia</i> as HIV-1 Reverse Transcriptase Inhibitors

Ortega, Joseph T.; Serrano, María Luisa Madueño; Suárez, Alírica I.; Baptista, Jani; Pujol, Flor H.; Rangel, Héctor R.

doi:10.1177/1934578x1701201104

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The infection process was performed for one hour at 37 • C in a 5% CO 2 atmosphere, and the cells were washed twice with PBS to retire all the non-internalized viruses. Then, the cells were overlayed with 0.5% carboxymethyl cellulose in a culture medium [31]. The plates were incubated for 72 h, fixed with 4% formaldehyde, and stained with crystal violet to count the number of lytic plaques under each condition.…”

Section: Neutralization Assaymentioning

confidence: 99%

Neutralization of Different Variants of SARS-CoV-2 by a F(ab′)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Rodriguez-Nuñez,

Cepeda,

Bello

et al. 2023

Antibodies

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The Receptor Binding Domain (RBD) of SARS-CoV-2, the virus responsible for the COVID-19 pandemic, is the functional region of the viral Spike protein (S), which is involved in cell attachment to target cells. The virus has accumulated progressively mutations in its genome, particularly in the RBD region, many of them associated with immune evasion of the host neutralizing antibodies. Some of the viral lineages derived from this evolution have been classified as Variant of Interest (VOI) or Concern (VOC). The neutralizing capacity of a F(ab′)2 preparation from sera of horses immunized with viral RBD was evaluated by lytic plaque reduction assay against different SARS-CoV-2 variants. A F(ab′)2 preparation of a hyperimmune serum after nine immunizations with RBD exhibited a high titer of neutralizing antibodies against the ancestral-like strain (1/18,528). A reduction in the titer of the F(ab’)2 preparation was observed against the different variants tested compared to the neutralizing activity against the ancestral-like strain. The highest reduction in the neutralization titer was observed for the Omicron VOC (4.7-fold), followed by the Mu VOI (2.6), Delta VOC (1.8-fold), and Gamma VOC (1.5). Even if a progressive reduction in the neutralizing antibodies titer against the different variants evaluated was observed, the serum still exhibited a neutralizing titer against the Mu VOI and the Omicron VOC (1/7113 and 1/3918, respectively), the evaluated strains most resistant to neutralization. Therefore, the preparation retained neutralizing activity against all the strains tested.

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Section: Neutralization Assaymentioning

confidence: 99%

Neutralization of Different Variants of SARS-CoV-2 by a F(ab′)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Rodriguez-Nuñez,

Cepeda,

Bello

et al. 2023

Antibodies

View full text Add to dashboard Cite

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Flavonoids as Promising Antiviral Agents against SARS-CoV-2 Infection: A Mechanistic Review

et al. 2021

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A newly diagnosed coronavirus in 2019 (COVID-19) has affected all human activities since its discovery. Flavonoids commonly found in the human diet have attracted a lot of attention due to their remarkable biological activities. This paper provides a comprehensive review of the benefits of flavonoids in COVID-19 disease. Previously-reported effects of flavonoids on five RNA viruses with similar clinical manifestations and/or pharmacological treatments, including influenza, human immunodeficiency virus (HIV), severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, were considered. Flavonoids act via direct antiviral properties, where they inhibit different stages of the virus infective cycle and indirect effects when they modulate host responses to viral infection and subsequent complications. Flavonoids have shown antiviral activity via inhibition of viral protease, RNA polymerase, and mRNA, virus replication, and infectivity. The compounds were also effective for the regulation of interferons, pro-inflammatory cytokines, and sub-cellular inflammatory pathways such as nuclear factor-κB and Jun N-terminal kinases. Baicalin, quercetin and its derivatives, hesperidin, and catechins are the most studied flavonoids in this regard. In conclusion, dietary flavonoids are promising treatment options against COVID-19 infection; however, future investigations are recommended to assess the antiviral properties of these compounds on this disease.

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Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

et al. 2021

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Viral infections are among the most complex medical problems and have been a major threat to the economy and global health. Several epidemics and pandemics have occurred due to viruses, which has led to a significant increase in mortality and morbidity rates. Natural products have always been an inspiration and source for new drug development because of their various uses. Among all-natural sources, plant sources are the most dominant for the discovery of new therapeutic agents due to their chemical and structural diversity. Despite the traditional use and potential source for drug development, natural products have gained little attention from large pharmaceutical industries. Several plant extracts and isolated compounds have been extensively studied and explored for antiviral properties against different strains of viruses. In this review, we have compiled antiviral plant extracts and natural products isolated from plants reported since 2015.

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Methoxyflavones from Marcetia taxifolia as HIV-1 Reverse Transcriptase Inhibitors

Cited by 3 publications

References 23 publications

Neutralization of Different Variants of SARS-CoV-2 by a F(ab′)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Neutralization of Different Variants of SARS-CoV-2 by a F(ab′)2 Preparation from Sera of Horses Immunized with the Viral Receptor Binding Domain

Flavonoids as Promising Antiviral Agents against SARS-CoV-2 Infection: A Mechanistic Review

Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

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