Methylene blue intercalated aptamer to amplify signals toward sensitively electrochemical detection of dopamine released from living Parkinson's disease model cells

Tang, Chuyue; Zou, Zhuo; Liang, Taotao; Yuan, Chengsong; Gao, Jiechang; Tang, Kanglai; Li, Chang Ming

doi:10.1016/j.snr.2022.100080

Cited by 11 publications

(6 citation statements)

References 52 publications

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“…One label-free electrochemical sensing method for disease detection leverages the electrostatic affinity or intercalation ability of cationic redox probes, such as MB, ruthenium (III) [Ru(III)] hexamine, or hemin, to the negatively charged backbone of nucleic acid-based biorecognition probes, which are most often aptamers. Some redox probes, like MB and hemin, intercalate into double-stranded nucleic acids and can specifically bind to guanine (G) base pairs (53)(54)(55). Therefore, many biorecognition probes or amplification assay products incorporate G-triplex (56) or G-quadruplex (55, 57) for improved intercalation.…”

Section: Intercalation Ability and Electrostatic Affinity Of Redox Pr...mentioning

confidence: 99%

Label-Free Electrochemical Methods for Disease Detection

Rahn

Peramune

Zhang

et al. 2023

Annual Rev. Anal. Chem.

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Label-free electrochemical biosensing leverages the advantages of label-free techniques, low cost, and fewer user steps, with the sensitivity and portability of electrochemical analysis. In this review, we identify four label-free electrochemical biosensing mechanisms: ( a) blocking the electrode surface, ( b) allowing greater access to the electrode surface, ( c) changing the intercalation or electrostatic affinity of a redox probe to a biorecognition unit, and ( d) modulating ion or electron transport properties due to conformational and surface charge changes. Each mechanism is described, recent advancements are summarized, and relative advantages and disadvantages of the techniques are discussed. Furthermore, two avenues for gaining further diagnostic information from label-free electrochemical biosensors, through multiplex analysis and incorporating machine learning, are examined. Expected final online publication date for the Annual Review of Analytical Chemistry, Volume 16 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Intercalation Ability and Electrostatic Affinity Of Redox Pr...mentioning

confidence: 99%

Label-Free Electrochemical Methods for Disease Detection

Rahn

Peramune

Zhang

et al. 2023

Annual Rev. Anal. Chem.

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“…On account of their specific structure and functions, phenothiazine compounds have been utilized widely in many areas. Over one hundred species of related compounds have been established as clinical pharmaceuticals, revealing promising potential in the community of anticancer, antiviral (including Covid-19 , ), antipsychotic, and Alzheimer’s disease clinical treatment. Meanwhile, the phenothiazine structural moiety persistently appears in the research field of photoelectric materials, fluorescence probes, and photocatalysis (Figure ).…”

Section: Introductionmentioning

confidence: 99%

Oxidative Dehydroxycyclization of Catechols with o-Mercaptoanilines to Access 1-Hydroxyphenothiazines

Zhou,

Shang,

et al. 2024

J. Org. Chem.

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The protocol of aerobic oxidative dehydroxycyclization installed in the synthesis of rarely studied 1hydroxyphenothiazines from catechols and o-mercaptoanilines is presented. Utilizing a natural renewable low-toxicity gallic acid as an organocatalyst, this established transformation proceeded smoothly in an aqueous ethanol solution under mild conditions with good functional group compatibility and up to a 94% isolated yield. This protocol is also characterized by its operational simple workup involving only recrystallization, revealing its sustainability and synthetic practicability.

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“…At the physiological pH, MB undergoes a redox reaction 31 with a decreased redox signal when MB is bound to dsDNA compared to ssDNA. 32,33…”

Section: Introductionmentioning

confidence: 99%

“…At the physiological pH, MB undergoes a redox reaction 31 with a decreased redox signal when MB is bound to dsDNA compared to ssDNA. 32,33 In this study, an electrochemical technique using an aptamer to detect electron transfer flavoprotein subunit beta (ETFB) of Leptospira interrogans for diagnosis of leptospirosis was developed. ETFB has been reported as a candidate protein for leptospirosis diagnosis.…”

Section: Introductionmentioning

confidence: 99%