Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures

Abstract

Abstract:To investigate the enzyme variations responsible for the synthesis of phenolics, 40 day-old adventitious roots of Panax ginseng were treated with 200 µM methyl jasmonate (MJ) or salicylic acid (SA) in a 5 L bioreactor suspension culture (working volume 4 L). Both treatments caused an increase in the carbonyl and hydrogen peroxide (H 2 O 2 ) contents, although the levels were lower in SA treated roots. Total phenolic, flavonoid, ascorbic acid, non-protein thiol (NPSH) and cysteine contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reducing activity were increased by MJ and SA. Fresh weight (FW) and dry weight (DW) decreased significantly after 9 days of exposure to SA and MJ. The highest total phenolics (62%), DPPH activity (40%), flavonoids (88%), ascorbic acid (55%), NPSH (33%), and cysteine (62%) contents compared to control were obtained after 9 days in SA treated roots. The activities of glucose 6-phosphate dehydrogenase, phenylalanine ammonia lyase, substrate specific peroxidases (caffeic acid peroxidase, quercetin peroxidase and ferulic acid peroxidase) were higher in MJ treated roots than the SA treated ones. Increased shikimate dehydrogenase, chlorogenic acid peroxidase and β-glucosidase activities and proline content were observed in SA treated roots than in MJ ones. Cinnamyl alcohol Molecules 2007, 12 608 dehydrogenase activity remained unaffected by both MJ and SA. These results strongly indicate that MJ and SA induce the accumulation of phenolic compounds in ginseng root by altering the phenolic synthesis enzymes.

Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant

“…3a, b and c). The fact that Me-J influences the secondary metabolism of plants is already established and reported (Ali et al 2007). Generally, phenolics and flavonoids are anti-oxidative in nature and are produced in the phenyl-propanoid pathway (Heller and Forkmann 1988;Lister et al 1996).…”

Functional Promiscuity of Two Divergent Paralogs of Type III Plant Polyketide Synthases

“…Moreover, the exogenous treatment of plants with SA broadly displayed a transcript profile of paralogous ReCHS genes that was found to be in consonance with the increasing content of both flavonoids and anthraquinones. Additionally, several studies have shown that MeJ and SA are prospective candidates for the elicitation process leading to the transcriptional alteration of various genes that ultimately affect secondary metabolite biosynthesis (Ali et al, 2007).…”

“…9J; Supplemental Table S2). SA treatments also have resulted in significant increases in the flavonoid content in root suspension cultures of Panax ginseng (Ali et al, 2007).…”

Niveau De Connaissance De La Ménopause Et Habitudes Alimentaire Et Médicinale Des Femmes En Côte d’Ivoire

“…Ces composés, notamment les isoflavones sont également capables d'améliorer les troubles vasomoteurs en diminuant l'intensité et la fréquence des bouffées de chaleur (Amiot et al, 2012), de réduire la résorption osseuse et de stimuler la formation osseuse (Lecerf, 2007). Les polyphénols, de par leurs activités anti-inflammatoires et analgésiques (Babar Ali et al, 2007), sont capables de soulager les femmes des douleurs musculaires et articulaires qu'elles pourraient ressentir.…”