Methyl farnesoate stimulates gonad development in Macrobrachium malcolmsonii (H. Milne Edwards) (Decapoda, Palaemonidae)

Suraj, Suraj; Nagaraju, Ganji Purnachandra; Reddy, P. Sreenivasula

doi:10.1163/156854003773123401

Cited by 38 publications

(15 citation statements)

References 31 publications

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“…This observation correlates with the high TIs of red-phase animals, consistent with the idea that MF stimulates testicular growth either directly or indirectly in crustaceans (Kalavathy et al, 1999;Nagaraju et al, 2003;Nagaraju et al, 2006;Reddy et al, 2004). These observations are similar to previous studies in the spider crab (Libinia emarginata), which demonstrated a correlation between the reproductive status of males and MF synthesis by the mandibular organ (MO) and hemolymph levels of MF (Sagi et al, 1993;Sagi et al, 1994).…”

Section: Discussionsupporting

confidence: 90%

“…Likewise, MF synthesis rates by spider crab MO incubated in vitro were correlated with reproductive behavior (Sagi et al, 1993;Sagi et al, 1994). In addition, MF treatment has been shown to increase testicular growth in a number of crustacean species, including the freshwater field crab O. senex senex (Kalavathy et al, 1999;Reddy et al, 2004), and the freshwater prawn Macrobrachium malcholamsonii (Nagaraju et al, 2003). Our observations confirm these previous studies and demonstrate that MF treatment can stimulate testicular growth in green-phase animals.…”

Section: T-test) (B)supporting

confidence: 87%

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Methyl farnesoate couples environmental changes to testicular development in the crabCarcinus maenas

Nagaraju

Borst

2008

Journal of Experimental Biology

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SUMMARYCarcinus maenas males have two major color phases. Green-phase males molt frequently and tend to live in brackish estuaries during the summer. After becoming red-phase males, they molt infrequently, have higher mating success, and live in cooler, deeper water. We found profound differences between these two phases in the way salinity and temperature affect hemolymph levels of methyl farnesoate (MF), a hormone that affects crustacean reproduction. Few green-phase males (<10%) had detectable MF in 33 ppt seawater (SW) at 11 or 18°C. By contrast, about 30% of the red-phase males had detectable MF at either temperature. After transfer to 5 ppt SW, none of the green-phase males had detectable MF at 11°C whereas 100% of green-phase males did at 18°C. By contrast, 100% of the red-phase males had detectable MF in 5 ppt SW at either temperature. At 11°C, green-phase males had detectable MF after eyestalk ablation (ESA), showing that they can produce MF. There was no additional increase in MF levels when ESA animals of either color phase were transferred to 5 ppt SW, suggesting that the eyestalk is the primary regulator of the MF response to low salinity. MF levels of green-phase males were increased by injecting MF, by ESA, or by exposure to 5 ppt SW at 18°C. The testicular index of these treated animals nearly doubled after two weeks. Our results strongly suggest that environmental conditions such as temperature and salinity, affect testicular development in this crab by changing its MF levels.Key words: methyl farnesoate, testis, crustacean, salinity, reproduction, temperature. THE JOURNAL OF EXPERIMENTAL BIOLOGY 2774In the present study, we show that red-phase crabs are more likely to have detectable MF in their hemolymph than green-phase crabs. In addition, the testicular index (TI) of red-phase animals is higher than that in green-phase animals. Furthermore, our results indicate that environmental factors differentially affect MF levels in the two color phases. These results are consistent with the hypothesis that MF stimulates testicular development. To test this hypothesis, we show that environmental factors that increase MF levels also stimulate testicular development. The MF levels in these two color phases appear to be responsible, in part, for the different reproductive activities of these males. MATERIALS AND METHODS AnimalsCrabs [Carcinus maenas (Linnaeus 1758)] were generously provided by Dan Landers (Millstone Environmental Lab; Waterford, CT, USA). Most of the males used in this study were 50±5 g (carapace width, CW 5.2±0.6 cm) and were collected during the spring and early summer. Green-phase and red-phase animals were identified by the color of their ventral surface (Reid et al., 1997). None of the experimental animals was missing appendages. At the end of some experiments, the testes were removed and weighed, and the TI (% total body mass) was calculated. Crabs were kept in large tanks containing artificial SW (Instant Ocean, Spectrum Brands; Atlanta, GA, USA) at normal salinity (33 ppt) at 11 o...

