Methylation status in the promoter region of the human PGP9.5 gene in cancer and normal tissues

Rosas, Siane Lopes Bittencourt; Caballero, Otávia L.; Dong, Seung Myung; Carvalho, Maria da Glória da Costa; Sidransky, David; Jen, Jin

doi:10.1016/s0304-3835(01)00449-9

Cited by 35 publications

(20 citation statements)

References 22 publications

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“…6B, arrow). Disulfide crosslinking sites in NT-UCH-L1 multimer were also identified by peptide sequencing with nonoUPLC ESI-q-TOF MS/MS combined with the searching algorithm of disulfide site, DBond [54]. We detected many disulfide containing peptides (Table 1).…”

Section: Resultsmentioning

confidence: 98%

“…We therefore compared the subcellular localizations of UCH-L1 and NT-UCH-L1. We used HeLa cells because the expression of endogenous UCH-L1 in these cells was blocked by methylation of the UCH-L1 gene [54]. In confocal microscopy, the majority of UCH-L1-myc localizedin the cytosol, while NT-UCH-L1-myc localized in both cytosol and mitochondria (Fig.…”

Section: Resultsmentioning

confidence: 99%

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N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage

Kim

Jeong

et al. 2014

PLoS ONE

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Ubiquitin C-terminal hydrolase-L1 (UCH-L1) has been proposed as one of the Parkinson's disease (PD) related genes, but the possible molecular connection between UCH-L1 and PD is not well understood. In this study, we discovered an N-terminal 11 amino acid truncated variant UCH-L1 that we called NT-UCH-L1, in mouse brain tissue as well as in NCI-H157 lung cancer and SH-SY5Y neuroblastoma cell lines. In vivo experiments and hydrogen-deuterium exchange (HDX) with tandem mass spectrometry (MS) studies showed that NT-UCH-L1 is readily aggregated and degraded, and has more flexible structure than UCH-L1. Post-translational modifications including monoubiquitination and disulfide crosslinking regulate the stability and cellular localization of NT-UCH-L1, as confirmed by mutational and proteomic studies. Stable expression of NT-UCH-L1 decreases cellular ROS levels and protects cells from H2O2, rotenone and CCCP-induced cell death. NT-UCH-L1-expressing transgenic mice are less susceptible to degeneration of nigrostriatal dopaminergic neurons seen in the MPTP mouse model of PD, in comparison to control animals. These results suggest that NT-UCH-L1 may have the potential to prevent neural damage in diseases like PD.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage

Kim

Jeong

et al. 2014

PLoS ONE

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“…(4)). Despite being linked to Parkinson's disease, UCH-L1 is also expressed in many primary lung tumours and lung cancer cell lines, but not normal lung tissue [46][47], where it is proposed to be linked to tumour progression upon upregulation [44]. Expression of UCH-L1 also correlates with tumour progression in colorectal cancer [48].…”

Section: N-alkyl Substituted Isatin Derivativesmentioning

confidence: 99%

Recent Highlights in the Development of Isatin-Based Anticancer Agents

Vine¹,

Matesic²,

Locke³

et al. 2013

Advances in Anticancer Agents in Medicinal Chemistry

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Isatin (1H-indole-2,3-dione) and its derivatives are responsible for a broad spectrum of biological activities. Among these the cytotoxic and antineoplastic properties have been the most widely reported. The synthetic versatility of the isatin, due to its privileged scaffold, has led to the generation of a large number of structurally diverse derivatives which include analogues derived from either mono-, di-, and trisubstitution of the aryl ring A, and/or those obtained by derivatisation of the isatin nitrogen and C2/C3 carbonyl moieties. These compounds inhibit cancer cell proliferation and tumour growth via interaction with a variety of intracellular targets such as DNA, telomerase, tubulin, P-glycoprotein, protein kinases and phosphatases. Herein we review recent highlights in the development of isatin-based compounds as anticancer agents with a particular focus on the cytotoxicity and structure activity relationships. Abstract:Isatin (1H-indole-2,3-dione) and its derivatives are responsible for a broad spectrum of biological activities. Among these the cytotoxic and antineoplastic properties have been the most widely reported. The synthetic versatility of the isatin, due to its privileged scaffold, has led to the generation of a large number of structurally diverse derivatives which include analogues derived from either mono-, di-, and tri-substitution of the aryl ring A, and/or those obtained by derivatisation of the isatin nitrogen and C2/C3 carbonyl moieties. These compounds inhibit cancer cell proliferation and tumour growth via interaction with a variety of intracellular targets such as DNA, telomerase, tubulin, Pglycoprotein, protein kinases and phosphatases. Herein we review recent highlights in the development of isatin-based compounds as anticancer agents with a particular focus on the cytotoxicity and structure activity relationships.

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“…Using the SAGE, it was initially found that the PGP9.5 transcript and protein were highly expressed in more than 50% of primary lung cancers and nearly all lung cancer cell lines but was not detectable in the normal lung [36]. This increased expression could be the result of transcriptional regulation accompanied by methylation changes at the CpG island of the promoter region.…”

Section: Sage In Human Studiesmentioning

confidence: 99%

“…Exposure of HeLa cells to the demethylating agent, 5-aza-2′-deoxycytidine, led to re-expression of PGP9.5. This data suggested that while other mechanisms may be involved in the frequent overexpression of PGP9.5 gene in lung tumors and lung cancer cell lines, promoter methylation may play a role in the transcriptional suppression of PGP9.5 gene expression in the cervical tissue-derived HeLa cell line [36]. …”

Section: Sage In Human Studiesmentioning

confidence: 99%

Serial Analysis of Gene Expression: Applications in Human Studies

Tuteja

2004

BioMed Research International

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Serial analysis of gene expression (SAGE) is a powerful tool, which provides quantitative and comprehensive expression profile of genes in a given cell population. It works by isolating short fragments of genetic information from the expressed genes that are present in the cell being studied. These short sequences, called SAGE tags, are linked together for efficient sequencing. The frequency of each SAGE tag in the cloned multimers directly reflects the transcript abundance. Therefore, SAGE results in an accurate picture of gene expression at both the qualitative and the quantitative levels. It does not require a hybridization probe for each transcript and allows new genes to be discovered. This technique has been applied widely in human studies and various SAGE tags/SAGE libraries have been generated from different cells/tissues such as dendritic cells, lung fibroblast cells, oocytes, thyroid tissue, B-cell lymphoma, cultured keratinocytes, muscles, brain tissues, sciatic nerve, cultured Schwann cells, cord blood-derived mast cells, retina, macula, retinal pigment epithelial cells, skin cells, and so forth. In this review we present the updated information on the applications of SAGE technology mainly to human studies.

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Methylation status in the promoter region of the human PGP9.5 gene in cancer and normal tissues

Cited by 35 publications

References 22 publications

N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage

N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage

Recent Highlights in the Development of Isatin-Based Anticancer Agents

Serial Analysis of Gene Expression: Applications in Human Studies

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