Methylation pattern variation between goats and rats during the onset of puberty

Yang, Chen; Ye, J; Liu, Y; Ding, Jianping; Liu, H; Gao, Xiaoxiao; Li, X; Zhang, Y; Zhou, Jie; Zhang, X; Huang, Weiping; Fang, Fugui; Ling, Yinghui

doi:10.1111/rda.13172

Cited by 12 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This decrease of the global DNA methylation, from Pre- to In-pubertal stage, may be related to the DNA methylation changes of genes which represses the initiating of puberty in Pre-puberty, and these genes may change from transcriptional repressions to activations. Moreover, many DMGs such as NLRC5, SMOC1, GRID1, ABAT, MAP3K4, and PTPRN reported in rat (Yang et al, 2018) and goat (Yang et al, 2018) across the pubertal transition were also identified in this study (Supplementary File S4). Furthermore, changes of the DNA methylation in the hypothalamus were all enriched in Neurotrophin signaling pathway and Estrogen signaling pathway for rat (Yang et al, 2018), goat (Yang et al, 2018)and pigs (Figure 6).…”

Section: Discussionsupporting

confidence: 54%

“…Moreover, many DMGs such as NLRC5, SMOC1, GRID1, ABAT, MAP3K4, and PTPRN reported in rat (Yang et al, 2018) and goat (Yang et al, 2018) across the pubertal transition were also identified in this study (Supplementary File S4). Furthermore, changes of the DNA methylation in the hypothalamus were all enriched in Neurotrophin signaling pathway and Estrogen signaling pathway for rat (Yang et al, 2018), goat (Yang et al, 2018)and pigs (Figure 6). These observations not only supported the accuracies of findings in the present study but also suggested the patterns of DNA methylation variations were like to be similar among goat, rat and pigs.…”

Section: Discussionsupporting

confidence: 54%

“…Recently, the genome-wide DNA methylation changes of hypothalamus have been investigated during the timing of puberty in rat (Luo et al, 2017) and goat (Yang et al, 2016), even the methylation variation between rat and goat (Yang et al, 2018). Results of rat (Luo et al, 2017) and goat (Yang et al, 2016) show that the global DNA methylation level is decreasing across Pre- to In-pubertal stages, which is in line with the present study.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Genome-Wide DNA Methylation Analysis of Hypothalamus During the Onset of Puberty in Gilts

et al. 2019

View full text Add to dashboard Cite

Although selection of the early age at puberty in gilts will make for a favorable effect on the reproductivity of sow, a large proportion of phenotypic variation in age at puberty of gilts cannot be explained by genetics. Previous studies have implicated hypothalamic DNA methylation in the onset of puberty in mammals. However, the underlying molecular mechanism regarding the regulation of the onset of puberty has remained largely unexplored in gilts. Herein, the genome-scale DNA methylation of hypothalamus was acquired, using the reduced representation bisulfite sequencing, to compare and describe the changes of DNA methylation across Pre-, In- and Post-pubertal gilts. In this study, the average methylation levels of CpGs and CpHs (where H = C, T, or A) in CpG islands- and gene-related regions were gradually decreased in hypothalamic methylomes during the pubertal transition. Comparisons of Pre- vs. In-, In- vs. Post-, and Pre- vs. Post-pubertal stage revealed that there were 85726, 92914, and 100421 differentially methylated CpGs and 5940, 14804, and 16893 differentially methylated CpHs (where H = C, T, or A) in the hypothalamic methylomes. The methylation changes of CpHs were more dynamic than that of CpGs, and methylation changes of CpGs and CpHs were likely to be, respectively, involved in the developmental processes of reproduction and the molecular processes of cellular communications in the hypothalamus. Moreover, methylation changes of CpHs were observed to overrepresent in the quantitative trait loci of age at puberty, and the biological function of these CpH methylation changes was enriched in the pancreas development in gilts. Furthermore, the mRNA levels of several differentially CpG or CpH methylated genes related to the transcription of RNA II polymerase, GnRH signaling pathway, Estrogen signaling pathway, PI3K-AKt signaling pathway, and Insulin signaling pathway, including MAX, MMP2, FGF11, IGF1R, FGF21, and GSK3B, were significantly changed across these pubertal stages in the hypothalamus. These results will help our understanding of how DNA methylation contributes to phenotypic variation of age at puberty.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide DNA Methylation Analysis of Hypothalamus During the Onset of Puberty in Gilts

et al. 2019

View full text Add to dashboard Cite

show abstract

“…GABA is known to regulate reproduction, but it is not known whether GABA-T affects reproductive function by affecting GABA. GABAergic signaling pathways exhibited an association with the initiation of puberty, and GABA-T was significantly increased in pubertal goats compared to prepubertal goats [25,26]. Therefore, we speculated that GABA-T affects reproductive function.…”

