Methyl jasmonate leads to necrosis and apoptosis in hepatocellular carcinoma cells via inhibition of glycolysis and represses tumor growth in mice

Li, Jingjing; Chen, Kan; Wang, Fan; Dai, Weiqi; Li, Sainan; Jiao, Feng; Wu, Liwei; Liu, Tong; Xu, Shizan; Xia, Yujing; Lü, Jie; Zhou, Yingqun; Xu, Ling; Guo, Chuanyong

doi:10.18632/oncotarget.17469

Cited by 32 publications

(38 citation statements)

References 46 publications

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“…This conclusion was consistent with the results of Sumi et al' [44] and Ding et al' [32], showing that glycolysis and apoptosis of cells could be improved simultaneously. But it was not in agreement with the ndings of Li et al' [43] and Comin-Anduix et al' [40], who showing that glycolysis of cells was inhibited and apoptosis of cells was enhanced, possible owing to differences in the type of cell. Glycolysis is the main source of energy in cancer cells, which use this metabolic pathway for ATP generation.…”

Section: Discussioncontrasting

confidence: 68%

Salmonella enterica serovar Typhimurium sseK3 induces apoptosis and enhances glycolysis in macrophages

Chen

Zhang

et al. 2020

Preprint

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Background: Salmonella enterica serovar Typhimurium ( S. Typhimurium) is an important infectious disease pathogen that can survive and replicate in macrophages. Glycolysis is essential for immune responses against S. Typhimurium infection in macrophages, and is also associated with apoptosis. S. Typhimurium secreted effector K3 (SseK3) was recently identified as a novel translated and secreted protein. However, there is no study about the role of sseK3 in the relationship between apoptosis and glycolysis in cells infected with S. Typhimurium. It is unclear whether this protein exerts a significant role in the progress of apoptosis and glycolysis in S. Typhimurium-infected macrophages. Results: Macrophages were infected with S. Typhimurium SL1344 wild-type (WT), Δ sseK3 mutant or sseK3 -complemented strain, and the effects of sseK3 on apoptosis and glycolysis were determined. The adherence and invasion in the Δ sseK3 mutant group were similar to that in the WT and sseK3 -complemented groups, indicating that SseK3 was not essential for the adherence and invasion of S. Typhimurium in macrophages. However, the percentage of apoptosis in the Δ sseK3 mutant group was much lower than that in the WT and sseK3 -complemented groups. Caspase-3, caspase-8, and caspase-9 enzyme activity in the Δ sseK3 mutant group were significantly lower than in the WT group and sseK3 -complemented groups, indicating that sseK3 could improve the caspase-3, caspase-8, and caspase-9 enzyme activity. We also found that there were no significant differences in pyruvic acid levels between the three groups, but the lactic acid level in the Δ sseK3 mutant group was much lower than that in the WT and sseK3 -complemented groups. The ATP levels in the Δ sseK3 mutant group were remarkably higher than those in the WT and sseK3 -complemented groups. These indicated that the sseK3 enhanced the level of glycolysis in macrophages infected by S. Typhimurium. Conclusions: S. Typhimurium sseK3 is likely involved in promoting macrophage apoptosis and modulating glycolysis in macrophages. Our results could improve our understanding of the relationship between apoptosis and glycolysis in macrophages induced by S. Typhimurium sseK3 .

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Section: Discussioncontrasting

confidence: 68%

Salmonella enterica serovar Typhimurium sseK3 induces apoptosis and enhances glycolysis in macrophages

Chen

Zhang

et al. 2020

Preprint

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“…MeJA's cytotoxic activity against cancer cells is reported to occur as consequence of one or more of the following events: (I) increased ROS production in mitochondria of cancer cells, which are more sensitive than normal cells to higher ROS concentrations [ 2 ], and (II) modification of the mitochondrial respiration of cancer cells which provokes ATP depletion [ 1 ]; and (III) impaired glucose metabolism through glycolysis, due to hexokinase inhibition [ 50 ]. The results of this study show that hepatic cells of both healthy and arthritic rats respond to MeJA, at least partly, in the same way as cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Anti‐Inflammatory and Antioxidant Actions of Methyl Jasmonate Are Associated with Metabolic Modifications in the Liver of Arthritic Rats

