Methyl- rich diet ameliorates lupus-like disease in MRL/lpr mice

Nikolova-Ganeva, Kalina; Bradyanova, Silvya; Manoylov, Ilian; Boneva, Gabriela; Tchorbanov, Andrey

doi:10.1016/j.imbio.2022.152282

Cited by 2 publications

(3 citation statements)

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“…Тwo types of rodent diet were purchased from Envigo (Indianapolis, IN, USA): standard rodent diet and high‐dose methyl diet containing the following ingredients for 8 g of daily portion per mouse (Table 1) [22]. Three groups of female Balb/c mice ( n = 10 mice per group, 8 weeks old) received a single ip injection of 500 μL of 2,6,10,14‐tetramethylpentadecane mineral oil (Pristane) (Sigma‐Aldrich) and were further treated as follows: the first group of experimental animals was fed a standard diet (ND group); the second group of animals was fed a high‐dose methyl diet (supplemented group, SD) during the whole course of the experiment, and the third group was fed a standard diet from the eighth week of age and 16 weeks later, after appearance of proteinuria, the diet was switched to the high‐dose methyl diet (therapeutic group, ND‐SD) (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…Тwo types of rodent diet were purchased from Envigo (Indianapolis, IN, USA): standard rodent diet and highdose methyl diet containing the following ingredients for 8 g of daily portion per mouse (Table 1) [22]. Three groups of female Balb/c mice (n = 10 mice per group, 8 weeks old) received a single ip injection of 500 μL of 2,6,10,14-tetramethylpentadecane mineral oil (Pristane) (Sigma-Aldrich) and were further treated as follows: the T A B L E 1 Amounts of the components (mg/8 g diet) in the standard diet and in the high-dose methyl diet.…”

Section: Treatment Schedulementioning

confidence: 99%

“…We have recently reported that the administration of high amounts of particular nutrients in the diet of autoimmune‐prone MRL/ lpr mice resulted in decreased levels of proteinuria and of anti‐dsDNA antibodies, prevention of skin lesions and reduced kidney pathology [22]. The diet consisted of extra amounts of methyl‐containing components—choline, betaine, methionine, folic acid, zinc, and vitamin D, all of which are important participants in the DNA methylation processes.…”

Section: Introductionmentioning

confidence: 99%

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Methyl‐supplemented nutrition delays the development of autoimmune disease in pristane‐induced murine lupus

Bradyanova,

Manoylov,

Boneva

et al. 2024

Immunology

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The aetiology and progression of systemic lupus erythematosus (SLE) resulted from a complex sequence of events generated both from genetic and epigenetic processes. In the current research, the effect of methyl‐supplemented nutrition on the development of SLE was studied in the pristane‐induced mouse model of the disease. The results clearly demonstrated decreased anti‐dsDNA antibody and proteinuria levels, modulation of cytokines and protected renal structures in the group of treated mice. An additional increase in the DNA methylation of mouse B lymphocytes was also observed. The beneficial effect of the diet is due to the methyl‐containing micronutrients with possible anti‐inflammatory and immunomodulating effects on cell proliferation and gene expression. Since these components are responsible for maintaining the physiological methylation level of DNA, the results point to the central role of methylation processes in environmentally triggered lupus. As nutrition represents one of the major epigenetic factors, these micronutrients may be considered novel agents with significant therapeutic outcomes.

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Section: Methodsmentioning

confidence: 99%

Section: Treatment Schedulementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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