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Section: Discussionsupporting

confidence: 90%

Section: T-test) (B)supporting

confidence: 87%

Methyl farnesoate couples environmental changes to testicular development in the crabCarcinus maenas

Nagaraju

Borst

2008

Journal of Experimental Biology

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“…No mortality was observed in MF injected group and observed all of them are in late vitellogenic stage (III) with significant increase (p<0.0001) in ovarian index (508.84%), oocyte diameter (1004.16%) and ovarian vitellogenin levels (137.57%) ( Table 1). MF is known to be involved in the regulation of reproduction in various crustaceans [18,25,28,[33][34][35][36][37]. Similar to ovarian index, oocyte diameter and ovarian vitellogenin levels, observed significant increase (p<0.0001) in the ovarian total lipid and protein in both ESX and MF injected prawns ( In MF injected animals, the lipid and protein levels were significantly increased when compared with control and eyestalk ablated animals.…”

Section: Resultsmentioning

confidence: 84%

“…In specific MF is a positive regulator for reproduction and is negatively controlled by mandibular organ inhibiting hormone (MOIH) [2,[16][17][18][19] which suppressed the synthesis and release of MF from MO. An evidence of MF on regulation of reproduction has been reported in the crab Oziothelphusa senex senex [17,19], the crayfish Procambarus clarkii [20], the prawn Machrobrachium malcamsonii [18] and the shrimp Penaeus vannameii [21]. The action of MF on reproduction in crustaceans is not yet established.…”

Section: Introductionmentioning

confidence: 99%

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Methyl Farnesoate Induced Ovarian Maturation in Freshwater Prawn, Macrobrachium rosenbergii

Hemalatha¹,

Parameswari²,

Kishori³

et al. 2017

J Oceanogr Mar Res

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The present study focused to elucidate the effect of methyl farnesoate (MF) on ovarian development and maturation in the freshwater giant prawn, Macrobrachium rosenbergii. Control, eyestalk ablated (ESX) and MF injected prawns were maintained during the experimentation and observed significant (p<0.001) increase in the mean ovarian index, oocyte diameter and ovarian vitellogenin levels in both eyestalk ablated and methyl farnesoate administered prawns when compared with controls. All the MF injected animals are in vitellogenic stage III, whereas ESX animals were showed all the reproductive stages at 30th day of experiment. The levels of protein and lipid in the ovary were also measured. The total protein and lipid content levels were also significantly increased in eyestalk ablated (145.07% and 72.05% respectively) and MF injected animals (315.21% and 118.39% respectively) than the controls. The outcome of the present study clearly suggests that MF stimulates the reproduction in female prawns than the conventional ESX method.

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What Do We (Need to) Know About the Melatonin in Crustaceans?

2013

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Melatonin (N-acetyl-5-methoxy-tryptamine) was first discovered from the bovine pineal gland extract in 1958. Since then, its synthesis, metabolism, physiological, and patho-physiological functions are well studied in vertebrates; there is an increasing recognition of melatonin in invertebrates and especially in crustaceans. The presence of melatonin in crustaceans is now well documented and some functional aspects in the framework of crustacean biology have been demonstrated. This review aims at giving a comprehensive overview of the various physiological events regulated by this pleiotropic hormone. Topics include: glucose homeostasis, regulation of reproduction, molting, limb regeneration, and antioxidant properties. Finally, perspectives on current and possible research are offered.

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Methyl farnesoate stimulates gonad development in Macrobrachium malcolmsonii (H. Milne Edwards) (Decapoda, Palaemonidae)

Cited by 38 publications

References 31 publications

Methyl farnesoate couples environmental changes to testicular development in the crabCarcinus maenas

Methyl farnesoate couples environmental changes to testicular development in the crabCarcinus maenas

Methyl Farnesoate Induced Ovarian Maturation in Freshwater Prawn, Macrobrachium rosenbergii

What Do We (Need to) Know About the Melatonin in Crustaceans?

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