Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage

Lees-Murdock, Diane J.; Felici, Massimo De; Walsh, Colum P.

doi:10.1016/s0888-7543(03)00105-8

Cited by 145 publications

(123 citation statements)

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“…In this line, one of the main contributions of our study is that we have identified this small but dynamic proportion. It is believed that demethylation and remethylation of repeats is mostly completed before birth 9,[11][12][13] . In the present study, we show that the 5hmC status in repeats keep changing during spermatogenesis even after birth.…”

Section: Article Nature Communications | Doi: 101038/ncomms2995mentioning

confidence: 99%

“…In mice, the majority of methylation in male germ cells is completed before type A SG (SG-A) are formed at 6 days post-partum (dpp), while those at a small number of sites continues to occur until the formation of pachytene SC (pacSC) 10 . Imprinted genes, repetitive sequences and non-promoter intergenic regions complete DNA methylation before birth 9,[11][12][13] . However, little is known about whether and how global DNA methylation changes in germ cells after birth.…”

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confidence: 99%

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Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis

et al. 2013

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Little is known about how patterns of DNA methylation change during mammalian spermatogenesis. 5hmC has been recognized as a stable intermediate of DNA demethylation with potential regulatory functions in the mammalian genome. However, its global pattern in germ cells has yet to be addressed. Here, we first conducted absolute quantification of 5hmC in eight consecutive types of mouse spermatogenic cells using liquid chromatographytandem mass spectrometry, and then mapped its distributions in various genomic regions using our chemical labeling and enrichment method coupled with deep sequencing. We found that 5hmC mapped differentially to and changed dynamically in genomic regions related to expression regulation of protein-coding genes, piRNA precursor genes and repetitive elements. Moreover, 5hmC content correlated with the levels of various transcripts quantified by RNA-seq. These results suggest that the highly ordered alterations of 5hmC in the mouse genome are potentially crucial for the differentiation of spermatogenic cells.

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Section: Article Nature Communications | Doi: 101038/ncomms2995mentioning

confidence: 99%

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confidence: 99%

Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis

et al. 2013

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“…Bisulfite sequencing showed that imprinted genes, including Peg3, Kcnq1ot1 (also known as Lit 1), Snrpn, H19, Rasgrf1 and Gtl2, as well as non-imprinted genes, such as α-actin, become demethylated between 10.5 and 13.5 days of gestation (Hajkova et al 2002;Li et al 2004). Certain sequences, such as the repetitive elements intracisternal A particle (IAP), long interspersed nuclear element 1 (LINE1) and minor satellites, may be treated differently because they appear to be only partially demethylated in PGCs (Hajkova et al 2002;Szabo et al 2002;Lane et al 2003;Lees-Murdock et al 2003).…”

Section: Erasure Of Methylation Patterns In the Germ Linementioning

confidence: 99%

Gamete imprinting: setting epigenetic patterns for the next generation

Trasler¹

2006

Reprod. Fertil. Dev.

120

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Abstract. The acquisition of genomic DNA methylation patterns, including those important for development, begins in the germ line. In particular, imprinted genes are differentially marked in the developing male and female germ cells to ensure parent-of-origin-specific expression in the offspring. Abnormalities in imprints are associated with perturbations in growth, placental function, neurobehavioural processes and carcinogenesis. Based, for the most part, on data from the well-characterised mouse model, the present review will describe recent studies on the timing and mechanisms underlying the acquisition and maintenance of DNA methylation patterns in gametes and early embryos, as well as the consequences of altering these patterns.

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“…Establishment of new imprints by de-novo methylation of the DMR occurs in the prospermatogonia in the male germ line and during the oocyte growth phase in the ovary (Davis et al, 2000;Ueda et al, 2000;Lucifero et al, 2004;Li et al, 2004). Methylation of intracisternal A particles (IAP), LINE1 elements (L1) and minor satellite repeats also decreases in post-migratory germ cells but is not wholly removed (Hajkova et al, 2002;Lees-Murdock et al, 2003), which is important for maintaining transcriptional repression of transposons (Bourc'his and Bestor, 2004;Webster et al, 2005) and probably for the stability of the minor satellite DNA; remethylation of any demethylated elements also occurs in the prospermatogonia in males and the growing oocyte in females (Lees-Murdock et al, 2003;Lucifero et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Sex-specific promoters regulate Dnmt3L expression in mouse germ cells

et al. 2006

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BACKGROUND: Dnmt3L, a member of the DNA methyltransferase 3 family, lacks enzymatic activity but is required for de-novo methylation of imprinted genes in oocytes and for transposon repression in male germ cells. METHODS: We used northern blots, RT-PCR, 5¢ rapid amplification of complementary DNA (cDNA) ends (RACE), RNase H mapping, real-time/quantitative RT-PCR and in situ hybridization to identify and characterize Dnmt3L transcripts produced during germ cell development. RESULTS: Mouse Dnmt3L uses three sex-specific promoters, not the single promoter previously thought. A promoter active in prospermatogonia drives transcription of an mRNA encoding the full-length protein in perinatal testis, where de-novo methylation occurs. Late pachytene spermatocytes activate a second promoter in intron 9 of the Dnmt3L gene. After this stage, the predominant transcripts are three truncated mRNAs, which appear to be non-coding. We could also detect similar adult testis transcripts in humans. In the mouse ovary, an oocyte-specific promoter located in an intron of the neighbouring autoimmune regulator (Aire) gene produces a transcript with the full open reading frame (ORF). This is the only Dnmt3L transcript found in growing oocytes and is absent in the oocytes of Dnmt3L-/-females. CONCLUSIONS: Sex-specific promoters control Dnmt3L expression in the mouse germ line, mirroring the situation at the Dnmt1 and Dnmt3A loci.

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Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage

Cited by 145 publications

References 40 publications

Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis

Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis

Gamete imprinting: setting epigenetic patterns for the next generation

Sex-specific promoters regulate Dnmt3L expression in mouse germ cells

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