2021
DOI: 10.1007/s11248-020-00224-9
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Methylation silencing and reactivation of exogenous genes in lentivirus-mediated transgenic mice

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Cited by 5 publications
(5 citation statements)
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“…This examination was performed using the qRT-PCR method implemented on selected YhFF#8-Cas9-GFP-PuroR cells, and the results showed that Cas9 was expressed at 50% of the level of GFP and the GAPDH housekeeping gene. The performance values obtained in this study are consistent with those of other studies in which the EF-1-α promoter outperformed the CMV promoter by 40-60% (27,31).…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…This examination was performed using the qRT-PCR method implemented on selected YhFF#8-Cas9-GFP-PuroR cells, and the results showed that Cas9 was expressed at 50% of the level of GFP and the GAPDH housekeeping gene. The performance values obtained in this study are consistent with those of other studies in which the EF-1-α promoter outperformed the CMV promoter by 40-60% (27,31).…”
Section: Discussionsupporting
confidence: 91%
“…The genetically modified YhFF#8 expressing Cas9 gene resistance gene (shown in Figure 1A). CMV is a promoter derived from cytomegalovirus, while EF-1-α is a eukaryotic and human promoter (26,27). Although measures have been taken to ensure the proper function of these promoters, some reports have indicated the silencing of genes transferred by pseudo-virus vectors after a certain number of cell divisions (starting from the 4 th wk of transduction) (28)(29)(30).…”
Section: Discussionmentioning
confidence: 99%
“…While Tfap2c was introduced into Col1A1 locus, the remaining factors were delivered into the genome by lentiviral transduction thereby being random in site and number of integrations. Further variations might result from methylation-induced transgene silencing which was shown to occur with transgenes delivered by lentiviral vectors albeit less frequently than following simple retrovirus transduction (Pfeifer et al, 2002;Wen et al, 2021).…”
Section: Tx Medium Promotes Esc To Tsc Conversionmentioning
confidence: 99%
“…Cell culture experiments aiming at high gene expression employ transposable elements to achieve stable single-copy integrations or induce high-copy amplification in combination with measures to avoid RIGS. These measures may include chromatin relaxation by inhibitors (trichostatin A, butyrate [ 119 ]; 5-azacytidine (5-AzaC) [ 128 ] or attaching functional elements to the transgene (BAC genomic regions, matrix attachment regions (MARs)) [ 12 , 109 , 129 , 130 ].…”
Section: Repeat-induced Gene Silencingmentioning
confidence: 99%