Methylation-associated silencing of miR-495 inhibit the migration and invasion of human gastric cancer cells by directly targeting PRL-3

Li, Zhengrong; Zhang, Guoyang; Li, Daojiang; Jie, Zhigang; Chen, Heping; Xiong, Jianbo; Liu, Yi; Cao, Yi; Jiang, Mengmeng; Le, Zhibiao; Tan, Shengxing

doi:10.1016/j.bbrc.2014.11.083

Cited by 38 publications

(35 citation statements)

References 36 publications

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“…It has been reported that miR-495 suppresses the post-transcription of forkhead box C1 (FOXC1) and thus inhibits endometrial cancer progression [23]. Also, miR-495 inhibits the migration and invasion of gastric cancer cells via interacting directly with phosphatase of regenerating liver-3 (PRL-3) [24,25]. Similar results also have been found in non-small cell lung cancer [26], leukemia [27], and prostate cancer [28].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-495 Inhibits New Bone Regeneration via Targeting High Mobility Group AT-Hook 2 (HMGA2)

Tian

Zhou

et al. 2017

Med Sci Monit

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BackgroundMicroRNAs play critical roles in post-translational gene expression. In this study, we explored the role of miR-495 in new bone regeneration.Material/MethodsMurine calvarial osteoblasts were isolated and cultured. Microarray was performed to identify differential miRNAs in medicarpin-induced osteoblasts differentiation. Luciferase reporter assay was performed to identify the target gene of miRNA. Murine osteoblast cells were transfected with miC, miR-495, or anti-miR-495. CCK-8 and flow cytometry were performed to detect osteoblasts proliferation and apoptosis. Western blot was used to analyze apoptosis-related proteins. qRT-PCR analysis was performed to detect gene expression. ALP activity and mineralized nodule formation test were used to evaluate bone formation. Dill-hole injury model was constructed and micro CT was utilized to measuring bone healing.ResultsMicroarray analysis identified miR-495 as our miRNA of interest and luciferase reporter assay identified HMGA2 as its target gene. Over-expression of miR-495 significantly inhibited ALP activity and mineralized nodule formation as well as the expression of RUNX-2, BMP-2, and Osterix. Also, miR-495 over-expression inhibited osteoblasts proliferation and promoted apoptosis obviously. In this in vivo study, the downregulation of miR-495 promoted murine femur healing.ConclusionsMiR-495 inhibits new bone regeneration via targeting high mobility group AT-Hook 2 (HMGA2). We propose that targeting miR-495 may be a promising therapeutic approach for bone regeneration.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-495 Inhibits New Bone Regeneration via Targeting High Mobility Group AT-Hook 2 (HMGA2)

Tian

Zhou

et al. 2017

Med Sci Monit

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“…Dysregulation of miRNAs has been reported in various human malignancies,13–15 and multiple deregulated miRNAs have been implicated in biological and pathological process of GC 16–20. Based on the deregulated miRNAs’ expression profiling and their association with the intrinsic characteristics of GC, specific miRNAs could be utilized as new noninvasive prognostic and diagnostic biomarkers for cancers 21,22.…”

Section: Discussionmentioning

confidence: 99%

Decreased miR-503 expression in gastric cancer is inversely correlated with serum carcinoembryonic antigen and acts as a potential prognostic and diagnostic biomarker

Wu¹,

Cao²,

Huang³

et al. 2016

OTT

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BackgroundAltered expression of miR-503 has been linked to human carcinogenesis. In this present study, we aimed to detect the potential for miR-503 as a novel biomarker for gastric cancer (GC) patients.Materials and methodsThe relative mRNA level of miR-503 in serum and tissue of 68 GC patients and serum of 32 healthy volunteers was determined by real-time reverse transcription quantitative polymerase chain reaction.ResultsThe miR-503 level was significantly lower in the tissue and serum of GC than their counterparts (all P<0.01). Downregulation of miR-503 was found to be corrected with more aggressive tumor. Patients in the high-miR-503 group showed significantly better overall survival compared to the low-miR-503 group (P=0.021). The serum miR-503 level in GC was inversely correlated with carcinoembryonic antigen (CEA) (r=−0.624, P<0.001). Furthermore, the area under the receiver operating characteristic curve for miR-503 discriminating GC patients from healthy individuals was 0.889 (P=0.006), with a sensitivity of 96.8% and a specificity of 79.4%, higher than that of CEA (area under the receiver operating characteristic curve =0.681, P=0.048).ConclusionThe present study suggests that the expression level of miR-503 may serve as prognostic and diagnostic biomarker for GC.

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“…Li et al [30] discovered that miRNA-495 down-regulates PRL-3 mRNA and protein levels in gastric cancer cells.…”

Section: Prl-3 Transcriptional Regulatorsmentioning

confidence: 99%

Regulatory mechanisms of phosphatase of regenerating liver (PRL)-3

Rubio

Köhn

2016

Biochemical Society Transactions

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The phosphatase of regenerating liver (PRL)-3 is overexpressed in many human cancer types and tumor metastases when compared with healthy tissues. Different pathways and mechanisms have been suggested to modulate PRL-3 expression levels and activity, giving some valuable insights but still leaving an incomplete picture. Investigating these mechanisms could provide new targets for therapeutic drug development. Here, we present an updated overview and summarize recent findings concerning the different PRL-3 expression regulatory mechanisms and posttranslational modifications suggested to modulate the activity, localization, or stability of this phosphatase.

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Methylation-associated silencing of miR-495 inhibit the migration and invasion of human gastric cancer cells by directly targeting PRL-3

Cited by 38 publications

References 36 publications

MicroRNA-495 Inhibits New Bone Regeneration via Targeting High Mobility Group AT-Hook 2 (HMGA2)

MicroRNA-495 Inhibits New Bone Regeneration via Targeting High Mobility Group AT-Hook 2 (HMGA2)

Decreased miR-503 expression in gastric cancer is inversely correlated with serum carcinoembryonic antigen and acts as a potential prognostic and diagnostic biomarker

Regulatory mechanisms of phosphatase of regenerating liver (PRL)-3

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