Méthylation du spermatozoïde en Assistance médicale à la procréation (AMP)

Benchaïb, Mehdi; Ajina, Mounir; Braun, Valerie; Niveleau, Alain; Guérin, J. F.

doi:10.1016/j.gyobfe.2006.07.036

Cited by 1 publication

(1 citation statement)

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“…DNA methylation is another epigenetic process during spermatogenesis that also affects the clinical results of ART and is linked with embryonic development. Previous reports have shown that the pregnancy rate is higher when sperm DNA methylation is above a certain threshold[ 14 , 15 ]. Infertile men, especial oligozoospermic patients, often undergo aberrant DNA methylation of imprinted loci in sperm,which in turn can be passed on to their offspring[ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction

Yu¹,

Zhou²,

Liu³

et al. 2015

PLoS ONE

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Epidemiological evidence indicates that assisted reproductive technologies (ART) may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS) or Silver-Russell syndrome (SRS). Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality; however, epigenetic alterations associated with this approach are largely unknown. In the present study, we investigated DNA methylation and histone retention profiles in raw sperm and selected sperm derived from the same individual and separated by using density-gradients. Results from a study group consisting of 93 males demonstrated that both global DNA methylation and histone retention levels decreased in density selected sperm. Compared to unselected raw sperm, histone transition rates decreased by an average of 27.2% in selected sperm, and the global methylation rate was 3.8% in unselected sperm and 3.3% in the selected sperm. DNA methylation and histone retention location profiling analyses suggested that these alterations displayed specific location patterns in the human genome. Changes in the pattern of hypomethylation largely occurred in transcriptional factor gene families such as HOX, FOX, and GATA. Histone retention increased in 67 genes, whereas it was significantly clustered in neural development-related gene families, particularly the olfactory sensor gene family. Although a causative relationship could not be established, the results of the present study suggest the possibility that sperm with good density also possess unique epigenetic profiles, particularly for genes involved in neural and olfactory development. As increasing evidence demonstrates that epigenetics plays a key role in embryonic development and offspring growth characteristics, the specific epigenetic alterations we observed in selected sperm may influence the transcriptional process and neural development in embryos.

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Section: Introductionmentioning

confidence: 99%