Methylation profile of SNRPN gene and its correlation with weight and chronological age

Abstract

ABSTRACT. Genomic imprinting is an important epigenetic phenomenon, wherein genes or gene clusters are marked by DNA methylation during gametogenesis. This plays a major role in several functions of normal cells, including cell differentiation, X chromosome inactivation, and the maintenance of chromatin structure, in mammalian development. The aim of this study was to investigate the possible differences in SNRPN gene methylation profiles in non-obese and obese individuals, and in children and adults. Our results did not reveal any statistical correlations between the DNA methylation profiles of the SNRPN gene in children or adult obese and non-obese groups. However, a comparison of the methylation levels with the chronological age revealed statistically significant differences between the means of methylation in adults and children (46.20 ± 5.88 and 39.40 ± 2.87, respectively; P < 0.001). Pearson's correlation analysis indicated a positive association between the level of DNA methylation and the chronological age (R 2 = 0.326; P < 0.001). Therefore, we concluded that the methylation profile of the SNRPN promoter (in blood) is not a useful biomarker for determining the predisposition of an individual to obesity. Additionally, we have confirmed that SNRPN methylation increases with 13791-13798 (2015) age, which raises further questions regarding the role of SNRPN expression during the aging process.

Polymorphisms in the SNRPN gene are associated with obesity susceptibility in a Spanish population

“…This is an intersting consideration for further analysis. In this regard, a previous study by Carobin et al 23 investigating the possible differences in SNRPN gene methylation profiles in non-obese and obese individuals did not find any significant results. However, Soubry et al 24 suggest that a contribution of paternal obesity can change epigenetic programming in a small fraction of sperm cells.…”

“…This is an intersting consideration for further analysis. In this regard, a previous study by Carobin et al . investigating the possible differences in SNRPN gene methylation profiles in non‐obese and obese individuals did not find any significant results.…”

Small Nuclear Ribonucleoprotein Polypeptide N Accelerates Malignant Progression and Poor Prognosis in Colorectal Cancer Transcriptionally Regulated by E2F8

“…SNRPN hyper-methylation was observed in some diseases, 34.9% in myelodysplastic syndrome and 50% in acute myeloid leukemia (22). The methylation levels of SNRPN revealed statistical differences between adults and children, which indicates the role of SNRPN during the aging process (23). Differential methylation patterns of SNRPN have also been reported in multiple types of cancer.…”

Caracterização de uma amostra de indivíduos com deficiência intelectual de origem indeterminada

“…O gene SNRPN (MIM 182279) codifica dois tipos diferentes de proteína, Snurf e SmN, essa última é relacionada ao processamento pré-mRNA e pode contribuir para o splicing alternativo tecido-específico 333 . Em condições normais, esse gene encontra-se metilado no alelo materno, com metilação diferenciada de acordo com a idade, sugerindo uma função adicional no processo de envelhecimento [334][335] .…”