Methyl Jasmonate Promotes Phospholipid Remodeling and Jasmonic Acid Signaling To Alleviate Chilling Injury in Peach Fruit

Abstract

Chilling injury (CI) is a physiological disorder induced by cold, which heavily limit crop production and postharvest preservation worldwide. Methyl jasmonate (MeJA) can alleviate CI in various fruit species, including peach; however, the underlying molecular mechanism is poorly understood. Here, changes in contents of phenolics, lipids, and jasmonic acid (JA) and gene expressions are compared between MeJA and control fruit. Exogenous MeJA inhibited expressions of PpPAL1, PpPPO1, and PpPOD1/2 but did not affect the phenolic content. Furthermore, MeJA fruit showed lower relative electrolyte leakage, indicating less membrane damage. Meanwhile, the enrichment of linoleic acid in the potential lipid biomarkers, especially phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol, coincided with lower expressions of PpFAD8.1 but higher PpLOX3.1 and JA content. In the JA signaling pathway, MeJA significantly upregulated expressions of PpMYC2.2 and PpCBF3 but downregulated PpMYC2.1. In conclusion, adjustments of fatty acids in phospholipids contribute to MeJA-induced alleviation of CI in peach fruit via induction of the JA-mediated C-repeat-binding factor pathway.

Revealing Further Insights on Chilling Injury of Postharvest Bananas by Untargeted Lipidomics

“…Changes in lipid compositions and structures have been increasingly reported in postharvest of fruits in response to chilling stress [ 13 , 14 , 29 ]. However, none of these studies displays distinctive differences in postharvest bananas during chilling injury, including lipids degradation, peroxidation, and critical enzymes involved in lipid metabolism, indicating a need for a more comprehensive analysis of lipid changes in postharvest bananas exposed to chilling stress.…”

“…Similar results were obtained in anthurium cut flowers and bell pepper [ 13 , 34 ]. Recent studies indicated that methyl jasmonate treatment increased the levels of phospholipid biomarkers of peach fruit (especially PC and PE), promoted phospholipid remodeling, and consequently alleviated chilling injury [ 14 ].…”

“…Recently, it has been shown that membrane lipid compositions alter significantly during chilling injury of bell peppers at 4 °C while PC, PE, PA, and MGDG are determined to be the crucial biomarkers in the chilling injury process [ 13 ]. PC, PE and PG function as the main components of the membrane lipids in relation to the chilling injury of peach fruit during cold storage [ 14 , 15 ]. PA was reported to be the degradation product of the structural phospholipids while the accumulation of PA could further promote the production of free radicals and hydroperoxides and then lead to severe membrane damage [ 16 , 17 ].…”

“…Combined lipidomic and transcriptomic analyses have been reported to explore the wax formation with respect to the involvement of membrane lipids in citrus fruit [ 25 ]. Chen et al [ 14 ] found that methyl jasmonate promoted phospholipid remodeling to alleviate chilling injury in peach fruit by using untargeted lipidomic analysis. Thus, through application of lipidomic analysis, we can obtain information on lipid families and lipid molecules in various biological processes and can help to elucidate the relevant biological processes.…”

Regulatory influences of methyl jasmonate and calcium chloride on chilling injury of banana fruit during cold storage and ripening

“…Chilling injury is a physiological disorder, which usually enhances postharvest losses worldwide (Chen et al, 2019). Cell membrane dysfunction occurs during storage at low temperatures and finally leads to CI development in various fruits and vegetables (Rui et al, 2010), whereby cell membrane is damaged by increasing reactive oxygen species (ROS).…”