Methylation of elongation factor 1A by yeast Efm4 or human eEF1A-KMT2 involves a beta-hairpin recognition motif and crosstalks with phosphorylation

Hamey, Joshua J.; Nguyen, Amy; Haddad, Mahdi; Vázquez-Campos, Xabier; Pfeiffer, Paige G.; Wilkins, Marc R.

doi:10.1016/j.jbc.2024.105639

Cited by 3 publications

References 91 publications

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Saccharomyces Genome Database: advances in genome annotation, expanded biochemical pathways, and other key enhancements

Engel,

Aleksander,

Nash

et al. 2024

Genetics

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Budding yeast (Saccharomyces cerevisiae) is the most extensively characterized eukaryotic model organism and has long been used to gain insight into the fundamentals of genetics, cellular biology, and the functions of specific genes and proteins. The Saccharomyces Genome Database (SGD) is a scientific resource that provides information about the genome and biology of S. cerevisiae. For more than 30 years, SGD has maintained the genetic nomenclature, chromosome maps, and functional annotation for budding yeast along with search and analysis tools to explore these data. Here we describe recent updates at SGD, including the two most recent reference genome annotation updates, expanded biochemical pathways representation, changes to SGD search and data files, and other enhancements to the SGD website and user interface. These activities are part of our continuing effort to promote insights gained from yeast to enable the discovery of functional relationships between sequence and gene products in fungi and higher eukaryotes.

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Saccharomyces Genome Database: advances in genome annotation, expanded biochemical pathways, and other key enhancements

Engel,

Aleksander,

Nash

et al. 2024

Genetics

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show abstract

SaccharomycesGenome Database: Advances in Genome Annotation, Expanded Biochemical Pathways, and Other Key Enhancements

Engel,

Aleksander,

Nash

et al. 2024

Preprint

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Budding yeast (Saccharomyces cerevisiae) is the most extensively characterized eukaryotic model organism and has long been used to gain insight into the fundamentals of genetics, cellular biology, and the functions of specific genes and proteins. TheSaccharomycesGenome Database (SGD) is a scientific resource that provides information about the genome and biology ofS. cerevisiae. For more than 30 years, SGD has maintained the genetic nomenclature, chromosome maps, and functional annotation for budding yeast along with search and analysis tools to explore these data. Here we describe recent updates at SGD, including the two most recent reference genome annotation updates, expanded biochemical pathways representation, changes to SGD search and data files, and other enhancements to the SGD website and user interface. These activities are part of our continuing effort to promote insights gained from yeast to enable the discovery of functional relationships between sequence and gene products in fungi and higher eukaryotes.

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SMYD5 is a ribosomal methyltransferase which trimethylates RPL40 lysine 22 through recognition of a KXY motif

Hamey,

Shah,

Wade

et al. 2024

Preprint

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The eukaryotic ribosome is highly modified by protein methylation, yet many of the responsible methyltransferases remain unknown. Here we have identified SMYD5 as a ribosomal protein methyltransferase that catalyses trimethylation of RPL40/eL40 at lysine 22. Through a systematic mass spectrometry-based approach, we show that the human ribosome has 12 primary sites of protein methylation, including at RPL40 K22. Through in vitro methylation of synthetic RPL40 using fractionated lysate, we then identify SMYD5 as a candidate RPL40 K22 methyltransferase. We show that recombinant SMYD5 has robust activity towards RPL40 K22 in vitro, and that active site mutations ablate this activity. Knockouts of SMYD5 in K562 cells show a complete loss of RPL40 K22 methylation and decrease polysome levels. By systematic analysis of its recognition motif, we show that SMYD5 requires a tyrosine in the +2 position, and thereby is incapable of methylating its previously reported histone substrates.

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Methylation of elongation factor 1A by yeast Efm4 or human eEF1A-KMT2 involves a beta-hairpin recognition motif and crosstalks with phosphorylation

Cited by 3 publications

References 91 publications

Saccharomyces Genome Database: advances in genome annotation, expanded biochemical pathways, and other key enhancements

Saccharomyces Genome Database: advances in genome annotation, expanded biochemical pathways, and other key enhancements

SaccharomycesGenome Database: Advances in Genome Annotation, Expanded Biochemical Pathways, and Other Key Enhancements

SMYD5 is a ribosomal methyltransferase which trimethylates RPL40 lysine 22 through recognition of a KXY motif

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