Section: Introductionmentioning

confidence: 98%

Effect of GABA-T on Reproductive Function in Female Rats

Kang

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

This study explored the role of γ-aminobutyric acid transaminase (GABA-T) in the puberty and reproductive performance of female rats. Immunofluorescence technique, quantitative real-time PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the distribution of GABA-T and the expression of genes and hormones in female rats, respectively. The results showed that GABA-T was mainly distributed in the arcuate nucleus (ARC), paraventricular nucleus (PVN) and periventricular nucleus (PeN) of the hypothalamus, and in the adenohypophysis, ovarian granulosa cells and oocytes. Abat mRNA level at 28 d was lowest in the hypothalamus and the pituitary; at puberty, it was lowest in the ovary. Abat mRNA level was highest in adults in the hypothalamus; at infancy and puberty, it was highest in the pituitary; and at 21 d it was highest in the ovary. After vigabatrin (GABA-T irreversible inhibitor) was added to hypothalamus cells, the levels of Abat mRNA and Rfrp-3 mRNA were significantly reduced, but Gnrh mRNA increased at the dose of 25 and 50 μg/mL; Kiss1 mRNA was significantly increased but Gabbr1 mRNA was reduced at the 50 μg/mL dose. In prepubertal rats injected with vigabatrin, puberty onset was delayed. Abat mRNA, Kiss1 mRNA and Gnrh mRNA levels were significantly reduced, but Rfrp-3 mRNA level increased in the hypothalamus. Vigabatrin reduced the concentrations of GABA-T, luteinizing hormone (LH) and progesterone (P4), and the ovarian index. Lactation performance was reduced in adult rats with vigabatrin treatment. Four hours after vigabatrin injection, the concentrations of GABA-T and LH were significantly reduced in adult and 25 d rats, but follicle-stimulating hormone (FSH) increased in 25 d rats. In conclusion, GABA-T affects the reproductive function of female rats by regulating the levels of Gnrh, Kiss1 and Rfrp-3 in the hypothalamus as well as the concentrations of LH and P4.

show abstract

“…Previous studies have reported that changes of DNA methylation cause KISS1 gene, which has emerged as an essential gatekeeper for the onset of puberty via directing the stimulation of GnRH secretion at the hypothalamic level [20–22], switching from repressive to permissive in mice [19, 23, 24]. Moreover, the changes and dynamics of genome-wide DNA methylation during the onset of puberty have been described for the hypothalamus of female goats [25, 26] and rats [27], and these studies have provided useful insights into the epigenetic mechanism for the timing of puberty at hypothalamus level for mammals. However, few investigations have focused on the dynamics and changes of DNA methylation in pituitaries during the pubertal transition in mammals.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide DNA methylation analysis of pituitaries during the initiation of puberty in gilts

Yuan

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

It has been widely recognized that the early or delayed puberty appears to display harmful effects on adult health outcomes. During the timing of puberty, pituitaries responds to the hypothalamus and then introduce the following response of ovaries in hypothalamic-pituitary-gonadal axis. DNA methylation has been recently suggested to regulate the onset of puberty in female mammals. However, to date, the changes of DNA methylation in pituitaries have not been investigated during pubertal transition. In this study, using gilts as the pubertal model, the genome-scale DNA methylation of pituitaries was profiled and compared across Pre-, In- and Post-puberty by using the reduced representation bisulfite sequencing. We found that average methylation levels of each genomic feature in Post- were lower than Pre- and In-pubertal stage in CpG context, but they were higher in In- than that in Pre- and Post-pubertal stage in CpH (where H = A, T, or C) context. The methylation patterns of CpHs were more dynamic than that of CpGs at the location of high CpG content, low CpG content promoter genes, and differently genomic CGIs. Furthermore, the differently genomic CGIs were likely to show in a similar manner in CpG context but display in a stage-specific manner in the CpH context across the Pre-, In- and Post-pubertal stage. Among these pubertal stages, 5 kb upstream regions of the transcription start sites were protected from both CpG and CpH methylation changes. 12.65% of detected CpGs were identified as the differentially methylated CpGs, regarding 4301 genes which were involved in the fundamental functions of pituitaries. 0.35% of detected CpHs were identified as differentially methylated CpHs, regarding 3691 genes which were involved in the biological functions of releasing gonadotropin hormones. These observations and analyses would provide valuable insights into epigenetic mechanism of the initiation of puberty in pituitary level.

show abstract

Methylation pattern variation between goats and rats during the onset of puberty

Cited by 12 publications

References 28 publications

Genome-Wide DNA Methylation Analysis of Hypothalamus During the Onset of Puberty in Gilts

Genome-Wide DNA Methylation Analysis of Hypothalamus During the Onset of Puberty in Gilts

Effect of GABA-T on Reproductive Function in Female Rats

Genome-wide DNA methylation analysis of pituitaries during the initiation of puberty in gilts

Contact Info

Product

Resources

About