Methyl and Ethylmercury elicit oxidative stress and unbalance the antioxidant system in Saccharomyces cerevisiae

Ramos, Angélica; Santos, Matheus Mülling dos; Macedo, Gabriel Teixeira de; Wildner, Guilherme; Prestes, Alessandro de Souza; Masuda, Cláudio A.; Corte, Cristiane Lenz Dalla; Rocha, João Batista Teixeira da

doi:10.1016/j.cbi.2019.108867

Cited by 14 publications

(6 citation statements)

References 56 publications

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“…Oxidative Stress is a type of damage in which the original balance of intracellular pro-oxidant/antioxidant is disturbed and metabolic disorders. Sometimes, it due to the accumulation of reactive oxygen species (ROS) which mainly comes from the peroxisomes, endoplasmic reticulum (ER) or mitochondria [50,51], or it causes by electrophilic compounds, such as cobalt, mercury, furfural and ferulic acid [52][53][54]. Innately, overcoming oxidative stress depend on different TFs-related signaling which reconstruct metabolic networks in yeast [55].…”

Section: Oxidative Stress Response Modulementioning

confidence: 99%

Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

Dong¹,

Qin²,

Li³

et al. 2020

Preprint

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Section: Oxidative Stress Response Modulementioning

confidence: 99%

Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

Dong¹,

Qin²,

Li³

et al. 2020

Preprint

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“…The yeast Saccharomyces cerevisiae is treated as a model organism due to its homology to eukaryotic organisms. For this reason, their enzymatic response to oxidative stress has been widely analyzed in numerous studies [28][29][30]. However, there are still no reports on the possible impact of mycotoxins such as AFB 2 +G 1 , OTA, and ZEA on the activation of the antioxidant system of Saccharomyces cerevisiae and the production of heat shock proteins in these microorganisms.…”

Section: Introductionmentioning

confidence: 99%

The Reaction of the Yeast Saccharomyces cerevisiae to Contamination of the Medium with Aflatoxins B2 and G1, Ochratoxin A and Zearalenone in Aerobic Cultures

Kłosowski,

Koim-Puchowska,

Dróżdż-Afelt

et al. 2023

IJMS

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The mechanisms by which yeast cells respond to environmental stress include the production of heat shock proteins (HSPs) and the reduction of oxidative stress. The response of yeast exposed to aflatoxins B2+G1 (AFB2+G1), ochratoxin A (OTA), and zearalenone (ZEA) in aerobic conditions was studied. After 72 h of yeast cultivation in media contaminated with mycotoxins, the growth of yeast biomass, the level of malondialdehyde, and the activity of superoxide dismutase, glutathione S-transferase and glutathione peroxidase were examined; the expression profile of the following heat shock proteins was also determined: HSP31, HSP40, HSP60, HSP70, and HSP104. It was demonstrated that at the tested concentrations, both AFB2+G1 and ZEA inhibited yeast biomass growth. OTA at a concentration of 8.4 [µg/L] raised the MDA level. Intensified lipoperoxidation and increased activity of SOD and GPx were observed, regardless of the level of contamination with ZEA (300 µg/L or 900 µg/L). Increased contamination with AFB2+G1 and OTA caused an increase in the production of most HSPs tested (HSP31, HSP40, HSP70, HSP104). ZEA contamination in the used concentration ranges reduced the production of HSP31. The response of yeast cells to the presence of mycotoxin as a stressor resulted in the expression of certain HSPs, but the response was not systematic, which was manifested in different profiles of protein expression depending on the mycotoxin used. The tested mycotoxins influenced the induction of oxidative stress in yeast cells to varying degrees, which resulted in the activation of mainly SOD without GST mobilization or with a small involvement of GPx.

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“…Thimerosal contains a mercury (Hg) atom bound to a thiosalicylate moiety and an ethyl group [ 19 ]. Thimerosal became part of the composition of cosmetics, vaccines, and pharmaceutical products, such as Merthiolate ® , due to its fungicidal and antibacterial properties [ 20 , 21 ]; however, some researchers have suggested that thimerosal is more toxic to human cells than to bacteria [ 22 ]. Inside the organism, the mercury–sulfur bond of the thimerosal molecule breaks, releasing thiosalicylate and ethylmercury (EtHg) [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Since several studies have explored the toxicity of aluminum hydroxide [ 30 , 31 , 32 , 33 ] and isolated thimerosal [ 21 , 34 , 35 , 36 , 37 ] and these compounds are found together in the formulation of some multidose vaccines applied in developing countries, such as Brazil [ 4 ], it is relevant to study their toxicological effects in combination. Thus, this study aimed to analyze the toxic effects of metal-containing molecules used as adjuvants and preservatives (aluminum hydroxide and thimerosal, respectively) in the formulation of vaccines using D. rerio as an experimental model.…”

Section: Introductionmentioning

confidence: 99%

Toxicological Effects of Thimerosal and Aluminum in the Liver, Kidney, and Brain of Zebrafish (Danio rerio)

Galiciolli,

Silva,

Prodocimo

et al. 2023

Metabolites

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Vaccination programs in the first years of a child’s life are effective and extremely important strategies for the successful eradication of diseases. However, as no intervention is without risks, the metal-based components of some vaccines, such as thimerosal (TMS), a preservative composed of ethylmercury, and aluminum (Al), have begun to generate distrust on the part of the population. Therefore, this study evaluated the effects of exposure to thimerosal and aluminum hydroxide (alone or in mixture) on Danio rerio (zebrafish) specimens. The fish were exposed to thimerosal and/or aluminum hydroxide intraperitoneally. The liver, kidney, and brain were removed for a biochemical biomarker analysis, histopathological analysis, and metal quantification. As a result, we observed changes in the activity of the analyzed enzymes (SOD, GST, GPx) in the kidney and brain of the zebrafish, a reduction in GSH levels in all analyzed tissues, and a reduction in MT levels in the kidney and liver as well as in the brain. Changes in AChE enzyme activity were observed. The biochemical results corroborate the changes observed in the lesion index and histomorphology sections. We emphasize the importance of joint research on these compounds to increase the population’s safety against their possible toxic effects.

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Methyl and Ethylmercury elicit oxidative stress and unbalance the antioxidant system in Saccharomyces cerevisiae

Cited by 14 publications

References 56 publications

Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

Transcription Factor Engineering Harnesses Metabolic Networks to meeting industrial requirements in Yeast

The Reaction of the Yeast Saccharomyces cerevisiae to Contamination of the Medium with Aflatoxins B2 and G1, Ochratoxin A and Zearalenone in Aerobic Cultures

Toxicological Effects of Thimerosal and Aluminum in the Liver, Kidney, and Brain of Zebrafish (Danio rerio)

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