Sá‐Nakanishi

Soni-Neto

Moreira

et al. 2018

Oxidative Medicine and Cellular Longevity

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Methyl jasmonate (MeJA) is a fatty acid-derived cyclopentanone which shares structural similarities with prostaglandins and has been under study as a promising anti-inflammatory agent. This study investigated the actions of MeJA on systemic inflammation and oxidative status in rats with adjuvant-induced arthritis, a model for rheumatoid arthritis. MeJA (75 to 300 mg·kg−1) was administrated orally during 18 days after arthritis induction with Freund's adjuvant. Articular and systemic inflammation was greatly increased in arthritic rats, likewise the oxidative stress in plasma and liver. The hepatic glucokinase activity and glycolysis were increased in arthritic rats. MeJA decreased most inflammatory parameters and abolished the increased protein carbonylation in plasma and liver, diminished the increased hepatic ROS content, and restored the hepatic GSH/GSSG ratio in arthritic rats. However, the MeJA treatment decreased the hepatic glucokinase activity and glycolysis and stimulated mitochondrial ROS production in healthy and arthritic rats. Oxygen uptake was increased by MeJA only in livers from treated arthritic rats. This action may bear relation to the increased activity of mitochondrial NADP+-dependent enzymes to provide reducing equivalents for the glutathione cycle. These beneficial effects, however, are associated with a decreased glucose flux through the glycolysis in the liver of arthritic and healthy rats.

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“…The conclusion was consistent with Sumi's [42] and Ding's [33] results that both glycolysis and apoptosis of cells could be improved. It was contradict with Li's [41] and Comin-Anduix's [38] results that glycolysis of cells was inhibited and apoptosis of cells was enhanced. There may be the mainly reason is the type of cell.…”

Section: Discussionmentioning

confidence: 84%

“…Recently, There has shown that the glycolysis could be inhibited and the cell apoptosis was further blocked [40]. Li found that methyl jasmonate could inhibit the glycolysis and induce apoptosis of tumor cells [41]. Sumi found that propofol could improve glycolysis of cells and lead to the apoptosis of cells [42].…”

Section: Discussionmentioning

confidence: 99%

The Salmonella enterica serovar Typhimurium sseK3 induce cell apoptosis and enhance glycolysis in macrophages

Chen

Zhang³

et al. 2020

Preprint

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Background: Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important infectious disease pathogen. Recently, there are increasing researches about the relationship between apoptosis and glycolysis of cells. Previous studies have identified that S. Typhimurium secreted effector K3 (SseK3) is a novel translated and secreted protein. However, there is no study about the role of sseK3 in the relationship between apoptosis and glycolysis of cells infected with S. Typhimurium. It is unclear whether this protein exerts a significant role in the progress of apoptosis and glycolysis in macrophages. Results: The S. Typhimurium SL1344 wild-type (WT) group, ΔsseK3 mutant group and sseK3-complemented group were used to infect macrophages and the effects of sseK3 on apoptosis and glycolysis of macrophages were investigated. The adherence and invasion of ΔsseK3 mutant group for macrophages were similar to WT group and sseK3-complemented group, indicating that SseK3 did not play an important role in the adherence and invasion of S. Typhimurium for macrophages. However, the apoptosis percentage of the ΔsseK3 mutant group was much lower than WT group and sseK3-complemented group using flow cytometry. The caspase-3, caspase-8 and caspase-9 enzyme activity of the ΔsseK3 mutant group were decreased significantly compared with WT group and sseK3-complemented group, which suggested that sseK3 could improve the activities of caspase-3, caspase-8 and caspase-9 enzyme. We also found that the pyruvic acid level did not significantly change among ΔsseK3 mutant group, WT group and sseK3-complemented group, but the lactic acid level of ΔsseK3 mutant group was much lower than WT group and sseK3-complemented group. The ATP level of ΔsseK3 mutant group was remarkably higher than WT group and sseK3-complemented group. These indicated that the sseK3 enhanced the level of glycolysis of macrophages infected by S. Typhimurium. Conclusions: Our data showed that the sseK3 of S. Typhimurium involved in promoting macrophages apoptosis and influencing glycolysis levels of macrophages. These results may give a better clue about the relationship between apoptosis and glycolysis in macrophages induced by S. Typhimurium sseK3.

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Methyl jasmonate leads to necrosis and apoptosis in hepatocellular carcinoma cells via inhibition of glycolysis and represses tumor growth in mice

Cited by 32 publications

References 46 publications

Salmonella enterica serovar Typhimurium sseK3 induces apoptosis and enhances glycolysis in macrophages

Salmonella enterica serovar Typhimurium sseK3 induces apoptosis and enhances glycolysis in macrophages

Anti‐Inflammatory and Antioxidant Actions of Methyl Jasmonate Are Associated with Metabolic Modifications in the Liver of Arthritic Rats

The Salmonella enterica serovar Typhimurium sseK3 induce cell apoptosis and enhance glycolysis in macrophages

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