Methyl jasmonate as a control factor of the synthase squalene gene promoter and ginsenoside production in American ginseng hairy root cultured in shake flasks and a nutrient sprinkle bioreactor

Kochan, Ewa; Balcerczak, Ewa; Lipert, Anna; Szymańska, Grażyna; Szymczyk, Piotr

doi:10.1016/j.indcrop.2018.02.036

Cited by 37 publications

(40 citation statements)

References 65 publications

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“…The research on P. notoginseng suspension cell cultures showed a stronger, approximately 9-fold increase of Rb1 concentration after MJ treatment (30). Similar observations were noted in hairy and adventitious root cultures of P. quinquefolium and P. ginseng growing in the shake lasks (12,13,14). In our study Rc and Rb 1 metabolites were not detected at 500 μmol L -1 concentration of MJ.…”

Section: Resultssupporting

confidence: 87%

“…Stimulating effect of methyl jasmonate on ginseng saponin production may be explained on the genetic level. MJ treatment greatly enhances the expression of the key genes involved in the ginsenoside biosynthesis pathway and increases the quantity of major intermediates such as: squalene, 2,3-oxidosqualene, and dammarenediol II that are precursors for biosynthesis of ginsenosides (14,17).…”

Section: Resultsmentioning

confidence: 99%

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Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

Kochan

Caban

Szymańska

et al. 2018

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<p>Panax quinquefolium L., belonging to the Araliaceae family, along with P. ginseng is one of the well-known species of ginseng. Multidirectional pharmacological action of this plant is attributed to triterpene saponins called ginsenosides. Pharmacopoeial raw material are roots obtained from the field crops which are time-consuming and require expensive agrotechnical procedures. Therefore, the new sources of ginseng biomass are sought such as in vitro suspension cultures. P. quinquefolium L. cell cultures, treated with the elicitation of methyl jasmonate (MJ) in concentration 50 and 250 μmol L-1, synthesize more ginsenosides than control cultures. The highest increase (2.2-fold) of all examined compounds was noted using 250 μmol L-1 MJ. In this condition, the predominantly quantitative metabolite was Rb1 ginsenoside belonging to protopanaxadiol derivatives.</p>

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

Kochan

Caban

Szymańska

et al. 2018

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“…JA, MJ, SA, and ASA have been recognized as another class of signal transducer in elicitation of plant defense responses [30,42,[65][66][67][68][69][70][71][72][73][74]. Application of MJ, JA, SA, ASA, and other signal molecules may or may not inhibit growth and thus effect biomass accumulation during the enhancement of SMs production or vice-versa [8,42].…”

Section: Effect Of Signal Molecules On Sm Productionmentioning

confidence: 99%

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

238

116

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Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

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“…Our own previous studies demonstrate that hairy root cultures of P. quinquefolium can produce comparable amounts of ginseng saponins to naturally grown roots, but in a significantly shorter time, with only 28 days being needed for cultivation. This increased production was made possible using an elicitation protocol [ 6 , 7 ]. Elicitation is regarded as one of most effective biotechnological tools for intensifying the biosynthesis of useable plant metabolites, with fungal cell extracts, polysaccharides from fungal and plant cells, jasmonates and other hormones, UV radiation, hyperosmotic stress, temperature, and heavy metal salts being commonly used to improve the yield secondary metabolites in a range of hairy root cultures [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…While our previous studies have used typical elicitors such as yeast extract or methyl jasmonate [ 6 , 7 ], the aim of the present study was to determine whether trans -anethole can be used as potential new elicitor to enhance triterpene saponin production in P. quinquefolium hairy roots cultivated in shake flasks. Such use of essential oil components for elicitation is currently not documented.…”

Section: Introductionmentioning

confidence: 99%

The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium

et al. 2018

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In vitro cultivation is an effective way to increase pharmaceutical production. To increase ginsenoside production in hairy root cultures of American ginseng, the present study uses trans-anethole as an elicitor. The content of nine triterpene saponins was determined: Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, Re and Rf. Trans-anethole was found to stimulate saponin synthesis regardless of exposure time (24 and 72 h). Twenty-four hour exposure to 1 μmol trans-anethole in the culture medium resulted in the highest increase of total saponin content (twice that of untreated roots), and optimum accumulation of Rb-group saponins, with ginsenoside Rc dominating (8.45 mg g−1 d.w.). In contrast, the highest mean content of protopanaxatriol derivatives was obtained for 10 μmol trans-anethole. The Re metabolite predominated, reaching a concentration of 5.72 mg g−1 d.w.: a 3.9-fold increase over untreated roots. Elicitation with use of trans-anethole can therefore be an effective method of increasing ginsenoside production in shake flasks.

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Methyl jasmonate as a control factor of the synthase squalene gene promoter and ginsenoside production in American ginseng hairy root cultured in shake flasks and a nutrient sprinkle bioreactor

Cited by 37 publications

References 65 publications

Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

The Increase of Triterpene Saponin Production Induced by Trans-Anethole in Hairy Root Cultures of Panax quinquefolium

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